The stolbur phytoplasma antigenic membrane protein gene stamp is submitted to diversifying positive selection

Fabre, Anne Lise; Danet, Jean-Luc; Foissac, Xavier

doi:10.1016/j.gene.2010.10.012

Cited by 90 publications

(145 citation statements)

References 31 publications

Supporting

Mentioning

134

Contrasting

Unclassified

Order By: Relevance

“…P. solani' strains and allowed us to identify 11 distinct genotypes with a maximum variability of 4.9 %. Among the identified stamp genotypes, six were identical to previously published reference strains: 19-25, SB5, Rqg50, Vv24, GGY and STOL (Fabre et al 2011;Cvrković et al 2014), and five were unique and designated as M1, M2, M3, M4 and M5 (Fig. 2, Table 2).…”

Section: Enantiocephalus Cornutus 1 -mentioning

confidence: 51%

“…P. solani', was amplified by nested PCR with StampF/R0 followed by StampF1/R1 primers, with PCR conditions according to Fabre et al (2011). The resulting amplicons were sequenced by Macrogen Inc. by using the forward primer only and the sequences are deposited in the GenBank database under accession numbers KP337309-23.…”

Section: Stamp Genementioning

confidence: 99%

“…The stamp sequences were edited with FinchTV v.1.4.0 and compared with reference stamp strains (Fabre et al 2011;Johannesen et al 2012;Aryan et al 2014;Cvrković et al 2014;Kostadinovska et al 2014).…”

Section: Stamp Genementioning

confidence: 99%

“…Three of them, namely M1, M2 and M3, have unique sequences (Fabre et al 2011;Johannesen et al 2012;Aryan et al 2014;Cvrković et al 2014;Kostadinovska et al 2014). The strains of each stamp genotype detected in this study are designated in bold letters.…”

Section: Enantiocephalus Cornutus 1 -mentioning

confidence: 99%

“…Phytoplasma solani' through a multilocus sequencing approach (Cimerman et al 2009;Fabre et al 2011;Pacifico et al 2009). These two genes have higher sequence variability than the tuf gene, and thus they are more widely used in epidemiological studies of BN phytoplasmas (Johannesen et al 2012;Aryan et al 2014;Cvrković et al 2014;Kostadinovska et al 2014).…”

mentioning

confidence: 99%

See 4 more Smart Citations

The molecular epidemiology of bois noir grapevine yellows caused by ‘Candidatus Phytoplasma solani’ in the Republic of Macedonia

et al. 2015

View full text Add to dashboard Cite

Bois noir (BN), which is induced by 'Candidatus Phytoplasma solani' ('Ca. P. solani'), is an important grapevine yellows disease that causes severe damage in viticultural regions throughout the EuroMediterranean basin. An epidemiological survey to determine potential insect vectors and the primary reservoir plants of BN phytoplasma in Macedonian vineyards was undertaken between 2012 and 2013 in the southeastern part of the country. A study on the species diversity from the suborder Auchenorrhycha revealed the prevalence of the principal vector of 'Ca. P. solani', which is the planthopper Hyalesthes obsoletus. Reptalus panzeri, which is the second-most documented BN vector, was not recorded in Macedonian vineyards. Three leafhopper species, namely Psammotettix alienus, Artianus manderstjernii and Euscelis incisus, were also widespread in the BNaffected vineyards, but only H. obsoletus tested positive for 'Ca. P. solani'. Molecular characterizations were performed by the sequencing and/or RFLP typing of tuf, vmp1 and stamp genes, and the results were used to gain detailed insight into the molecular diversity of the 'Ca. P. solani' strains associated with grapevines, tentative reservoir plants (Urtica dioica and Convolvulus arvensis) and the H. obsoletus associated with these plants. Among the 91 'Ca. P. solani' strains detected in diverse plant and insect hosts, three tuf, five vmp1 and 11 distinct stamp genotypes were identified. Twelve comprehensive genotypes of 'Ca. P. solani' were detected according to the tuf/vmp1/stamp genotyping. The highest diversity of genotypes was detected among the strains from H. obsoletus individuals associated with U. dioica, of which the most frequent genotype was tuf-ab/V18/M1 (43 %). The tuf-b/V2-TA/STOL comprehensive genotype was found in 33 % of naturally infected grapevines. Two 'Ca. P. solani' genotypes were associated with U. dioica, namely (i) tuf-ab/V18/M1 (60 %) and tuf-a/V3/M4 (40 %), and only one genotype (tuf-b/V2-TA/Rqg50) was associated with C. arvensis.

show abstract

Section: Enantiocephalus Cornutus 1 -mentioning

confidence: 51%

Section: Stamp Genementioning

confidence: 99%

Section: Stamp Genementioning

confidence: 99%

Section: Enantiocephalus Cornutus 1 -mentioning

confidence: 99%

mentioning

confidence: 99%

See 3 more Smart Citations

The molecular epidemiology of bois noir grapevine yellows caused by ‘Candidatus Phytoplasma solani’ in the Republic of Macedonia

et al. 2015

View full text Add to dashboard Cite

show abstract

Tuf and secY PCR Amplification and Genotyping of Phytoplasmas

Foissac

Danet

Malembic-Maher

et al. 2012

Methods in Molecular Biology

View full text Add to dashboard Cite

Tuf and secY genotyping techniques have been developed to distinguish phytoplasma strains. Tuf polymerase chain reaction sequence analyses are available for phytoplasma taxonomic groups 16SrI, 16SrV, 16SrXII-A, and XII-B. In addition to their use to confirm the taxonomic status of phytoplasma strains, they allow the spread of phytoplasma strains in host plants and insect vectors to be traced. SecY is more variable than tuf and is therefore more discriminatory than tuf, but secY and tuf phylogenies show congruence.

show abstract

Preparation of Phytoplasma Membrane Recombinant Proteins

Galetto

Siampour

Marzachı̀

2012

Methods in Molecular Biology

View full text Add to dashboard Cite

The study of phytoplasma membrane protein interactions with host cell proteins is crucial to understand the life cycle of these unculturable microorganisms within their hosts. A step-by-step protocol for the heterologous expression of phytoplasma membrane proteins in Escherichia coli is described, together with a procedure to purify a suitable quantity of fusion antigen for application in the study of phytoplasma-host interactions.

show abstract

The stolbur phytoplasma antigenic membrane protein gene stamp is submitted to diversifying positive selection

Cited by 90 publications

References 31 publications

The molecular epidemiology of bois noir grapevine yellows caused by ‘Candidatus Phytoplasma solani’ in the Republic of Macedonia

The molecular epidemiology of bois noir grapevine yellows caused by ‘Candidatus Phytoplasma solani’ in the Republic of Macedonia

Tuf and secY PCR Amplification and Genotyping of Phytoplasmas

Preparation of Phytoplasma Membrane Recombinant Proteins

Contact Info

Product

Resources

About