The story of a toxic protein, 1888–1992

Am, Pappenheimer

doi:10.1002/pro.5560020218

Cited by 30 publications

(13 citation statements)

References 2 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Tg rats were gifted by Dr Wei Zhang (School of Science, Sun Yan-Sen University, Guangzhou, China). Rats were maintained under standard conditions with free access to feed and water and a 12-h light/dark cycle (lights on at 07:00), as previously outlined (21). Diphtheria toxin (DT; D0564; Sigma-Aldrich; Merck Millipore, Darmstadt, Germany) was used to stimulate kidney injury in the rats.…”

Section: Translationally-controlled Tumor Protein Activates the Transmentioning

confidence: 99%

“…Diphtheria toxin (DT; D0564; Sigma-Aldrich; Merck Millipore, Darmstadt, Germany) was used to stimulate kidney injury in the rats. The minimum lethal dose of DT for humans was reported to be 100 ng/kg body weight (21,22). Based upon the standard protocol (23), 10 ng/kg DT is efficient at causing kidney injury in Tg rats.…”

Section: Translationally-controlled Tumor Protein Activates the Transmentioning

confidence: 99%

See 1 more Smart Citation

Translationally-controlled tumor protein activates the transcription of Oct-4 in kidney-derived stem cells

Jing

Mei

2016

Experimental and Therapeutic Medicine

View full text Add to dashboard Cite

Abstract. The molecula r mechanisms underlying translationally-controlled tumor protein (TCTP) in the activation of octamer-binding transcription factor 4 (Oct-4) in kidney-derived stem cells have not been characterized. The aim of the present study was to identify the transcriptional activation of Oct-4 by TCTP in kidney-derived stem cells. Homology-directed repair cDNA inserted into Fisher 344 transgenic (Tg) rats and the mouse strain 129/Svj were used for the experiments. Diphtheria toxin (DT; 10 ng/kg) injected into the Tg rats created the kidney injury, which was rapidly restored by the activation of kidney-derived stem cells. Kidney-derived stem cells were isolated from the DT-injured Tg rats using cell culture techniques. The co-expression of Oct-4 and TCTP were observed in the isolated kidney-derived stem cells. Immunoblotting and reverse transcription-polymerase chain reaction analysis of TCTP null mutant (TCTP -/ -

show abstract

Section: Translationally-controlled Tumor Protein Activates the Transmentioning

confidence: 99%

Section: Translationally-controlled Tumor Protein Activates the Transmentioning

confidence: 99%

Translationally-controlled tumor protein activates the transcription of Oct-4 in kidney-derived stem cells

Jing

Mei

2016

Experimental and Therapeutic Medicine

View full text Add to dashboard Cite

show abstract

“…This announcement came a month after I arrived at Harvard in September 1972. Embarking on my first immunology course, I then learnt of Porter, papain, and the prize from Alwin M Pappenheimer Jr. ‘Pap’, as he was known to all, had gained his PhD at Harvard in 1932, and had gone on to win several awards for his work on Diphtheria toxin's mechanism of action .…”

Section: Solubilization and Purification Of Transplantation Antigensmentioning

confidence: 99%

Molecular definition of the transplantation antigens

Parham

2018

The FEBS Journal

View full text Add to dashboard Cite

In the first half of the 20th century, the major histocompatibility complex (MHC) of the laboratory mouse, the H-2 complex, was defined by a combination of serology and genetics. In the second half of the 20th century, its human counterpart, the human leukocyte antigen (HLA) complex was similarly defined and shown to mediate rejection of allogeneic kidney grafts. The clinical relevance of the transplantation antigens created the field of transplant immunology, which aimed to reduce graft rejection by HLA matching of transplant donors and recipients, and to use immunosuppressive drugs to prevent and treat rejection. Because tissue transplantation is not a natural phenomenon, the relevance of the MHC for immunology and immune defense against microbial pathogens was frequently questioned. In the 1970s, the general observation that cytotoxic T-cell responses to viral infection required recognition of both a viral antigen and a transplantation antigen argued for the immunological importance of the MHC. Proving this point was not achieved until close to the end of the 20th century. This required detailed biochemical and structural analysis of the transplantation antigens, the viral antigens, and the T-cell receptors that recognized them. This century of research culminated in 1996 with the three-dimensional crystallographic structure of the complex of these three components. In this complex is MAC, the very first HLA antigen to be detected and now more formally known as HLA-A*02:01.

