The structural proteins of Newcastle disease virus

Reproduction of Paramyxoviruses

“…The initial reports on the virion proteins of NOV analyzed by polyacrylamide gel electrophoresis described the presence of three major proteins and a variable number of minor proteins (Evans and Kingsbury, 1969;Haslam et al, 1969;Bikel and Ouesberg, 1969). Subsequently, studies with SV5, NOV, and Sendai virus (Caliguiri et al, 1969;Mountcastle et al, 1971) indicated that there were 5-7 proteins in the virion, and possibly I or 2 additional minor proteins.…”

“…The previous reports that hemagglutinating and neuraminidase subunits of paramyxoviruses could be separated (Haslam et al, 1969;Iinuma et al, 1971;Brostrom et al, 1971) were not based on isolation of individual purified proteins, and were complicated by factors such as significant inactivation of neuraminidase in some cases and the probable formation of mixed aggregates of different sizes which differed in biological activities. The failure of the neuraminidase released from virions by a proteolytic enzyme (Maeno et al, 1970) to bind to or agglutinate red blood cells can probably now be explained by removal of the hydrophobic base of the large glycoprotein as discussed below, so that it was able to aggregate, was monovalent, and did not bind firmly to the cell surface.…”

Measles antibodies detected by platelet aggregation and gel precipitation in patients with subacute sclerosing panencephalitis

“…The number of lines in GP tests possibly reflects the number of measles-specific antigens in the host cells. The number of structural polypeptides in some paramyxoviruses, for example Newcastle disease virus seems to be limited to three (5). CALIGUIRI et al…”

Host induced modifications of Newcastle Disease Virus virion polypeptides

“…Both NDV (CAM) and NDV (BHK) were purified by the method of HASLA~ et al (5), omitting the ammonium sulphate precipitation step. All operations were carried out at 4 ° C. The infectivity and haemagglutinating activity per mg of protein were determined for the clarified virus used as starting material and for the material obtained from the peaks of infectious virus in the sucrose and potassium tartrate gradients.…”

“…NDV (CAM). Virus was grown on the chorioa]lantoic membrane (CAM) of 12 day old de-embryonated eggs as described by H~SLAS~ et al (5). Each membrane was infected with 6 ml standard medimn (3), containing 10 s.5 EIDs0 of virus and usually 40 ~xCi ~I-I-leueine (specific activity of 500--1000 mCi/mmol), or 10 ~Ci 140-protein hydrolysate (specific activity of >45 mCi/milliatom carbon), or 40 ixCi 3H-glucosamine (specific activity of 2> I0,000 mCi/mmol).…”