The strategy of group embryo culture based on pronuclear pattern on blastocyst development: a two center analysis

Restelli, Liliana; Paffoni, Alessio; Corti, Laura; Rabellotti, Elisa; Mangiarini, Alice; Viganò, Paola; Somigliana, Edgardo; Papaleo, Enrico

doi:10.1007/s10815-014-0350-9

Cited by 22 publications

(26 citation statements)

References 17 publications

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“…In animal models, small culture volume as well embryo grouping have been proposed as two different strategies to improve embryo developmental potential [2,4,5,7,8,10,18,19,30] since autocrine and paracrine factors of embryonic origin are likely to mimick in vivo conditions [1,4,5,7,9,13]. In a recent review, Reed and coauthors [7] describe the impact of group versus individual cultures on embryo development.…”

Section: Discussionmentioning

confidence: 99%

“…Studies concerning embryo physiology and metabolism have highlighted the importance of autocrine and paracrine growth factors affecting embryo development in vivo [1][2][3][4][5][6][7][8][9][10]. However, recent data have shown that preimplantation embryos promote their own development in vitro also in absence of paracrine factors secreted by the female reproductive tract [4,6,7,11,12].…”

Section: Introductionmentioning

confidence: 99%

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Improved blastocyst formation with reduced culture volume: comparison of three different culture conditions on 1128 sibling human zygotes

Minasi

Fabozzi

Casciani

et al. 2014

J Assist Reprod Genet

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Purpose The aim of the present randomized, comparative study was to evaluate the effect of reduced culture volumes on sibling human embryo development. Methods Firstly, sibling injected oocytes obtained from 88 out of 165 consenting couples undergoing infertility treatment were cultured either in large (35 μl) or in small drops (15 μl) of culture medium. Secondly, sibling injected oocytes from 77 couples were cultured either in large (35 μl) or in mini drops (7 μl). Embryo quality on day-2 and day-3 and blastocyst formation rate on day-5 were evaluated. Results No statistically significant difference in terms of embryo quality was detected comparing embryos cultured either in large (35 μl) or small (15 μl) drops until blastocyst stage. Similarly, no difference appeared between large (35 μl) or mini (7 μl) drops until day-3, however a significantly higher blastocyst formation rate was observed in mini (7 μl) drops on day-5. Conclusions Reduced culture volume seems not to influence early embryo development but a reduction of medium appears to positively affect blastocyst development. This supports the hypothesis that the pre-implantation embryo produces autocrine factors which exert a positive effect on embryo development when culture is performed in a reduced volume.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Improved blastocyst formation with reduced culture volume: comparison of three different culture conditions on 1128 sibling human zygotes

Minasi

Fabozzi

Casciani

et al. 2014

J Assist Reprod Genet

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show abstract

“…For ICSI, denudation of the cumulus oophorus was performed as previously described [23–24]. Inseminated or injected oocyte were grouped cultured in microdrops of equilibrated HSA-supplemented Fertilization or Serum Substitute Supplement (SSS, Irvine, CA, USA)-supplemented Cleavage medium (Sage In-Vitro Fertilization, Inc. Trumbull, CT, USA) under oil respectively.…”

Section: Methodsmentioning

confidence: 99%

Top quality blastocyst formation rates in relation to progesterone levels on the day of oocyte maturation in GnRH antagonist IVF/ICSI cycles

et al. 2017

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Cycles with progesterone elevation during controlled ovarian stimulation (COS) for IVF/ICSI are commonly managed with a “freeze-all” strategy, due to a well-recognized detrimental effect of high progesterone levels on endometrial receptivity. However, also a detrimental effect of elevated progesterone on day-3 embryo quality has recently been found with regards to top quality embryo formation rate. Because blastocyst culture and cryopreservation are largely adopted, we deemed relevant to determine whether this detrimental effect is also seen on blastocyst quality on day 5–6. This issue was investigated through a large two-center retrospective study including 986 GnRH antagonist IVF/ICSI cycles and using top quality blastocyst formation rate as the main outcome. Results showed that on multivariate analysis sperm motility (p<0.01) and progesterone levels at ovulation triggering (p = 0.01) were the only two variables that significantly predicted top quality blastocyst formation rate after adjusting for relevant factors including female age, BMI, basal AMH and total dose of FSH used for COS. More specifically, progesterone levels at induction showed an inverse relation with top quality blastocyst formation (correlation coefficient B = -1.08, 95% CI -1.9 to -0.02) and ROC curve analysis identified P level >1.49 ng/ml as the best cut-off for identification of patients at risk for the absence of top quality blastocysts (AUC 0.55, p<0.01). Our study is the first to investigate the top quality blastocyst formation rate in relation to progesterone levels in IVF/ICSI cycles, showing that increasing progesterone is associated with lower rates of top quality blastocyst. Hence, the advantages of prolonging COS to maximize the number of collected oocytes might eventually be hindered by a decrease in top quality blastocysts available for transfer, if increasing progesterone levels are observed. This observation extends the results of two recent studies focused on day-3 embryos and deserves further research.

