The state of pro- and antioxidant systems in rats with DMH-induced colon carcinogenesis on the background of extracorporeal detoxification

Abstract

Aim: Cancer is one of the leading causes of death in the world. The aim of this research was to study the indices of pro- and antioxidant systems in rats with dimethylhydrazine (DMH)-induced colon carcinogenesis on the background of the enterosorbent AUT-M use. Materials and methods: The study was performed on 70 white male rats weighing 200–250 g. Adenocarcinoma of the colon was simulated by subcutaneous injection of the DMH (Sigma-Aldrich Chemie, Japan) at a dose of 7.2 mg/kg once a week during 7 months. Enterosorbent AUT-M was administered intragastrically daily for 21 days after simulation of carcinogenesis at a dose of 1 ml of suspension per 100 g of animal body weight. The state of the pro- and antioxidant systems was studied by the content of oxidative modification of proteins products (OMP), the activity of superoxide dismutase (SOD), catalase (CAT), contents of ceruloplasmin (CP) and reduced glutathione (GSH). Results: It was found that DMH-induced colon carcinogenesis in rats is accompanied by disorders in the antioxidant defense system and activation of free radical oxidation processes. Enterosorbent AUT-M provides a significant reduction in the content of OMP370 and OMP430 in both blood serum and liver homogenate of rats. Moreover, the use of enterosorbent AUT-M demonstrated a significant increase in the activity of SOD, CAT, content of GSH and a decrease in CP content in investigated tissues. Conclusion: The use of enterosorbent AUT-М demonstrated prominent potential suppression for oxidative stress and positive effect on antioxidant defense system in rats with DMH-induced colon carcinogenesis.

Evaluation of membrane-destructive processes in rats with induced carcinogenesis of the colon using the cytostatic vincristine

“…It is known that the development of a malignant process is accompanied by impaired liver function, in particular, changes in the permeability and structure of hepatocytes [5,6].…”

“…Adenocarcinoma of the colon in rats was simulated by subcutaneous administration of the carcinogen 1,2-dimethylhydrazine (DMH) (Sigma-Aldrich Chemie, Japan), pre-diluted with saline in the interscapular region at a dose of 7.2 mg / kg [2,5,6].…”

AUT-M enterosorbent stabilizes glutathione system in vincristine-treated rats with dimethylhydrazine-induced colon cancer

“…The system glutathione peroxidase -glutathione reductase -reduced glutathione plays the main role in the destruction of hydroperoxides formed during the activation of free radical processes [4,5,18]. GSH performs the function of the main modulator of the enzymatic redox system of glutathione, as well as the activity of glutathionedependent enzymes.…”

Вплив Молекулярного Водню На Окиснювальну Модифікацію Протеїнів При Колоректальному Раку В Експерименті

“…Окиснювальний стрес (дисбаланс прооксидантно/антиоксидантного гомеостазу) призводить до підвищення рівнів протеїнових карбонільних груп [5,[8][9][10]. Карбонільні групи (альдегіди та кетони) утворюються на бічних ланцюгах протеїну (особливо Pro, Arg, Lys і Thr), коли вони окиснюються.…”