The Stress-Chip: A microfluidic platform for stress analysis in<i>Caenorhabditis elegans</i>

Banse, Stephen A.; Blue, Benjamin W.; Robinson, Kristin J.; Jarrett, Cody M.; Phillips, Patrick C.

doi:10.1101/285163

Cited by 6 publications

(21 citation statements)

References 43 publications

(57 reference statements)

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“…A commercialized version of the NemaLife microfluidics chip combined with a benchtop machine that regulates fluid flow through the chip and enables time-laps image recording and analysis has been developed ( ). Similarly, a device called the stress-chip combines crawling and swimming areas [ 268 ]. It should be mentioned that, to the best of our knowledge, no large drug screens in microfluidic devices have been conducted so far.…”

Section: Automated Phenotyping and Lifespan Devicesmentioning

confidence: 99%

“…Bacterial food concentrations and environmental conditions, such as the presence of metabolites and waste products from the worms and bacteria, change over time in agar plate-based assays. Periodic transfer of the worms to new plates can minimize changes in the environment but the transfer also introduces other problems such as loss of worms or physical damage [ 268 ]. In contrast, microfluidic systems that use continuous flow of fresh bacterial solution through the worm chambers keep food concentrations and environmental conditions constant [ 246 ].…”

Section: Automated Phenotyping and Lifespan Devicesmentioning

confidence: 99%

“…The scanner used in the lifespan machine uses fluorescent bulbs, which produce more heat than LEDs. However, switching to scanners that use LEDs can reduce this problem [ 268 ]. The software for the lifespan machine is freely available and recently an improved version was released in beta ( ).…”

Section: Automated Phenotyping and Lifespan Devicesmentioning

confidence: 99%

“…It should also be noted that microfluidics devices have been used for stress survival tests in C. elegans [ 268 , 273 ]. For example, a microfluidics device was used to test the effect of polydatin and (+/−)- α -Tocopherol on lifespan, both in unstressed and Cu 2+ -stressed conditions, validating this device for its use in drug screenings [ 273 ].…”

Section: Automated Phenotyping and Lifespan Devicesmentioning

confidence: 99%

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Phenotypic Screening in C. elegans as a Tool for the Discovery of New Geroprotective Drugs

Bulterijs

Braeckman

2020

Pharmaceuticals

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Population aging is one of the largest challenges of the 21st century. As more people live to advanced ages, the prevalence of age-related diseases and disabilities will increase placing an ever larger burden on our healthcare system. A potential solution to this conundrum is to develop treatments that prevent, delay or reduce the severity of age-related diseases by decreasing the rate of the aging process. This ambition has been accomplished in model organisms through dietary, genetic and pharmacological interventions. The pharmacological approaches hold the greatest opportunity for successful translation to the clinic. The discovery of such pharmacological interventions in aging requires high-throughput screening strategies. However, the majority of screens performed for geroprotective drugs in C. elegans so far are rather low throughput. Therefore, the development of high-throughput screening strategies is of utmost importance.

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Section: Automated Phenotyping and Lifespan Devicesmentioning

confidence: 99%

Section: Automated Phenotyping and Lifespan Devicesmentioning

confidence: 99%

Section: Automated Phenotyping and Lifespan Devicesmentioning

confidence: 99%

Section: Automated Phenotyping and Lifespan Devicesmentioning

confidence: 99%

See 2 more Smart Citations

Phenotypic Screening in C. elegans as a Tool for the Discovery of New Geroprotective Drugs

Bulterijs

Braeckman

2020

Pharmaceuticals

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“…Generally, the imaging-based approaches fall into two categories. First, several microfluidic devices have been developed that allow worms to be kept in liquid media and assayed by light microscopy-based systems (Xian et al 2013;Banse et al 2019a;Saberi-Bosari et al 2018). Microfluidic systems have some benefits over traditional solid culture.…”

Section: Introductionmentioning

confidence: 99%

WormBot, an open-source robotics platform for survival and behavior analysis in C. elegans

et al. 2019

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Caenorhabditis elegans is a popular organism for aging research owing to its highly conserved molecular pathways, short lifespan, small size, and extensive genetic and reverse genetic resources. Here we describe the WormBot, an open-source robotic image capture platform capable of conducting 144 parallel C. elegans survival and behavioral phenotyping experiments. The WormBot uses standard 12-well tissue culture plates suitable for solid agar media and is built from commercially available robotics hardware. The WormBot is controlled by a web-based interface allowing control and monitoring of experiments from any internet connected device. The standard WormBot hardware features the ability to take both time-lapse bright field images and real-time video micrographs, allowing investigators to measure lifespan, as well as heathspan metrics as worms age. The open-source nature of the hardware and software will allow for users to extend the platform and implement new software and hardware features. This extensibility, coupled with the low cost and simplicity of the system, allows the automation of C. elegans survival analysis even in small laboratory settings with modest budgets.

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NemaLife chip: a micropillar-based microfluidic culture device optimized for aging studies in crawling C. elegans

Rahman

Edwards

Birze

et al. 2020

Sci Rep

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In this study, we report a microfluidic device for the whole-life culture of the nematode Caenorhabditis elegans that allows the scoring of animal survival and health measures. This device referred to as the NemaLife chip features: (1) an optimized micropillar arena in which animals can crawl, (2) sieve channels that separate progeny and prevent the loss of adults from the arena during culture maintenance, and (3) ports that allow rapid accessibility for feeding the adult-only population and introducing reagents as needed. The pillar arena geometry was optimized to accommodate the growing body size during culture and emulate the body gait and locomotion of animals reared on agar. Likewise, feeding protocols were optimized to recapitulate longevity outcomes typical of standard plate growth. Key benefits of the NemaLife Chip include eliminating the need to perform repeated manual transfers of adults during survival assays, negating the need for progeny-blocking chemical interventions, and avoiding the swim-induced stress across lifespan in animals reared in liquid. We also show that the culture of animals in pillar-less microfluidic chambers reduces lifespan and introduces physiological stress by increasing the occurrence of age-related vulval integrity disorder. We validated our pillar-based device with longevity analyses of classical aging mutants (daf-2, age-1, eat-2, and daf-16) and animals subjected to RNAi knockdown of age-related genes (age-1 and daf-16). We also showed that healthspan measures such as pharyngeal pumping and tap-induced stimulated reversals can be scored across the lifespan in the NemaLife chip. Overall, the capacity to generate reliable lifespan and physiological data underscores the potential of the NemaLife chip to accelerate healthspan and lifespan investigations in C. elegans.

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The Stress-Chip: A microfluidic platform for stress analysis inCaenorhabditis elegans

Abstract: 1 8

Cited by 6 publications

References 43 publications

Phenotypic Screening in C. elegans as a Tool for the Discovery of New Geroprotective Drugs

Phenotypic Screening in C. elegans as a Tool for the Discovery of New Geroprotective Drugs

WormBot, an open-source robotics platform for survival and behavior analysis in C. elegans

NemaLife chip: a micropillar-based microfluidic culture device optimized for aging studies in crawling C. elegans

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