The stress response mechanisms of biofilm formation under sub-lethal photocatalysis

Chen, Min; Cai, Yiwei; Li, Guiying; Zhao, Huijun; An, Taicheng

doi:10.1016/j.apcatb.2022.121200

Cited by 36 publications

(9 citation statements)

References 46 publications

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“…However, it was reported that titanium dioxide was effective for sterilization of the drug-resistant gram-negative bacteria recently. 42 , 43 Though the titanium dioxide is not medical supplies, we may expect it as a new porin activating agent.…”

Section: Discussionmentioning

confidence: 99%

Cefmetazole Resistance Mechanism for Escherichia Coli Including ESBL-Producing Strains

Ito¹,

Kawamura²,

Sato³

et al. 2022

IDR

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Purpose Cefmetazole (CMZ), a cephamycin antibiotic, is primarily used as a definitive therapy for Extended Spectrum β-Lactamase (ESBL)-producing Escherichia coli infections. However, the mechanism of CMZ resistance in E. coli is still unknown. To elucidate the resistance mechanism and to determine combined drugs for prevention of resistance acquisition. Methods Clinical isolates of 14 ESBL-producing E. coli and non-producing 12 isolates were used in in vitro testing of CMZ resistance acquisition. After 10-day of CMZ exposure (1st subculture), these strains were incubated in an antibacterial-free medium for 14-day. These strains were again exposed to CMZ for 10-day (2nd subculture) and confirmed for changes in MIC. For each strain detected after 1st subculture, each mRNA expression level of porin, chromosomal ampC , and drug-efflux pump was measured using real-time RT-PCR. Relebactam (REL) has the potency to recover antimicrobial activity against carbapenem-resistant Enterobacterales that has porin deficiency. REL was added to the CMZ dilution series, and MIC changes and those of porin were confirmed. Results Of these 26 strains, 15 strains (57.7%) acquired resistance after 1st subculture, but after passage culture on the antibacterial-free medium, 11 strains recovered susceptibility. These 11 strains showed resistance after 2nd subculture. The expression levels of ompF and ompC were significantly decreased in these strains ( P <0.05). When REL was added, all strains suppressed resistance acquisition after 1st subculture. The mechanism was the activation of ompF . Conclusion Our results showed that the mRNA expression levels of genes encoding porin were decreased in the strains that acquired resistance due to CMZ exposure, and that ompF and ompC in particular were thought to be involved in the acquisition of resistance. The CMZ acquisition of resistance was also suppressed by the concomitant use of REL and actually suppressed the decrease in mRNA expression in ompF . It was confirmed that porin reactivated by REL.

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Section: Discussionmentioning

confidence: 99%

Cefmetazole Resistance Mechanism for Escherichia Coli Including ESBL-Producing Strains

Ito¹,

Kawamura²,

Sato³

et al. 2022

IDR

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“…Silversol-pre-exposed S. aureus culture had 1.30-fold higher EPS production (Figure 3B). Enhanced EPS secretion may be taken as a response to the stress induced in bacterial population by Silversol, as EPS production is known to be a part of bacterial stress-response (Wang et al, 2020; Chen et al, 2022).…”

Section: Resultsmentioning

confidence: 99%

Silversol^®(a colloidal nanosilver formulation) inhibits growth of antibiotic-resistantStaphylococcus aureusby disrupting its physiology in multiple ways

Thakkar,

Gajera,

Godse

et al. 2024

Preprint

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Antibacterial effect of a colloidal nanosilver formulation Silversol was investigated against an antibioticresistant strain of Staphylococcus aureus. Lower concentrations of the test formulation exerted bacteriostatic and, its higher concentrations exerted bactericidal effect against this pathogen. Silversol at sub-lethal concentration was found to disturb multiple physiological traits of S. aureus such as growth, antibiotic susceptibility, membrane permeability, efflux, protein synthesis and export, biofilm and exopolysaccharide production, etc. Transcriptome data revealed the genes coding for transcriptional regulators, efflux machinery, transferases, beta-lactam resistance, oxidoreductases, metal homeostasis, virulence factors, and arginine biosynthesis to get expressed differently under influence of the test formulation. Genes (argG and argH) involved in arginine biosynthesis emerged among the major targets of Silversol in S. aureus.

