The Stress of Suicide: Temporal and Spatial Expression of Putative Heat Shock Protein 70 Protect the Cells from Heat Injury in Wheat (Triticum aestivum)

Abstract

Heat stress adversely affects growth, development, and yield of winter wheat (Triticum aestivum). Plants have, however, evolved mechanisms to adapt to such conditions mainly by the expression of stress-associated chaperones, the heat shock proteins (HSPs), for modulating the tolerance level. Here, we report cloning of cytosolic putative HSP70 of 1678 bp from a thermotolerant cultivar (C306) of wheat (T. aestivum). A BLASTn search showed maximum homology with the predicted HSP70 protein reported from Hordeum vulgare (accession no AK354795.1). In silico characterization showed the presence of a nucleotide-binding domain of the sugar kinase/HSP70/ actin superfamily in the sequence. Putative HSP70 showed temporal and spatial variations in the expression under heat stress (HS). We observed the abundance of HSP70 protein, H 2 O 2 , proline, and guaiacol peroxidase activity during the seed-hardening stage under HS; accumulation was, however, higher in the thermotolerant C306 than in thermosusceptible HD2329 cultivar. A gradual decrease in cell membrane stability (CMS) and an increase in total antioxidant capacity (TAC) were observed in both the cultivars at the different stages of growth. The expression of HSP70 showed a negative correlation with CMS and a positive correlation with TAC under HS; changes were less pronounced in C306 than in HD2329 at all the stages of growth studied. HSP70 seems to play diverse roles associated with thermotolerance, and partially protect wheat from terminal HS. Being the important member of family of the HSPs, HSP70 needs to be studied in detail, to be used for developing climate-smart wheat crops, through genetic engineering/breeding approaches.

A furoviral replicase recruits host HSP70 to membranes for viral RNA replication

“…We therefore searched the NCBI transcriptional database of wheat and found 27 expressed sequence tags (ESTs). These ESTs were assembled into four contigs of HSP70-like genes, which were almost identical to those in previous reports, including AF0059931014, AF07496921, GQ28038215, and KJ02755122. We then designed primer pairs for quantitative RT-PCR (qRT-PCR) and characterized the expression profile of these genes in Triticum aestivum with or without CWMV infection.…”

Knockout of SlMAPK3 enhances tolerance to heat stress involving ROS homeostasis in tomato plants

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Overcoming Reproductive Compromise Under Heat Stress in Wheat: Physiological and Genetic Regulation, and Breeding Strategy

“…The expression of HSP70 could decrease membrane stability and enhance total antioxidant capacity under heat stress. Kumar et al, 2016 normal temperature conditions due to accelerated development.…”