The state of cytokine reactivity in patients treated with ozonobaroterapy at the complex treatment of festering diseases and wounds of limbs

Abstract

Вивчено вміст TNF-α, IL-2, IL-8 в крові у 162 хворих з гнійними ранами. Встановлений позитивний зв'язок між концентрацією прозапальних цитокінів та об'ємом і площею ран, виявлено зниження рівня цитокінів при хронізації загоєння. Застосування озонобаротерапії у комплексному лікуванні хворих сприяло поліпшенню результатів лікування та нормалізації рівня цитокінів при хронізації ранового процесу Ключові слова: гнійні рани кінцівок, озонобаротерапія, бактеріофаг, хірургічнелікування Foundation. One of the mechanisms of ozone effect is its impact on immune state of organism. The work is devoted to study of peculiarities of the state of proinflammatory cytokines at the complex treatment of patients with festering diseases and wounds of limbs. Materials and methods. It was examined and treated 162 patients with festering diseases and wounds of limbs. In the main group 87 patients underwent treatment with ozonebaro-and ozonetherapy, and in the group of comparison 75 patients were treated according to accepted standards. It was studied the content of proinflammatory cytokines TNFα, Il-2, Il-8 in the blood of patients with festering wounds in both groups depending on the volume and area of wounds in the dynamics of treatment. Results. Studying the group of comparison it was established a positive connection between the concentration of TNFα, Il-2, Il-8 and the volume and area of the wound process. It was revealed that hyperactivity of organism that led to the "necrotic" script of development of an alternative phase of the wound process was one of the causes of large necrosis. At chronization of the wound process the level of cytokines decreased on the 15 day comparing with patients with a smooth clinical course that indicated the decrease of reactivity of organism. The offered medical and diagnostic algorithm of the treatment patients using ozone-and ozonebarotherapy favored an improvement of the results of treatment and fastened the healing of wounds. In analysis of the level of cytokines in patients of the main group it was revealed that at the high levels of proinflammatory cytokines the use of the offered methods of treatment favor the decrease of its level and at chronization (with the low level of cytokines) its concentration increases. So the use of ozonebaro-and ozonetherapy leads to normalization of immune response and diminishes the probability of chronization of the wound process. Conclusions. The study of the level of proinflammatory cytokines allows evaluate the clinical course of the wound process objectively, to reveal the tendency to its chronization. The use of ozonebaro and ozonetherapy leads to normalization of immune response at the too high or too low levels of IL-2, IL-8, TNF and diminishes the probability of chronization of the wound process. The data received during the research indicate an efficiency of the offered methods of treatment

Levels of inflammatory mediators in children with pyoinflammatory diseases of soft tissues

“…Over the last decade, there has been an increase in the frequency and severity of purulent-septic diseases in children and the increase in postoperative complications [1,2,5,6]. Thus, this fact makes the problem of treating a surgical infection particularly urgent [1,4,5]. One of the main factors that lead to the emergence and development of various purulent-septic complications, is a violation in the immune system.…”

“…But, inflammation is a protective reaction of the body to damaging factors. In the development of the inflammatory process, a significant role is played by cytokines [1,2,3].…”

“…Inflammation mediators that are most studied in trauma and other infected and aseptic lesions in children and adults include interleukin-1β (IL-1β), interleukin-6 (IL-6), interleukin-8 (IL-8), interleukin and tumor necrosis factor α (TNF-α). According to the literature, these cytokines are mediators of processes aimed at regulating reparation processes in the wound [1,3,5]. And also each of them has a certain role in the development of the wound process.…”