The Structure, Function and Application of the Hammerhead Ribozyme

Birikh, Klara R.; Heaton, Paul A.; Eckstein, Fritz

doi:10.1111/j.1432-1033.1997.t01-3-00001.x

Cited by 269 publications

(182 citation statements)

References 165 publications

(158 reference statements)

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“…hammerhead motif ribozyme (Birikh et al, 1997); hairpin motif ribozyme (Fedor, 2000); hepatitis delta virus ribozyme (Been & Wickham, 1997); varkud satellite (VS) ribozyme (Collins, 2002)], and self-splicing RNAs [for example: group I introns (Schmidt et al, 1992) and group II introns (Jacquier, 1996)]. (c) Fig.…”

Section: Discussionmentioning

confidence: 99%

tRNA-dependent cleavage of the ColE1 plasmid-encoded RNA I

Wang

Yuan

et al. 2006

Microbiology

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Effects of tRNA Ala (UGC) and its derivative devoid of the 39-ACCA motif [tRNA Ala (UGC)DACCA] on the cleavage of the ColE1-like plasmid-derived RNA I were analysed in vivo and in vitro. In an aminoacid-starved relA mutant, in which uncharged tRNAs occur in large amounts, three products of specific cleavage of RNA I were observed, in contrast to an otherwise isogenic relA + host. Overexpression of tRNA Ala (UGC), which under such conditions occurs in Escherichia coli mostly in an uncharged form, induced RNA I cleavage and resulted in an increase in ColE1-like plasmid DNA copy number. Such effects were not observed during overexpression of the 39-ACCA-truncated tRNA Ala (UGC). Moreover, tRNA Ala (UGC), but not tRNA Ala (UGC)DACCA, caused RNA I cleavage in vitro in the presence of MgCl 2 . These results strongly suggest that tRNA-dependent RNA I cleavage occurs in ColE1-like plasmid-bearing E. coli, and demonstrate that tRNA Ala (UGC) participates in specific degradation of RNA I in vivo and in vitro. This reaction is dependent on the presence of the 39-ACCA motif of tRNA Ala (UGC).

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Section: Discussionmentioning

confidence: 99%

tRNA-dependent cleavage of the ColE1 plasmid-encoded RNA I

Wang

Yuan

et al. 2006

Microbiology

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“…[18][19][20][21] The antisense RNA technology utilizes ribonucleic acids that, like antisense oligonucleotides, bind by complementary interactions with a specific target mRNA (in this case forming RNA:RNA hybrids) and subsequently either block one of the several steps involved in the translation of a targeted protein or induced destruction of the target RNA most likely by activating an endonuclease activity following deamination of dsRNA by an adenosine deaminase. 22,23 Expression vectors producing antisense RNA have major advantages over the oligonucleotides in that, since vectors can synthesize the antisense RNA continuously inside the cell after a single administration, it would have a longer duration of action.…”

Section: Introductionmentioning

confidence: 99%

Viral vector producing antisense RNA restores myotonic dystrophy myoblast functions

Furling

Doucet²,

Ma³

et al. 2003

Gene Ther

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Myotonic dystrophy (DM1) is caused by the expansion of a trinucleotide repeat (CTG) located in the 3 0 untranslated region of the myotonic dystrophy protein kinase gene, for which currently there is no effective treatment. The data available suggest that misregulation of RNA homeostasis may play a major role in DM1 muscle pathogenesis. This indicates that the specific targeting of the mutant DMPK transcripts is essential to raise the rationale basis for the development of a specific gene therapy for DM1. We have produced a retrovirus which expresses a 149-bp antisense RNA complementary to the (CUG)13 repeats and to the 110-bp region following the repeats sequence to increase the specificity. This construct was introduced into human DM1 myoblasts, resulting in a preferential decrease in mutant DMPK transcripts, and effective restoration of human DM1 myoblast functions such as myoblast fusion and the uptake of glucose. It was previously shown that delay of muscle differentiation and insulin resistance in DM1 are associated with misregulation of CUGBP1 protein levels. The analysis of CUGBP1 levels and activity in DM1 cells expressing the antisense RNA indicated a correction of CUGBP1 expression in infected DM1 cells. We therefore show that current antisense RNA delivered in vitro using a retrovirus is not only capable of inhibiting mutant DMPK transcripts, but also can ameliorate dystrophic muscle pathology at the cellular levels. Gene Therapy (2003) 10, 795-802.

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“…Between the binding arms is a catalytic core where cleavage of the bound target RNA occurs. 19 For ribozymes to serve effectively as therapeutic agents, several factors are crucial. These include strategic design to optimize specificity and turnover, suitable delivery mechanisms and the enhancement of stability.…”

Section: Ribozyme's Potential In Cervical Cancer Controlmentioning

confidence: 99%