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“…In the study by Ebner et al [5], grouped culture was compared to individual culture with or without possible medium exchange, and a higher blastulation rate was observed in the grouped culture (55.8 versus 40.8 and 45.2 %, respectively, per normally fertilized zygote). An equally low blastocyst formation rate (∼40 %) was observed in the study of Restelli et al [13] where random versus Bdefinite^(based on the zygote type) grouping was compared. The study of RebollarLazaro and Matson [12] showed a significantly higher rate of usable blastocysts when grouped culture was performed until day 3 (51.3 %) compared to single culture to day 5 (46.5 %).…”

Section: Discussionmentioning

confidence: 83%

“…Despite the long history of IVF, the best culture system is yet to be elected and the majority of the labs use different methods to culture their embryos. At present, three major embryo culture systems are used: (i) grouped culture, with three to five human zygotes/embryos cultured together [22,5,12,13], (ii) individual culture, where every embryo is cultured in an individual droplet [22,10,12], and (iii) individual culture with medium exchange between embryo droplets, where although the embryos are cultured individually, the presence of small communicating orifices in the walls allow the passage of medium [5]. Besides the culture system, the choice of the culture medium is also important and may be either: (i) single media, in which the zygote/embryo is cultured from day 0/1 until day 5 or (ii) sequential media, composed of both a cleavage and a blastocyst medium, in which a consecutive medium change is performed on day 3 of embryo development.…”

Section: Introductionmentioning

confidence: 99%

Reduced blastocyst formation in reduced culture volume

Munck¹,

Santos‐Ribeiro

Mateizel³

et al. 2015

J Assist Reprod Genet

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Purpose The aim of this prospective sibling oocyte study was to evaluate whether reduced culture volume improves blastocyst formation. Methods Twenty-three patients with extended embryo culture until day 5 were selected for the study. After injection, 345 sibling oocytes were individually cultured in either 25 or 7 μl droplets of Origio cleavage medium under oil. On day 3 of development, embryos were transferred to droplets with the corresponding volume of Origio blastocyst culture medium. Fertilization and embryo quality on day 3 and day 5/6 were evaluated. Results No statistically significant difference (p=0.326) in fertilization rate was observed (81.3 versus 83.0 %). There was no significant difference in terms of the number of excellent and good-quality embryos obtained on day 3 between both groups (p=0.655). Embryo culture in 25 μl droplets led to more embryos with a higher cell number when compared to 7 μl culture (p=0.024). On day 3, 132 and 131 embryos were considered for further culture until day 5/6. Blastulation rates were significantly higher in the 25 μl group (75.0 versus 61.6 %; p=0.017) and significantly more day 5 embryos with excellent and good quality were found in this group (54.5 versus 40.5 %; p=0.026). Finally, the utilization rates expressed per mature oocyte (41.4 versus 29.8 %; p=0.043), per fertilized oocyte (50.7 versus 36.6 %; p=0.023), and per day 3 embryo undergoing extended culture to day 5/6 (54.5 versus 39.7 %; p=0.019) were all significantly higher in the 25 μl group. Conclusion Reduced culture volume (7 μl) negatively impacts early development by reducing the cell number on day 3 and both blastocyst formation and quality.

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The strategy of group embryo culture based on pronuclear pattern on blastocyst development: a two center analysis

Abstract: Two strategies of group culture gave similar results in terms of blastulation rate but the random grouping of zygotes improves pregnancy and implantation rates in IVF-cycles.

Cited by 22 publications

References 17 publications

Improved blastocyst formation with reduced culture volume: comparison of three different culture conditions on 1128 sibling human zygotes

Improved blastocyst formation with reduced culture volume: comparison of three different culture conditions on 1128 sibling human zygotes

Top quality blastocyst formation rates in relation to progesterone levels on the day of oocyte maturation in GnRH antagonist IVF/ICSI cycles

Reduced blastocyst formation in reduced culture volume

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