The structure and function of paramyxovirus glycoproteins

Analysis of pathotype-specific structural features and cleavage activation of Newcastle disease virus membrane glycoproteins using antipeptide antibodies

“…Protease(s) capable of cleavage at the motif of virulent strains appear to be distributed throughout a wider range of cells than those which can affect cleavage of the motif of less virulent strains (Nagai et al, 1976(Nagai et al, , 1979Klenk et al, 1977;Morrison, 1988;Morrison et al, 1985;Gorman et al, 1988Gorman et al, , 1990. Thus, virulent strains can spread more extensively through the host than less virulent strains and cause more severed disease as a consequence.…”

“…Variations in proteolytic activation of the membrane glycoprotein precursors of different strains offer the prospect of developing simpler, more precise and rapid assay systems for this purpose. Biosynthetic labelling of NDV proteins with radionuclides during propagation in cell culture has been shown to be useful for characterization of the susceptibilities of F 0 proteins to proteolytic activation and the nature of HN and HN 0 proteins (Nagai et al, 1976(Nagai et al, , 1979(Nagai et al, , 1980Klenk et al, 1977;. Antipeptide antibodies, targeted at regions either side of the cleavage activation sites of F 0 and HN 0 proteins, have been used to facilitate analysis of cell culture-propagated NDV isolates without the use of radioactivity (Gorman et al, 1992); however, even further improvement in the speed and ease of isolate characterization is desirable.…”

“…This variation in virulence is determined in part by variation in the susceptibilities of the fusion (F) protein biosynthetic precursors (F 0 proteins) of the different strains to proteolytic activation in different cell types (Nagai et al, 1976(Nagai et al, , 1979Klenk et al, 1977). Cleavage of this single chain F 0 protein results in formation of the active F protein which comprises disulphide-linked F~ and F1 polypeptides (Nagai et al, 1976;Scheid & Choppin, 1974.…”

Improvement of PVX/CMV CP expression tool for display of short foreign antigens

“…Electrophoretic mobility of the purified NDV virions probed with antibodies specific to CMV-HN revealed two protein bands with an apparent molecular weight of about 75 kDa and 63 kDa, presumably corresponding to the uncleaved HN 0 precursor and cleaved form of HN ( Fig. 2A) [8][9][10]. On the contrary, probing membranes with antibodies specific only to the CMV carrier, revealed a major band corresponding to the purified VLPs of CMV-HN (Fig.…”

Characterization of pathotype-specific epitopes of Newcastle disease virus fusion glycoproteins by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry and post-source decay sequencing

“…1) that would be present on virions isolated from biological specimens. 5,6,9,19,20 Hence deÐ-nition of the amino acid sequences of F2 polypeptides isolated from negatively reacting NDV strains is required to explain fully their negative reactivities and to be able to produce antisera to accommodate such sequences.…”