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“…[80] In our study, the csgG (gene-C7A06_RS20170), cpsB (gene-C7A06_RS12050), mdtQ (gene-C7A06_RS11590), and wzc (gene-C7A06_RS11995) genes responsible for the maturation of biofilms were all upregulated , indicating that the EPGNs can promote the bacterial biofilm formation, which was similar to antibiotics at sublethal concentrations or sublethal photocatalysis that can also induce biofilm formation. [81,82] The yegX (gene-C7A06_RS11790) gene encoding the lysozyme was upregulated (with a log 2 (fold change) value of 6.08), indicating that EPGN treatment enhanced the production of lysozyme, which may exert an inhibitory effect toward the bacterial cell wall formation, thus inducing bacterial cell lysis. Besides, the high upregulation of lysozyme-encoding genes will help to produce lysozyme in the future.…”

Section: Transcriptomic Analysis Of E Coli After Epgn Treatment and D...mentioning

confidence: 99%

Expand and Sensitize: Guanidine‐Functionalized Exopolysaccharide Nanoparticles Cause Bacterial Cell Expansion and Antibiotic Sensitization

Li,

Jia,

Seidi

et al. 2023

Adv Funct Materials

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Conventional antibacterial agents and mechanisms are frequently observed to be ineffective due to the evolution of bacteria to the strains with stronger antibiotic resistance, and hence developing alternative antibacterial materials and mechanisms is urgently needed. Here, guanidine‐functionalized exopolysaccharide (EPS) nanoparticles (termed EPGNs) with durable antibacterial and antibiofilm activities are developed. Very interestingly, the EPGNs obtained by the reaction of EPS, epichlorohydrin, and polyhexamethylene guanidine hydrochloride exhibit an unconventional antibacterial mechanism, i.e., they can induce substantial bacterial cell expansion by upregulating the SulA and DicB proteins that are responsible for cell division inhibition, along with the increase of reactive oxygen species production, bacterial cell surface disruption, and bacterial ribosomal RNA degradation. The transcriptome analysis reveals that EPGNs can hinder cell motility, induce loss of cell integrity, decrease the resistance of bacteria to oxidative stress, and finally lead to cell death. Moreover, EPGNs can effectively accelerate the bacteria‐infected wound healing. This work provides the first example that nanomaterials can cause bacterial cell expansion by affecting intracellular structures and inhibiting cell division, and it may inspire other researchers to investigate the effect of antibacterial materials on the change of bacterial volume and design unconventional antibacterial materials/strategies.

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The stress response mechanisms of biofilm formation under sub-lethal photocatalysis

Cited by 36 publications

References 46 publications

Cefmetazole Resistance Mechanism for Escherichia Coli Including ESBL-Producing Strains

Cefmetazole Resistance Mechanism for Escherichia Coli Including ESBL-Producing Strains

Silversol^®(a colloidal nanosilver formulation) inhibits growth of antibiotic-resistantStaphylococcus aureusby disrupting its physiology in multiple ways

Expand and Sensitize: Guanidine‐Functionalized Exopolysaccharide Nanoparticles Cause Bacterial Cell Expansion and Antibiotic Sensitization

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The stress response mechanisms of biofilm formation under sub-lethal photocatalysis

Cited by 36 publications

References 46 publications

Cefmetazole Resistance Mechanism for Escherichia Coli Including ESBL-Producing Strains

Cefmetazole Resistance Mechanism for Escherichia Coli Including ESBL-Producing Strains

Silversol®(a colloidal nanosilver formulation) inhibits growth of antibiotic-resistantStaphylococcus aureusby disrupting its physiology in multiple ways

Expand and Sensitize: Guanidine‐Functionalized Exopolysaccharide Nanoparticles Cause Bacterial Cell Expansion and Antibiotic Sensitization

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Product

Resources

About

Silversol^®(a colloidal nanosilver formulation) inhibits growth of antibiotic-resistantStaphylococcus aureusby disrupting its physiology in multiple ways