The structure and function of oxidized albumin in hemodialysis patients: Its role in elevated oxidative stress via neutrophil burst

Mera, Kiyomi; Anraku, Makoto; Kitamura, Kenichiro; Nakajou, Keisuke; Maruyama, Toru; Otagiri, Masaki

doi:10.1016/j.bbrc.2005.07.035

Cited by 126 publications

(120 citation statements)

References 27 publications

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confidence: 99%

“…AOPP are recognized as very good markers of oxidative damage to proteins and as indicators of disturbances in the oxidant/ antioxidant balance. 3,5,6 IMA is an albumin molecule modified by hypoxic conditions, which causes a change in its inherent affinity to bind transition metals such as Co 2+ , Ni 2+ , and Cu 2+ . The mechanism of formation of IMA may involve free radical release and damage to the N-terminus, although this remains to be proven in vivo.…”

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confidence: 99%

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Comparison of the usefulness of plasma levels of oxidatively modified forms of albumin in estimating kidney dysfunction in diabetic patients

Piwowar¹,

Knapik-Kordecka²,

Warwas³

2010

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Clin Invest Med 2010; 33 (2): E109-E116. AbstractPurpose: Advanced oxidation protein products (AOPP) and ischemia-modified albumin (IMA) are forms of oxidatidatively modified albumin and have recently been investigated as indicators of oxidative stress. They are increased in different disorders, including diabetes mellitus, as a result of hyperglycaemia, oxidative stress and hypoxia. The usefulness of the plasma levels of these two parameters in estimating kidney dysfunction in type 2 diabetic patients (T2DM) was compared in this study. Methods: Plasma levels of AOPP and IMA were determined spectrophotometrically in 218 individuals, 153 patients with T2DM and 65 healthy people.. The urinary albumin/creatinine ratio (UACR) was used as the reference to define the stage of kidney dysfunction by the assessment of the degree of albuminuria. Results: Receiver Operating Characteristic (ROC) curve analysis, likelihood ratio (LR), and Youden's index (J) revealed that AOPP and IMA had acceptable sensitivities and specificities in individuals with different degrees of albuminuria; however, AOPP had higher values of the area under the curve (AUC: 0.934) than IMA, as well as 100% sensitivity and 77.01% specificity for distinguishing patients with micro-and macroalbuminuria. Conclusions: Both AOPP and IMA may be helpful clinical markers for estimating kidney dysfunction, but AOPP is better able to identify diabetic patients with nephropathy. We suggest that AOPP is almost ideal for discriminating between T2DM patients with micro-and macroalbuminuria.In the plasma of diabetic patients, the albumin molecule is modified under conditions of chronic hypoxia, provoked mainly by the hyperglycaemia and oxidative stress (OS) existing in these patients. This leads to many changes in the structure and function of albumin including the formation of advanced oxidation protein products (AOPP) and ischemia-modified albumin (IMA). 1-4 AOPP were first detected in uremic and haemodialysed patients. They are formed during OS by the action of free radicals, mainly chloraminated oxidants (hypochlorous acid and chloramines) produced by myeloperoxidase in activated neutrophils. This leads to the formation of dityrosine residues and protein cross-linking, which results in abnormal albumin molecules in the plasma. AOPP are recognized as very good markers of oxidative damage to proteins and as indicators of disturbances in the oxidant/ antioxidant balance. 3,5,6 IMA is an albumin molecule modified by hypoxic conditions, which causes a ORIGINAL RESEARCH

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confidence: 99%

Comparison of the usefulness of plasma levels of oxidatively modified forms of albumin in estimating kidney dysfunction in diabetic patients

Piwowar¹,

Knapik-Kordecka²,

Warwas³

2010

CIM

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“…Given the fact that, in extracellular fluids, serum albumin plays a major antioxidant role (28,29), we expected that characterization of the oxidation status of serum albumin might provide useful information regarding the redox state of the human body, prompting us to examine the effect of olmesartan on the oxidation of albumin in vivo. Previously we reported that purified albumin from HD patients triggered oxidative bursts in neutrophils, and thus appeared to act as a true inflammatory mediator (30). Furthermore, the binding of ligands to albumins was found to decrease by oxidative modification of albumin (25,30).…”

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confidence: 89%

“…Previously we reported that purified albumin from HD patients triggered oxidative bursts in neutrophils, and thus appeared to act as a true inflammatory mediator (30). Furthermore, the binding of ligands to albumins was found to decrease by oxidative modification of albumin (25,30). Therefore, management of the oxidation status of serum albumin is an important issue in cases of CKD and medicine therapy.…”

Section: Discussionmentioning

confidence: 99%

Effect of Olmesartan on Oxidative Stress in Hemodialysis Patients

et al. 2007

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“…50) Diabetic complications exacerbate the condition by further increasing the amount of oxidized HSA molecules. 51,52) End-stage renal disease is associated with enhanced oxidative stress and may contribute to substantial cardiovascular complications in HD patients. The quality and integrity of HSA molecule in HD patients have been shown subtly altered and as a result HSA's biological properties are impaired.…”

Section: Fig 2 Oxidation Sites Of Human Serum Albumin (Hsa)mentioning

confidence: 99%

Pharmaceutically Important Pre- and Posttranslational Modifications on Human Serum Albumin

Otagiri

Chuang

2009

Biological & Pharmaceutical Bulletin

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Recombinant technology allows engineering and production of proteins with desirable properties. Human serum albumin has been developed with recombinant technology, and thus plays an increasing role as a drug carrier in the clinical setting. Genetic variations usually occur on the surface of the protein, and do not impose significant effects on the conformation of albumin. However, binding of fatty acids by genetic variants is affected according to the location of the mutation. Albumin undergoes three major posttranslational modifications, namely, oxidation, glycation, and S-nitrosylation. This review gives an account of the different posttranslational modifications that should be taken into consideration when designing albumin mutant analogues with desirable pharmaceutical properties.

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The structure and function of oxidized albumin in hemodialysis patients: Its role in elevated oxidative stress via neutrophil burst

Cited by 126 publications

References 27 publications

Comparison of the usefulness of plasma levels of oxidatively modified forms of albumin in estimating kidney dysfunction in diabetic patients

Comparison of the usefulness of plasma levels of oxidatively modified forms of albumin in estimating kidney dysfunction in diabetic patients

Effect of Olmesartan on Oxidative Stress in Hemodialysis Patients

Pharmaceutically Important Pre- and Posttranslational Modifications on Human Serum Albumin

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