The strong anti-glioblastoma capacity of the plasma-stimulated lysine-rich medium

Yan, Dayun; Nourmohammadi, Niki; Talbot, Annie; Sherman, Jonathan H.

doi:10.1088/0022-3727/49/27/274001

Cited by 28 publications

(30 citation statements)

References 26 publications

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“…Compared to traditional CAP, PAM can be stored for a long time and used without the CAP device ( Adachi et al, 2015 ; Judée et al, 2016 ). DMEM plus 10% FBS was used as the source solution of PAM in our study, without the addition of antibiotics or β-ME ( Yan et al, 2014 ; Boehm et al, 2016 ; Yan et al, 2016a ; Yan et al, 2016b ). In fact, some additives such as ferrum and lysine were proved to enhance the anti-tumor ability of PAM ( Yan et al, 2016a ; Adachi et al, 2016 ).…”

Section: Discussionmentioning

confidence: 99%

Gene expression profiling and functional analysis reveals that p53 pathway-related gene expression is highly activated in cancer cells treated by cold atmospheric plasma-activated medium

Shi

Feng

et al. 2017

PeerJ

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BackgroundCold atmospheric-pressure plasma (CAP) has been considered a promising strategy for anti-cancer treatment. Traditionally, CAP was employed to kill cancer cells or tumor tissues by direct irradiation. However, CAP has some disadvantages such as infiltration capacity and storage convenience. Recently, plasma-activated medium (PAM) was used as an alternative strategy to treat cancer cells or tumors. The novel PAM approach has potential as an anti-cancer therapy.ObjectiveTo reveal the global activation of signaling pathways in oral cancer cells induced by PAM.MethodsOral squamous cell line SCC15 were treated by PAM and gene expression profiles were evaluated by using RNA-seq. Functional analyses were employed to reveal the global responses of SCC15 cells with PAM stimulation. QRT-PCR and Western blot were carried out to validate the expression levels of selected genes.ResultsMore than 6G clean data per sample were obtained in PAM-treated SCC15 cells. A total of 934 differentially expressed genes (DEGs) were identified and GO analysis implicated the deep involvement of biological process. KEGG mapping further clustered 40 pathways, revealing that “p53 pathway” was significantly enriched. SCC15 cells were commonly used as a p53-null cell line. Therefore, the enriched p53 pathway-related genes in our analysis might be activated by other stimulators, in a p53-independent manner. Gene set enrichment analysis (GSEA) was also performed to evaluate changes at the gene-sets level. The results demonstrated not only the high engagement of “p53 pathway” but also the involvement of novel pathways such as hypoxia pathway.ConclusionsThe present study elucidates the transcriptomic changes of PAM treated SCC15 cells, containing highly enriched DEGs involved in “p53 pathway”. Our analysis in this work not only provides genomic resources for future studies but also gives novel insights to uncover the molecular mechanism of PAM stimulation.

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Section: Discussionmentioning

confidence: 99%

Gene expression profiling and functional analysis reveals that p53 pathway-related gene expression is highly activated in cancer cells treated by cold atmospheric plasma-activated medium

Shi

Feng

et al. 2017

PeerJ

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“…In a recent study, Girard and colleagues demonstrated the synergistic effect of H2O2 and nitrite in killing normal as well as cancerous cells and conclude a similar mechanism of action for the effects of a He plasma jet [16]. The cytotoxic effects of plasma-activated medium depend on the amino acids present, on scavengers such as pyruvate and can be modulated through the addition of FBS [1,[28][29][30].…”

Section: Introductionmentioning

confidence: 97%

Hydrogen Peroxide and Beyond-the Potential of High-voltage Plasma-activated Liquids Against Cancerous Cells

Boehm¹,

Curtin²,

Cullen³

et al. 2018

ACAMC

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“…NBP induces the production of intracellular ROS and activates DNA damage signaling, including p53 expression [15,16]. These reactive species have a key function in the induction of apoptosis in cancer cells specifically exposed to NBP [5,[17][18][19] or indirectly treated by plasma-activated water or materials [20][21][22][23][24][25][26][27]. Nonthermal atmospheric pressure plasma jets (NBP-Js) have gained attention in the biomedical field [28][29][30][31][32].…”

Section: Introductionmentioning

confidence: 99%

Non-Thermal Atmospheric Pressure Bio-Compatible Plasma Stimulates Apoptosis via p38/MAPK Mechanism in U87 Malignant Glioblastoma

Akter

Jangra

Choi

et al. 2020

Cancers

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Nonthermal plasma is a promising novel therapy for the alteration of biological and clinical functions of cells and tissues, including apoptosis and inhibition of tumor progression. This therapy generates reactive oxygen and nitrogen species (RONS), which play a major role in anticancer effects. Previous research has verified that plasma jets can selectively induce apoptosis in various cancer cells, suggesting that it could be a potentially effective novel therapy in combination with or as an alternative to conventional therapeutic methods. In this study, we determined the effects of nonthermal air soft plasma jets on a U87 MG brain cancer cell line, including the dose- and time-dependent effects and the physicochemical and biological correlation between the RONS cascade and p38/mitogen-activated protein kinase (MAPK) signaling pathway, which contribute to apoptosis. The results indicated that soft plasma jets efficiently inhibit cell proliferation and induce apoptosis in U87 MG cells but have minimal effects on astrocytes. These findings revealed that soft plasma jets produce a potent cytotoxic effect via the initiation of cell cycle arrest and apoptosis. The production of reactive oxygen species (ROS) in cells was tested, and an intracellular ROS scavenger, N-acetyl cysteine (NAC), was examined. Our results suggested that soft plasma jets could potentially be used as an effective approach for anticancer therapy.

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The strong anti-glioblastoma capacity of the plasma-stimulated lysine-rich medium

Cited by 28 publications

References 26 publications

Gene expression profiling and functional analysis reveals that p53 pathway-related gene expression is highly activated in cancer cells treated by cold atmospheric plasma-activated medium

Gene expression profiling and functional analysis reveals that p53 pathway-related gene expression is highly activated in cancer cells treated by cold atmospheric plasma-activated medium

Hydrogen Peroxide and Beyond-the Potential of High-voltage Plasma-activated Liquids Against Cancerous Cells

Non-Thermal Atmospheric Pressure Bio-Compatible Plasma Stimulates Apoptosis via p38/MAPK Mechanism in U87 Malignant Glioblastoma

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