The Structure and Composition of Bead-like Projections on Sitka Spruce Callus Cells Formed during Grafting and in Culture

Miller, H. N.

doi:10.1006/anbo.1993.1130

Cited by 27 publications

(19 citation statements)

References 24 publications

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“…3g). Adhesion between the cells from scion and rootstock is enhanced by binding material, composed of pectin, carbohydrate, protein and fatty acids [29]. Activation and induction of metabolic activities may contribute to nutrient transportation for the biosynthesis of the binding material [8].…”

Section: Discussionmentioning

confidence: 99%

Analysis of transcriptome in hickory (Carya cathayensis), and uncover the dynamics in the hormonal signaling pathway during graft process

et al. 2016

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BackgroundHickory (Carya cathayensis), a woody plant with high nutritional and economic value, is widely planted in China. Due to its long juvenile phase, grafting is a useful technique for large-scale cultivation of hickory. To reveal the molecular mechanism during the graft process, we sequenced the transcriptomes of graft union in hickory.ResultsIn our study, six RNA-seq libraries yielded a total of 83,676,860 clean short reads comprising 4.19 Gb of sequence data. A large number of differentially expressed genes (DEGs) at three time points during the graft process were identified. In detail, 777 DEGs in the 7 d vs 0 d (day after grafting) comparison were classified into 11 enriched Gene Ontology (GO) categories, and 262 DEGs in the 14 d vs 0 d comparison were classified into 15 enriched GO categories. Furthermore, an overview of the PPI network was constructed by these DEGs. In addition, 20 genes related to the auxin-and cytokinin-signaling pathways were identified, and some were validated by qRT-PCR analysis.ConclusionsOur comprehensive analysis provides basic information on the candidate genes and hormone signaling pathways involved in the graft process in hickory and other woody plants.Electronic supplementary materialThe online version of this article (doi:10.1186/s12864-016-3182-4) contains supplementary material, which is available to authorized users.

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Section: Discussionmentioning

confidence: 99%

Analysis of transcriptome in hickory (Carya cathayensis), and uncover the dynamics in the hormonal signaling pathway during graft process

et al. 2016

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“…The fusion between cells is one of the important properties of cell walls, dictated by wall cross‐links. The formation of inter‐polymer cross‐links, as occurs between cellulose and hemicellulose via H‐bonds, is necessary for the binding of juxtaposed cells between the stock and the scion (Fry 1986) and has been observed in projections of callus cells formed during grafting and in culture of Sitka spruce (Miller and Barnett 1993). As calcofluor stains cellulose (Heslop‐Harrison and Heslop‐Harrison 1981), weak stains may indicate that cellulose has been masked, as in old callus cells that are not involved in the development of the union between the two components of the graft.…”

Section: Discussionmentioning

confidence: 99%

“…These projections developed on the surface of callus cells may contain pectins and lipids (Juniper and Jefree 1983). Jefree et al (1989) also reported that the projections on the walls of Datura stramonium callus cells stained positively for lipids, although in Sitka spruce projections, while fatty acids were detected, no direct evidence for the presence of lipids was obtained (Miller and Barnett 1993).…”

Section: Presence Of Lipid and Phenolic Compounds In The Callus Cellsmentioning

confidence: 99%

“…Likewise, the content and nature of the cells implicated in the first step of graft formation can play an important role in triggering the responses that lead to the formation of a strong and successful union (Deloire and Hebant 1982, Macheix et al 1986, Errea 1998. Projections on the surfaces of callus cells in Strika spruce graft unions and in culture have been reported by Barnett and Weatherhead (1988) and Miller and Barnett (1993); these structures consist of a homogeneous matrix of a mixture of pectins, carbohydrate, protein and fatty acids. It has been suggested that the secretion of the warty material is a continuous process that has a binding or cementing function; the wide range of compounds that comprise them has a more active role in cell recognition and in the successful merging of tissues (Miller and Barnett 1993).…”

Section: Introductionmentioning

confidence: 99%

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Early detection of graft incompatibility in apricot (Prunus armeniaca) using in vitro techniques

Errea

Garay

Marín

2001

Physiologia Plantarum

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Graft compatibility has been studied in vitro using callus tissues of apricot (Prunus armeniaca) and different Prunus rootstocks to form scion/rootstock combinations with different degrees of graft compatibility. In these species, incompatibility is manifested by a breakdown of the trees at the union area that can occur some years after grafting. Here, the possibility of obtaining an early detection method to determine graft incompatibility is explored by callus fusion in vitro. The adhesion of the two callus partners, the development of the cells at the contact surface (cell arrangement, intensity of cell-wall staining), and the presence of lipid and phenolic compounds have been studied during the first 3 weeks after grafting in both compatible and incompatible combinations. Differences were observed at the second and the third week of callus co-culture in most of the characters determined, although these differences were present as early as the first week in the case of phenolic compounds. The behaviour of the grafts grown in vitro was correlated to that of the same combinations in the field, suggesting that callus fusion in vitro could be a possible and reliable method for an early detection of graft incompatibility in different Prunus combinations.

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“…These structures are thought to play a key role in graft establishment, taking part in the recognition and adhesion of the two sides of the graft (15)(16)(17)(18)(19)(20)(21). The increase in thickness is related to the deposition of wall polysaccharides by the protoplasts of nearby cells and the compacting of necrotic remains from the cut (20).…”

Section: Discussionmentioning

confidence: 99%

Histological changes associated with the graft union development in tomato

Frey

Acebes

Encina

et al. 2020

Preprint

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Background. Despite the importance of grafting in agriculture, particularly in horticultural crops such as tomato (Solanum lycopersicum L.), the structural changes that occur during the establishment of a graft are little understood. Using histochemical techniques, the present work examines the progression of the structure of the graft junction in tomato plants over time.Results. At 10 days after grafting, the cell walls of the scion and rootstock in the area of the graft junction were thicker than usual, and undifferentiated cells appeared associated with the pre-existing vascular tissue. New vascular tissue appeared as branches arising from the pre-existing vasculature, as vascular pockets dispersed within the callus, and as the result of the transdifferentiation of parenchyma cells. Areas showing vascular connections between the scion and rootstock were also seen. Adventitious roots appeared on the scion, arising from the pre-existing vasculature. At 20 days, a great deal of vascular tissue was visible, along with large areas showing vascular connection. At 210 days, vestiges of the changes undergone were still visible. However, no adventitious roots persisted. Conclusions. The area of the graft junction undergoes modifications essential for adequate physiological functioning of grafted plant. The cell walls of the adhesion line change during the process. Pre-existing vasculature plays an important role in the appearance of callus tissue, new vascular cells, and adventitious roots. A long time later the tissues maintain vestiges of graft union development.

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The Structure and Composition of Bead-like Projections on Sitka Spruce Callus Cells Formed during Grafting and in Culture

Cited by 27 publications

References 24 publications

Analysis of transcriptome in hickory (Carya cathayensis), and uncover the dynamics in the hormonal signaling pathway during graft process

Analysis of transcriptome in hickory (Carya cathayensis), and uncover the dynamics in the hormonal signaling pathway during graft process

Early detection of graft incompatibility in apricot (Prunus armeniaca) using in vitro techniques

Histological changes associated with the graft union development in tomato

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