The SPSS Guide to the New Statistical Analysis of Data

Anderson, T. W.; Finn, Jeremy D.; Gerber, Susan B.; Voelkl, Kristin E.

doi:10.1007/978-1-4612-2262-0

Cited by 13 publications

(5 citation statements)

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“…The One Way ANOVA test was used to compare the arithmetic averages of the inhibition regions to the quantities of the different compounds whose biological inhibitory activity was assessed for bacterial species using Duncan's polynomial test at *p < 0.05 (Gerber and Voelkl, 2012).…”

Section: Discussionmentioning

confidence: 99%

Bee chitosan nanoparticles loaded with apitoxin as a novel approach to eradication of common human bacterial, fungal pathogens and treating cancer

Sharaf,

Zahra,

Alharbi

et al. 2024

Front. Microbiol.

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Antimicrobial resistance is one of the largest medical challenges because of the rising frequency of opportunistic human microbial infections across the globe. This study aimed to extract chitosan from the exoskeletons of dead bees and load it with bee venom (commercially available as Apitoxin [Api]). Then, the ionotropic gelation method would be used to form nanoparticles that could be a novel drug-delivery system that might eradicate eight common human pathogens (i.e., two fungal and six bacteria strains). It might also be used to treat the human colon cancer cell line (Caco2 ATCC ATP-37) and human liver cancer cell line (HepG2ATCC HB-8065) cancer cell lines. The x-ray diffraction (XRD), Fourier transform infrared (FTIR), and dynamic light scattering (DLS) properties, ζ-potentials, and surface appearances of the nanoparticles were evaluated by transmission electron microscopy (TEM). FTIR and XRD validated that the Api was successfully encapsulated in the chitosan nanoparticles (ChB NPs). According to the TEM, the ChB NPs and the ChB NPs loaded with Apitoxin (Api@ChB NPs) had a spherical shape and uniform size distribution, with non-aggregation, for an average size of approximately 182 and 274 ± 3.8 nm, respectively, and their Zeta potential values were 37.8 ± 1.2 mV and − 10.9 mV, respectively. The Api@ChB NPs had the greatest inhibitory effect against all tested strains compared with the ChB NPs and Api alone. The minimum inhibitory concentrations (MICs) of the Api, ChB NPs, and Api@ChB NPs were evaluated against the offer mentioned colony forming units (CFU/mL), and their lowest MIC values were 30, 25, and 12.5 μg mL−1, respectively, against Enterococcus faecalis. Identifiable morphological features of apoptosis were observed by 3 T3 Phototox software after Api@ChB NPs had been used to treat the normal Vero ATCC CCL-81, Caco2 ATCC ATP-37, and HepG2 ATCC HB-8065 cancer cell lines for 24 h. The morphological changes were clear in a concentration-dependent manner, and the ability of the cells was 250 to 500 μg mL−1. These results revealed that Api@ChB NPs may be a promising natural nanotreatment for common human pathogens.

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Section: Discussionmentioning

confidence: 99%

Bee chitosan nanoparticles loaded with apitoxin as a novel approach to eradication of common human bacterial, fungal pathogens and treating cancer

Sharaf,

Zahra,

Alharbi

et al. 2024

Front. Microbiol.

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show abstract

“…The results were statistically analyzed by applying the One Way ANOVA test (Analysis of variance), as the arithmetic averages of the inhibition areas were compared according to the concentrations used for the various substances whose biological inhibitory activity we tested for bacterial species using Duncan's polynomial test at a * p < 0.05 (Gerber and Voelkl, 2012).…”

