The structure of a polysaccharide from infectious strains of Burkholderia cepacia

Linker, Alfred; Evans, Leigh R.; Impallomeni, Giuseppe

doi:10.1016/s0008-6215(01)00205-1

Cited by 98 publications

(38 citation statements)

References 25 publications

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“…These results correspond to reports of a highly branched heptasaccharide repeating unit with a similar composition, with O-acetyl groups, and produced by clinical BCC isolates (5,20). Strain C1394 was shown, in addition to the heptasaccharide repeating unit, to produce a 1,2-linked glucan with occasional branching at position 3.…”

Section: Fig 2 Colonial Morphological Differences Between Strains Csupporting

confidence: 89%

Colonial Morphology of Burkholderia cepacia Complex Genomovar III: Implications in Exopolysaccharide Production, Pilus Expression, and Persistence in the Mouse

et al. 2003

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The purpose of this study was to determine the role of colonial morphology of Burkholderia cepacia complex (BCC) organisms in pathogenicity in a mouse model of pulmonary infection. BCC strain C1394 was rapidly cleared by leukopenic mice after intranasal challenge, whereas a spontaneous variant (C1394mp2) that was indistinguishable from the parent strain by genetic typing persisted in the lungs and differed in colonial morphology. The parent strain had a matte colonial phenotype, made scant exopolysaccharide (EPS), and was lightly piliated. The variant had a shiny phenotype, produced abundant EPS, and was heavily piliated. Matte to shiny colonial transformation was induced by growth at 42°C. Colonial morphology in the BCC strain variant was associated with persistence after pulmonary challenge and appeared to be correlated with the elaboration of putative virulence determinants.

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Section: Fig 2 Colonial Morphological Differences Between Strains Csupporting

confidence: 89%

Colonial Morphology of Burkholderia cepacia Complex Genomovar III: Implications in Exopolysaccharide Production, Pilus Expression, and Persistence in the Mouse

et al. 2003

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“…Also, rhizobacteria can fix nitrogen when involved in exopolysaccharides, as described by Serrato et al (2006) for the isolate B. tropica Ppe8. This feature may be widespread throughout the genus Burkholderia since other types of microbial exopolysaccharides have been studied in B. cepacia (Linker et al 2001). Here we found most of the Burkholderia spp.…”

Section: Discussionmentioning

confidence: 98%

Genetic diversity and plant-growth related features of Burkholderia spp. from sugarcane roots

Luvizotto

Marcon²,

Andreote

et al. 2010

World J Microbiol Biotechnol

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Brazil is the largest sugarcane producer in the world, mainly due to the development of different management strategies. Recently, microbial-plant related studies revealed that bacterial isolates belonging to the genus Burkholderia are mainly associated with this plant and are responsible for a range of physiological activity. In this study, we properly evaluate the physiological activity and genetic diversity of endophytic and rhizospheric Burkholderia spp. isolates from sugarcane roots grown in the field in Brazil. In total, 39 isolates previously identified as Burkholderia spp. were firstly evaluated for the capability to fix nitrogen, produce siderophores, solubilise inorganic phosphates, produce indole-acetic acid and inhibit sugarcane phytopathogens in vitro. These results revealed that all isolates present at least two positive evaluated activities. Furthermore, a phylogenetic study was carried out using 16S rRNA and gyrB genes revealing that most of the isolates were affiliated with the Burkholderia cepacia complex. Hence, a clear separation given by endophytic or rhizospheric niche occupation was not observed. These results presented an overview about Burkholderia spp. isolates from sugarcane roots and supply information about the physiological activity and genetic diversity of this genus, given direction for further studies related to achieve more sustainable cultivation of sugarcane.

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“…The mucoid material of BCC consists of several different polysaccharide species, the most common of which is called cepacian (19). This EPS species is composed of a branched acetylated heptasaccharide repeating unit and is produced by strains isolated in different parts of the world (20,(41)(42)(43). Cepacian appears to be a BCC specific EPS species; together with two other polysaccharides, dextran and polysaccharide-I (18), cepacian makes up the EPS used in this study (17).…”

Section: Discussionmentioning

confidence: 99%

Exopolysaccharides from Burkholderia cenocepacia Inhibit Neutrophil Chemotaxis and Scavenge Reactive Oxygen Species

Bylund

Burgess

Cescutti

et al. 2006

Journal of Biological Chemistry

135

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Bacteria belonging to the Burkholderia cepacia complex are important opportunistic pathogens in compromised hosts, particularly patients with cystic fibrosis or chronic granulomatous disease. Isolates of B. cepacia complex may produce large amounts of exopolysaccharides (EPS) that endow the bacteria with a mucoid phenotype and appear to facilitate bacterial persistence during infection. We showed that EPS from a clinical B. cenocepacia isolate interfered with the function of human neutrophils in vitro; it inhibited chemotaxis and production of reactive oxygen species (ROS), both essential components of innate neutrophil-mediated host defenses. These inhibitory effects were not due to cytotoxicity or interference with intracellular calcium signaling. EPS also inhibited enzymatic generation of ROS in cell-free systems, indicating that it scavenges these bactericidal products. B. cenocepacia EPS is structurally distinct from Pseudomonas aeruginosa alginate, yet they share the capacity to scavenge ROS and inhibit chemotaxis. These properties could explain why the two bacterial species resist clearance from the infected cystic fibrosis lung.

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The structure of a polysaccharide from infectious strains of Burkholderia cepacia

Cited by 98 publications

References 25 publications

Colonial Morphology of Burkholderia cepacia Complex Genomovar III: Implications in Exopolysaccharide Production, Pilus Expression, and Persistence in the Mouse

Colonial Morphology of Burkholderia cepacia Complex Genomovar III: Implications in Exopolysaccharide Production, Pilus Expression, and Persistence in the Mouse

Genetic diversity and plant-growth related features of Burkholderia spp. from sugarcane roots

Exopolysaccharides from Burkholderia cenocepacia Inhibit Neutrophil Chemotaxis and Scavenge Reactive Oxygen Species

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