The Structural Proteins of Chick Embryo Lethal Orphan Virus (Fowl Adenovirus Type 1)

Abstract

SUMMARYChick embryo lethal orphan (CELO) virus (fowl adenovirus type 1) contains at least 14 structural proteins with polypeptide molecular weights ranging from 100K to about 6K. A nomenclature of the CELO virion polypeptides is presented and the molar proportion of each polypeptide has been estimated. The CELO virus pentons were specifically released from the virion by dialysis against borate-based calciummagnesium saline. The penton base (polypeptide III, mol. wt. 92K) and two fibres were separated, characterized and their polypeptides were correlated with their morphological positions in the virion. Peptide mapping suggested that the long fibre (polypeptide IV, tool. wt. 65K), and the short fibre (polypeptide VII, tool. wt. 44.5K) were not related in their primary sequences and are therefore probably encoded by separate genes. The time course of synthesis of the CELO virion polypeptides indicated that, like their mammalian adenovirus counterparts, they are synthesized late (after viral DNA replication).

Characterization of the structural proteins of hemorrhagic enteritis virus

“…They are smaller than those of Ad 2 (48k and 18.5k), more closely resenable those of FAV-1 (20k, 12k, and 9.5k) [29,33], and might be the two smallest polypeptides reported for group II avian adenoviruses [30]. The t2k and 9.5k proteins of FAV-1 might be the counterparts of the 12.5k and 9.5k proteins of HEV, respectively.…”

DNA-binding Proteins of Chick Embryo Lethal Orphan Virus: Lack of Complementation between Early Proteins of Avian and Human Adenoviruses

“…Methods for growth, radioactive labelling, purification and titration of viruses, and for polyacrylamide gel electrophoresis (PAGE) have been described (Laver et al, 1971 ;Younghusband & Bellett, 1971: Braithwaite et aL, 1983Li et aL, 1984).…”

“…[35S]Methionine-labelled bands were cut from polyacrylamide gels, digested with chymotrypsin, and the resulting peptides mapped as described (Li et al, , 1984.…”

“…The dl group samples [35S]Methionine-containing peptide maps of the CELO virus DBP. Bands corresponding to the four CELO virus DBP (74K, 64K, 56K and 52K) were cut from a gel, and chymotryptic maps prepared as described previously (Li et aL, , 1984. The central inset is a schematic drawing of the main peptides shared by the four CELO virus DBP.…”

Pathogenic and pathological characteristic of new type gosling viral enteritis first observed in China

“…After scanned and analyzed by automatic gel image-forming and analyzing system, the molecular mass and relative percentage of each structural polypept ide were obtained ( Table 1). The fact that VP 4 , VP 7 , VP 8 , VP 9 and VP 14 were the main structural polypeptides and made up about 90.2622% of the gross protein revealed that NGVEV fulfilled the criteria of adenovirus to great extent [17,[26][27][28] . From the relationship between DNA base number and the molecular mass of the encoded protein, it is known that NGVEV structural protein with an overall molecular weight of 71 300 D needed DNA of approximately 19.3 kb to code for.…”