2022
DOI: 10.1016/j.ibmb.2022.103738
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The stability and sequence cleavage preference of dsRNA are key factors differentiating RNAi efficiency between migratory locust and Asian corn borer

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Cited by 7 publications
(4 citation statements)
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“…The influence of GC content or thermodynamic stability is a described phenomenon 29 . Other known aspects are the influences of sequence cleavage preference in dsRNA processing by dicer, which was described in Paramecium, moths, or grasshoppers 69 , 70 . As to date, there is no detailed characterization of the RNAi machinery in schistosomes, thus further work is needed to determine the influence of sequence composition of dsRNAs on RNAi efficacy in this parasite.…”
Section: Discussionmentioning
confidence: 99%
“…The influence of GC content or thermodynamic stability is a described phenomenon 29 . Other known aspects are the influences of sequence cleavage preference in dsRNA processing by dicer, which was described in Paramecium, moths, or grasshoppers 69 , 70 . As to date, there is no detailed characterization of the RNAi machinery in schistosomes, thus further work is needed to determine the influence of sequence composition of dsRNAs on RNAi efficacy in this parasite.…”
Section: Discussionmentioning
confidence: 99%
“…Since injection of ds EGFP could induce a strong response of OfDicer2 and OfAgo2 , OfEF1 α and OfCTP8 were selected as two target genes to determine whether the silencing efficiency against the two target genes could be improved by pre-injection of ds EGFP . Previous studies have shown that OfEF1 α can be significantly silenced by tissue culture RNAi in O. furnacalis [ 40 ], and OfCTP8 can be significantly knocked down by injection of ds OfCTP8 in O. furnacalis [ 13 ]. As expected, OfEF1 α could not be significantly silenced in any tissues (hemolymph, integument, and midgut) when O. furnacalis larvae were pre-injected with ddH 2 O.…”
Section: Discussionmentioning
confidence: 99%
“…The dsRNA cleavage pattern also affects RNAi efficacy (Fan et al. , 2022b). However, RNAi as an antiviral defense has been observed in fruit flies (Zhang et al.…”
Section: Systemic Rnai‐based Immunity In Insectsmentioning
confidence: 99%
“…During viral infections, the characteristics of virus-derived small RNA (vsiRNA) is mainly dependent on the activity of Dicer-2, resulting in different vsiRNA and siRNA sizes in different insect species (Santos et al, 2019). The dsRNA cleavage pattern also affects RNAi efficacy (Fan et al, 2022b). However, RNAi as an antiviral defense has been observed in fruit flies (Zhang et al, 2021), aphids (An et al, 2022), and whiteflies (Huang et al, 2021), among others, suggesting the mechanism is pervasive even though the efficacy of RNAi varies among species.…”
Section: Key Factors Contribute To Rnai Efficiency Variation In Agric...mentioning
confidence: 99%