The Status of Parathyroid Hormone Measurements in Humans

Fischer, Jan A.; Binswanger, U; Born, Walter; Ma, Dambacher; Tschopp, Fritz A.

doi:10.1007/978-1-4612-5284-9_113

Cited by 1 publication

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“…It appears from published reports that serum N-terminal PTH levels are useful in discriminating between healthy subjects and hyperparathyroid patients (6,7,8,3,9,10) in venous catheterization studies for localization of parathyroid tissue (11), hypercalcaemic secondary hyperparathyroidism, chronic renal failure, and in the investigation of the pharmacokinetics of exogenously administered hPTH (1-34) (12,13). However, from the view point of reliability and sensitivity the current N-terminal PTH immunoassays based on polyclonal antibodies are far from adeguate for this purpose (14).…”

Section: Introductionmentioning

confidence: 96%

Monoclonal Antibodies to Human Parathyroid Hormone (1-34) and Their Use in the Immunocytochemical Detection of Parathyroid Tumours

Mohamed¹,

Hubbard²,

Ah-Sing³

et al. 1987

Hybridoma

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Monoclonal antibodies against the biologically active N-terminal fragment of human parathyroid hormone, hPTH (1-34), were produced. The procedure included the use of novel secondary immunization in vitro of mouse spleen cell cultures. Dissociated spleen cells from primary immunized Balb/c mice, were cultured for five days in the presence of thymocyte conditioned media (TCM) and synthetic hPTH (1-34). Contrary to previous findings by other workers, in our hands Balb/c mice responded well. Following immunization the spleen cells were fused with NSl myeloma cells and cultured for eleven days before screening for antibody. Using an enzyme linked immunosorbent assay (ELISA) a number of positive clones were detected. Positive cells were cloned by limiting dilution and fifteen specific monoclonal hybridomas were produced. The immunoglobulin class of the different monoclonal antibodies was found to be IgGl. The immunocytochemical reaction was tested with chief cell carcinoma tissue and found to be clearly positive.

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