Section: Discussionmentioning

confidence: 99%

Biogenic silver nanoparticles eradicate of Pseudomonas aeruginosa and Methicillin-resistant Staphylococcus aureus (MRSA) isolated from the sputum of COVID-19 patients

et al. 2023

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In recent investigations, secondary bacterial infections were found to be strongly related to mortality in COVID-19 patients. In addition, Pseudomonas aeruginosa and Methicillin-resistant Staphylococcus aureus (MRSA) bacteria played an important role in the series of bacterial infections that accompany infection in COVID-19. The objective of the present study was to investigate the ability of biosynthesized silver nanoparticles from strawberries (Fragaria ananassa L.) leaf extract without a chemical catalyst to inhibit Gram-negative P. aeruginosa and Gram-positive Staph aureus isolated from COVID-19 patient’s sputum. A wide range of measurements was performed on the synthesized AgNPs, including UV–vis, SEM, TEM, EDX, DLS, ζ -potential, XRD, and FTIR. UV-Visible spectral showed the absorbance at the wavelength 398 nm with an increase in the color intensity of the mixture after 8 h passed at the time of preparation confirming the high stability of the FA-AgNPs in the dark at room temperature. SEM and TEM measurements confirmed AgNPs with size ranges of ∼40-∼50 nm, whereas the DLS study confirmed their average hydrodynamic size as ∼53 nm. Furthermore, Ag NPs. EDX analysis showed the presence of the following elements: oxygen (40.46%), and silver (59.54%). Biosynthesized FA-AgNPs (ζ = −17.5 ± 3.1 mV) showed concentration-dependent antimicrobial activity for 48 h in both pathogenic strains. MTT tests showed concentration-dependent and line-specific effects of FA-AgNPs on cancer MCF-7 and normal liver WRL-68 cell cultures. According to the results, synthetic FA-AgNPs obtained through an environmentally friendly biological process are inexpensive and may inhibit the growth of bacteria isolated from COVID-19 patients.

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“…The results were analyzed with a prepared program of SPSS, version 18 (9). Factorial experiment with a completely randomized design (CRD) were used for the reason of using different factors; such as two types of ethanol crude extracts RD, three periods; (24, 48 and 72) Hrs.…”

Section: Discussionmentioning

confidence: 99%

“…The ethanolic solubale part of Andrachna aspera yielded four new trans-2,6-disubstituted-piperidine alkaloid(3). In fact, alkaloids are among the most important active components in natural herbs, and some of these compounds have already been successfully developed into chemotherapeutic drugs, of the plants such as camptothecin (CPT), a famous topoisomerase I (TopI) inhibitor (9,10), and vinblastine, which interacts with tubulin (11).…”

Section: Cell Cycle Analysis By Flow Cytometermentioning

confidence: 99%

Antiproliferative Effects and Apoptosis induction of Ethanolic Extract of Andrachna aspera on Human Rabdomyosarcoma Cells

Hidayat¹

2018

IJCMG

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Arial part of Andrachna aspera ethanolic extract was evaluated by studying their effect on human rabdomyosarcoma (RD) cell line. The cytotoxic activity was determined by anti-proliferative effects when the human rabdomyosarcoma cells were cultured under different concentrations. After 24, 48 and 72h when treated with the ethanolic extract, using cell viability assay. The plant extract showed the strong cytotoxic affect upon the cell like RD with IC50 of 25 µg/ml concentration after 72h, Furthermore, cell cycle analysis showed a significant increase in the accumulation of RD- treated cells at G0/G1 phase (p<0.01), and apoptotic rate also shows significantly increasing in a dose- time dependent manner.The extract of A. aspera induced apoptosis and possessed the most robust anticancer activity, against RD cells

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The SPSS Guide to the New Statistical Analysis of Data

Cited by 13 publications

References 0 publications

Bee chitosan nanoparticles loaded with apitoxin as a novel approach to eradication of common human bacterial, fungal pathogens and treating cancer

Bee chitosan nanoparticles loaded with apitoxin as a novel approach to eradication of common human bacterial, fungal pathogens and treating cancer

Biogenic silver nanoparticles eradicate of Pseudomonas aeruginosa and Methicillin-resistant Staphylococcus aureus (MRSA) isolated from the sputum of COVID-19 patients

Antiproliferative Effects and Apoptosis induction of Ethanolic Extract of Andrachna aspera on Human Rabdomyosarcoma Cells

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