The strawberry transcription factor FaRAV1 positively regulates anthocyanin accumulation by activation of <i>FaMYB10</i> and anthocyanin pathway genes

Zhang, Zuying; Shi, Yanna; Ma, Yu-Chen; Yang, Xin; Yin, Xin; Zhang, Yuanyuan; Xiao, Yuwei; Liu, Wenli; Li, Yunduan; Li, Shaojia; Liu, Xiaofen; Grierson, Donald; Allan, Andrew C.; Jiang, Guihua; Chen, Kunsong

doi:10.1111/pbi.13382

Cited by 99 publications

(78 citation statements)

References 83 publications

(103 reference statements)

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“…In this study, the expression pattern of CitRAV1 transcript accumulation suggested it may also be involved in the process of fruit ripening as well as leaf senescence, which was consistent with previous observations (Woo et al ., 2010). Recently, RAV1 from strawberry was reported to regulate anthocyanin accumulation by directly activating transcription of FaMYB10 and other genes, excluding CHI (Zhang et al ., 2020). In the present study, we showed that although CitRAV1 cannot directly bind to the promoter of CitCHIL1 either, it can form homologous dimers or oligomers and combine with CitERF33 to form transcriptional complexes (Figure 6), which has not been previously described.…”

Section: Discussionmentioning

confidence: 99%

Three AP2/ERF family members modulate flavonoid synthesis by regulating type IV chalcone isomerase in citrus

Zhao

Liu

Gong

et al. 2020

Plant Biotechnology Journal

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112

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Summary Flavanones and flavones are excellent source of bioactive compounds but the molecular basis of their highly efficient production remains elusive. Chalcone isomerase (CHI) family proteins play essential roles in flavonoid biosynthesis but little are known about the transcription factors controlling their gene expression. Here, we identified a type IV CHI (designated as CitCHIL1) from citrus which enhances the accumulation of citrus flavanones and flavones (CFLs). CitCHIL1 participates in a CFL biosynthetic metabolon and assists the cyclization of naringenin chalcone to (2S)‐naringenin, which leads to the efficient influx of substrates to chalcone synthase (CHS) and improves the catalytic efficiency of CHS. Overexpressing CitCHIL1 in Citrus and Arabidopsis significantly increased flavonoid content and RNA interference‐induced silencing of CitCHIL1 in citrus led to a 43% reduction in CFL content. Three AP2/ERF transcription factors were identified as positive regulators of the CitCHIL1 expression. Of these, two dehydration‐responsive element binding (DREB) proteins, CitERF32 and CitERF33, activated the transcription by directly binding to the CGCCGC motif in the promoter, while CitRAV1 (RAV: related to ABI3/VP1) formed a transcription complex with CitERF33 that strongly enhanced the activation efficiency and flavonoid accumulation. These results not only illustrate the specific function that CitCHIL1 executes in CFL biosynthesis but also reveal a new DREB‐RAV transcriptional complex regulating flavonoid production.

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Section: Discussionmentioning

confidence: 99%

Three AP2/ERF family members modulate flavonoid synthesis by regulating type IV chalcone isomerase in citrus

Zhao

Liu

Gong

et al. 2020

Plant Biotechnology Journal

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112

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“…7’ after fading was significantly lower than that in ‘Xinqihong’ at the late coloring stage, which indicates that the expression of PbRAV6 was closely related to the synthesis of anthocyanins. In strawberry ( Fragaria ananassa ), FaRAV1 was shown to promote anthocyanin accumulation by directly binding to the promoter of various genes in the anthocyanin biosynthesis pathway and increasing their expression including FaMYB10 (4.0 fold), chalcone synthase (CHS; 1.53-fold), flavanone hydroxylase (F3H; 1.95-fold), dihydroflavonol 4-reductase (DFR; 3.6-fold), and 5,3-O-glucosyltransferase (GT)1 (2.3-fold) [ 40 ]. In pear, PbRAV6 may have a similar function, which requires further investigation.…”

Section: Discussionmentioning

confidence: 99%

Genome-Wide Analysis of RAV Transcription Factors and Functional Characterization of Anthocyanin-Biosynthesis-Related RAV Genes in Pear

