The staining of eosinophil polymorphs and enterochromaffin cells in histological sections

Lendrum, A. C.

doi:10.1002/path.1700560319

Cited by 139 publications

(59 citation statements)

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“…For paraformaldehyde-fixed sections, general histochemical characteristics were assessed following haematoxylin-eosin staining. Mast cells and eosinophils were enumerated following staining with toluidine blue (Enerback, 1966) and carbol-chromotrope (Lendrum, 1944), respectively. Goblet cells in the epithelium and paneth cells in the crypts were detected using Periodic acidSchiffs (PAS) (Mantle and Allen, 1978).…”

Section: Measurements and Sampling Proceduresmentioning

confidence: 99%

The effect of dietary sainfoin (Onobrychis viciifolia) on local cellular responses toTrichostrongylus colubriformisin sheep

et al. 2008

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The effect of sainfoin (Onobrychis viciifolia) hay consumption on the pathophysiology and local cellular responses of growing lambs during infection with Trichostrongylus colubriformis was investigated. Thirty-two lambs, 16 weeks of age, were allocated to 1 of 4 treatment groups (n=8) that were offered either grass (G) or sainfoin (S) hay while concurrently either infected (+), or not (x) with 12 000 L3 T. colubriformis larvae per week for 6 weeks. Liveweight gains were affected by diet (P=0 . 002) and reduced by infection (P<0 . 005). Faecal egg count was reduced in S+ compared to G+from days 35 to 42 (P=0 . 001) ; however, total egg output, worm burdens at day 42 and worm fecundity were similar between diets (P>0 . 05). Feeding sainfoin appeared to enhance immune cell development with tissue eosinophils, mast cells and pan T cells present in greater concentrations in S+ than in G+ animals. However, further studies are required to determine if the enhanced immune cell development is a consequence of a greater nutrient supply or a direct influence of sainfoin metabolites on local inflammatory responses to the gastrointestinal nematode T. colubriformis.

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Section: Measurements and Sampling Proceduresmentioning

confidence: 99%

The effect of dietary sainfoin (Onobrychis viciifolia) on local cellular responses toTrichostrongylus colubriformisin sheep

et al. 2008

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“…Cytospin preparations were prepared in a Shandon Cytospin 2 centrifuge: 100 gAl of BAL fluid was added to each cytospin cup and the samples were centrifuged for 1 min at 1300 r.p.m. Slides were fixed in acetone and stained with haematoxylin and carbol chromotrope according to the method described by Lendrum (1944 …”

Section: Bronchoalveolar Lavagementioning

confidence: 99%

Acute versus chronic administration of phosphodiesterase inhibitors on allergen‐induced pulmonary cell influx in sensitized guinea‐pigs

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1995

British J Pharmacology

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The aims of this study were to determine which phosphodiesterase (PDE) isoenzymes are involved in the control of eosinophil accumulation in the airways of ovalbumin (OVA)‐immunized guinea‐pigs by the use of isoenzyme selective inhibitors and to compare the effects of acute versus chronic administration of PDE isozyme inhibitors on pulmonary cell influx in ovalbumin‐immunized guinea‐pigs. Guinea‐pigs were sensitized and subsequently challenged with aerosolized OVA. Twenty four hours later bronchoalveolar lavage (BAL) was performed to permit assessment of inflammatory cell accumulation. A significant increase in the number of eosinophils was observed in the lavage fluid from OVA‐immunized (13.6 ± 1.4 × 104ml−1 in acute experiments and 10.1 ± 1.4 × 104 ml−1 in chronic experiments) animals compared with sham‐treated controls (5.6 ± 0.6 × 104 ml−1 in acute experiments and 5.1 ± 0.6 × 104 ml−1 in chronic experiments). There was no difference in neutrophil, mononuclear cell or total cell numbers between the two groups. Acute administration of a high dose of selective and non‐selective PDE inhibitors by the i.p. route had no significant effect on eosinophil accumulation in the airways. Chronic administration of a low dose (3 mg kg−1, i.p., twice daily for 7 days) of the type IV PDE inhibitor, RO 20–1724, and the PDE III/IV inhibitor, zardaverine, produced a significant inhibition of eosinophil accumulation (46% and 59% respectively). These results suggest that the type IV PDE isoenzyme plays a role in the control of allergen‐induced eosinophil infiltration into the airways, but indicate that a period of low dose chronic treatment with a type IV or mixed type III/IV PDE inhibitor is necessary for eosinophil accumulation in the airways to be reduced.

