The streptozotocin-high fat diet induced diabetic mouse model exhibits severe skin damage and alterations in local lipid mediators

Abstract

BackgroundType 2 diabetes (T2D) can go undiagnosed for years, leading to a stage where produces complications such as delayed skin wound healing. Animal models have been developed in the last decades to study the pathological progression in this disease. Streptozotocin (STZ), that has a selective pharmacological toxicity toward pancreatic β cells, in addition to high fat diet has been widely used to induce diabetes however no evidence has shown its effects on the skin integrity.MethodsEighteen C57BL/6J male mice, were divided in 3 groups; the first was fed with chow diet and the second was kept on a high fat diet and the third injected with STZ intraperitoneal for 5 days consecutively before starting the diet protocol with high fat. Mice were maintained 5 weeks in total.ResultsWe show that animals treated with STZ-high fat diet exhibit skin injuries without significant alterations on basal insulin and triglycerides, compared to the control. The skin from these animals presents higher levels of oxidative stress, lower levels of adhesion proteins and alterations in lipid mediators, effects that are not produced by the high fat diet itself.ConclusionOur results suggest that this in vivo model represents a relevant approach for studying skin damage induced by diabetes.

Myricitrin Ameliorates Hyperglycemia, Glucose Intolerance, Hepatic Steatosis, and Inflammation in High-Fat Diet/Streptozotocin-Induced Diabetic Mice

“…Hyperglycemia, insulin resistance, and β-cell failure are key characteristics of type 2 diabetes. In accordance with previous findings, compared to non-DM mice, our HFD/STZ-treated mice showed higher levels of fasting blood glucose and insulin resistance as demonstrated by the impaired glucose tolerance in an IPGTT test [18][19][20]. In addition, plasma insulin levels and the numbers of pancreatic insulin-secreting β-cells were markedly decreased in the HFD/STZ-treated DM mice compared to these values in non-DM mice.…”

Diet‐induced rodent models of obesity‐related metabolic disorders—A guide to a translational perspective

“…via single or multiple injections of varying dosages, ranging from 25 to 45 mg/kg BW in rats and 40 to 100 mg/kg BW in mice. [185][186][187][188][189] As an example, Mansor et al 183 191 At the end of the experimental protocol, the animals displayed frank hyperglycaemia (>300 mg/dl), marked dyslipidaemia, hepatic fibrosis and pancreatic β-cell dysfunction with subsequent insulinopenia. 191 Interestingly, in addition to signs of cardiovascular injury, these animals developed microvascular angiopathies, namely, nephropathy, retinopathy and behavioural signs of peripheral neuropathy.…”

“…192 These observations have been corroborated by other reports. 184,186,187 Finally, it should be emphasized that diet-induced regimens com- (Table 3). Furthermore, even in genetic animal models of NASH, diet acts as a secondary trigger for disease progression.…”

Dengue in Taiwan: Pointing the finger at Aedes aegypti

“…In this brief communication, Leguina-Ruzzi et al. [24] demonstrate that mice treated with streptozotocin, which selectively kills pancreatic beta cells, and fed a high fat diet show the typical skin lesions observed in diabetes, making this model particularly useful for studying this complication.…”