show abstract

“…C. diphtheriae produces diphtheria toxin (DT) and a pseudomembrane adherent to the tonsils, pharynx, and/or nose. The local and systemic manifestations of diphtheria are mainly related to the action of DT, which is the most studied virulence factor of this species [4] [5] [6] [7]. DT is a polypeptide of 535 amino acids with a molecular weight of approximately 58.3 kDa, which is proteolytically cleaved after secretion into two fragments with distinct activities.…”

Section: Introductionmentioning

confidence: 99%

<i>Mycobacterium bovis</i> BCG as a Delivery System for the <i>dtb</i> Gene Antigen from Diphtheria Toxin

Nascimento¹,

Dellagostin²,

Matos³

et al. 2017

AJMB

View full text Add to dashboard Cite

Diphtheria is a fulminant bacterial disease caused by toxigenic strains of Corynebacterium diphtheriae whose local and systemic manifestations are due to the action of the diphtheria toxin (DT). The vaccine which is used to prevent diphtheria worldwide is a toxoid obtained by detoxifying DT. Although associated with high efficacy in the prevention of disease, the current anti-diphtheria vaccine, one of the components of DTP (diphtheria, tetanus and pertussis triple vaccine), may present post vaccination effects such as toxicity and reactogenicity resulting from the presence of contaminants in the vaccine that originated during the process of production and/or detoxification. Therefore, strategies to develop a less toxic and at the same time economically viable vaccine alternatives are needed to improve existing vaccines in use worldwide. In this study, the Moreau substrain of BCG which is used in Brazil as a live vaccine against human tuberculosis was genetically modified to carry and express the gene encoding for the diphtheria toxin fragment B (DTB). As such, the DNA sequence encoding the dtb gene was cloned into the pUS977 shuttle vector for cytoplasmic expression and successfully introduced into BCG cells by electroporation. Mice immunized with recombinant BCG expressing DTB showed seroconversion with the detection of specific antibodies against DTB. Also, rBCGs stably expressing DTB persisted up to 60 days in the absence of selective pressure in mice and cell viability did not change significantly during the period tested. Finally, immune sera from BALB/c mice vaccinated with rBCGpUS977dtb PW8 were preliminarily tested for their capacity of neutralizing the diphtheria toxin in the Vero Cells assay.How to cite this paper: Nascimento, D.V.,

show abstract

The story of a toxic protein, 1888–1992

Cited by 30 publications

References 2 publications

Translationally-controlled tumor protein activates the transcription of Oct-4 in kidney-derived stem cells

Translationally-controlled tumor protein activates the transcription of Oct-4 in kidney-derived stem cells

Molecular definition of the transplantation antigens

<i>Mycobacterium bovis</i> BCG as a Delivery System for the <i>dtb</i> Gene Antigen from Diphtheria Toxin

Contact Info

Product

Resources

About

The story of a toxic protein, 1888–1992

Cited by 30 publications

References 2 publications

Translationally-controlled tumor protein activates the transcription of Oct-4 in kidney-derived stem cells

Translationally-controlled tumor protein activates the transcription of Oct-4 in kidney-derived stem cells

Molecular definition of the transplantation antigens

&lt;i&gt;Mycobacterium bovis&lt;/i&gt; BCG as a Delivery System for the &lt;i&gt;dtb&lt;/i&gt; Gene Antigen from Diphtheria Toxin

Contact Info

Product

Resources

About

<i>Mycobacterium bovis</i> BCG as a Delivery System for the <i>dtb</i> Gene Antigen from Diphtheria Toxin