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“…Slides were fixed in acetone (BDH, Merck, Poole, Dorset, U.K.) for 10 min and air dried, followed by 10 min in Mayer's hematoxylin (BDH). Slides were washed with tap water and placed in 1% chromotrope 2R, 1% phenol, (11). Microscopic examination showed that 70 -75% of the leukocytes are polymorphonuclear neutrophil granulocytes.…”

Section: Preparation Of Leukocytes From Mouse Peritoneal Cavity For Dmentioning

confidence: 99%

Neurokinin-1 Receptor Agonists Are Involved in Mediating Neutrophil Accumulation in the Inflamed, But Not Normal, Cutaneous Microvasculature: An In Vivo Study Using Neurokinin-1 Receptor Knockout Mice

Cao¹,

Pintér²,

Al-Rashed³

et al. 2000

The Journal of Immunology

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We have used tachykinin neurokinin-1 receptor (NK1 receptor) knockout mice to learn of the link between NK1 receptors and neutrophil accumulation in normal naive skin, as compared with inflamed skin. Intradermal substance P (300 pmol) induced edema formation in wild-type mice, but not in NK1 knockout mice, as expected. However, in contrast to IL-1β (0.3 pmol), substance P did not induce neutrophil accumulation in wild-type mice. IL-1β-induced neutrophil accumulation was similar in wild-type and knockout mice, but a significant (p < 0.05) contributory effect of added NK1 agonists, which by themselves have no effect on neutrophil accumulation in normal skin, was observed. The results support the concept that NK1 agonists such as substance P cannot act on their own to mediate neutrophil accumulation in naive skin and provide direct evidence that in inflamed skin, under certain circumstances, the NK1 receptor can play a pivotal role in modulating neutrophil accumulation during the ongoing inflammatory process. We investigated responses to two inflammatory stimuli (carrageenin and zymosan). Neutrophil accumulation was significantly attenuated (p < 0.001) in carrageenin- but not zymosan-induced inflammation in NK1 knockout mice. The carrageenin (500 μg)-induced response was inhibited (p < 0.05) by a NK1 receptor antagonist, SR140333 (480 nmol/kg i.v. at −5 min), in the wild-type group. The bradykinin B1 and B2 receptor antagonists (desArg9[Leu8]bradykinin and HOE 140) each reduced neutrophil accumulation to carrageenin in wild-type animals (p < 0.05), but did not cause further reduction of the suppressed response of knockout mice. The results provide evidence that kinin receptors participate in NK1 receptor-dependent neutrophil accumulation in inflamed mouse skin.

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The staining of eosinophil polymorphs and enterochromaffin cells in histological sections

Cited by 139 publications

References 1 publication

The effect of dietary sainfoin (Onobrychis viciifolia) on local cellular responses toTrichostrongylus colubriformisin sheep

The effect of dietary sainfoin (Onobrychis viciifolia) on local cellular responses toTrichostrongylus colubriformisin sheep

Acute versus chronic administration of phosphodiesterase inhibitors on allergen‐induced pulmonary cell influx in sensitized guinea‐pigs

Neurokinin-1 Receptor Agonists Are Involved in Mediating Neutrophil Accumulation in the Inflamed, But Not Normal, Cutaneous Microvasculature: An In Vivo Study Using Neurokinin-1 Receptor Knockout Mice

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