Liu

Deng

Liang

et al. 2021

IJMS

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Related to ABSCISIC ACID INSENSITIVE3/VIVIPAROUS1 (ABI3/VP1, RAV), transcription factors (TFs) belonging to the APETALA2/ETHYLENE RESPONSIVE FACTOR (AP2/ERF) TF family play critical roles in plant growth, development, and responses to abiotic and biotic stress. In this study, 11 novel RAV TFs were identified in pear (Pyrus bretschneideri Rehd). A phylogenetic analysis revealed that the TFs clustered into three groups with 10 conserved motifs, some of which were group- or subgroup-specific, implying that they are important for the functions of the RAVs in these clades. RAVs in Pyrus and Malus were closely related, and the former showed a collinear relationship. Analysis of their expression patterns in different tissues and at various growth stages and their responses to abiotic and biotic stress suggested that PbRAV6 and PbRAV7 play important roles in drought stress and salt stress, respectively. We investigated the function of RAVs in pear peel coloration using two red pear varieties with different color patterns and applying data from transcriptome analyses. We found that PbRAV6 participates in the regulation of pericarp color. These findings provide insight into a new TF family in pear and a basis for further studies on the response to drought stress and fruit coloration in this commercially important crop.

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“…In strawberry, FaMYB9/FaMYB11, FabHLH3, and FaTTG1 from the octoploid Fragaria ananassa form an MBW complex specifically activating proanthocyanidins (Schaart et al, 2013), whereas in a transient assay FvMYB10 and FvbHLH33 from the diploid Fragaria vesca were found to activate the anthocyanin-specific DFR and UFGT promoters (Lin- Wang et al, 2014). Recently, a RAV (related to ABI3/viviparous 1) transcription factor, FaRAV1, has been demonstrated to stimulate anthocyanin accumulation both by up-regulating FaMYB10 and by directly binding to promoters of anthocyanin biosynthetic genes (Zhang et al, 2020a). In pear, an MBW complex has not been identified, but PyMYB114 and PybHLH3 were found to interact with PyERF3 in red pear fruit.…”

Section: Activators and Repressors Of Anthocyanin Biosynthesis In Fruits Vegetables And Other Cerealsmentioning

confidence: 99%

Anthocyanins: From Mechanisms of Regulation in Plants to Health Benefits in Foods

et al. 2021

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Anthocyanins represent the major red, purple, and blue pigments in many flowers, fruits, vegetables, and cereals. They are also recognized as important health-promoting components in the human diet with protective effects against many chronic diseases, including cardiovascular diseases, obesity, and cancer. Anthocyanin biosynthesis has been studied extensively, and both biosynthetic and key regulatory genes have been isolated in many plant species. Here, we will provide an overview of recent progress in understanding the anthocyanin biosynthetic pathway in plants, focusing on the transcription factors controlling activation or repression of anthocyanin accumulation in cereals and fruits of different plant species, with special emphasis on the differences in molecular mechanisms between monocot and dicot plants. Recently, new insight into the transcriptional regulation of the anthocyanin biosynthesis, including positive and negative feedback control as well as epigenetic and post-translational regulation of MYB-bHLH-WD40 complexes, has been gained. We will consider how knowledge of regulatory mechanisms has helped to produce anthocyanin-enriched foods through conventional breeding and metabolic engineering. Additionally, we will briefly discuss the biological activities of anthocyanins as components of the human diet and recent findings demonstrating the important health benefits of anthocyanin-rich foods against chronic diseases.

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The strawberry transcription factor FaRAV1 positively regulates anthocyanin accumulation by activation of FaMYB10 and anthocyanin pathway genes

Cited by 99 publications

References 83 publications

Three AP2/ERF family members modulate flavonoid synthesis by regulating type IV chalcone isomerase in citrus

Three AP2/ERF family members modulate flavonoid synthesis by regulating type IV chalcone isomerase in citrus

Genome-Wide Analysis of RAV Transcription Factors and Functional Characterization of Anthocyanin-Biosynthesis-Related RAV Genes in Pear

Anthocyanins: From Mechanisms of Regulation in Plants to Health Benefits in Foods

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