The spread and acquisition of NDM-1: a multifactorial problem

Wailan, Alexander M.; Paterson, David L.

doi:10.1586/14787210.2014.856756

Cited by 81 publications

(67 citation statements)

References 130 publications

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“…The Indian subcontinent is recognized as a major reservoir for bla NDM acquisition and has been suggested as the geographical origin of bla NDM (6,20). In a previous study from our group, 66 NDM-1-producing Gram-negative isolates from stool samples of patients in two Pakistani hospitals were reported (20).…”

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confidence: 82%

“…Since the first report (5), bla NDM has been reported to be harbored by a diverse range of bacterial species, among which the most frequent are within the Acinetobacter genus and Enterobacteriaceae family (6,7). Furthermore, bla NDM has also been identified to reside upon different plasmid replicon types (Inc) among the Enterobacteriaceae family, including IncA/C (8), IncF types (9), IncL/M (10), IncN (11), IncX (12), and IncH (13).…”

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confidence: 99%

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Genetic Contexts of bla _NDM-1 in Patients Carrying Multiple NDM-Producing Strains

Wailan

Sartor

Zowawi

et al. 2015

Antimicrob Agents Chemother

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hThe carbapenem resistance determinant bla NDM-1 has been found in various Gram-negative bacteria and upon different plasmid replicon types (Inc). Here, we present four patients within two hospitals in Pakistan harboring between two and four NDM-1-producing Gram-negative bacilli of different species coresident in their stool samples. We characterize the bla NDM-1 genetic contexts of these 11 NDM-1-producing Gram-negative bacilli in addition to other antimicrobial resistance mechanisms, plasmid replicon profiles, and sequence types (STs) in order to understand the underlying acquisition mechanisms of carbapenem resistance within these bacteria. Two common plasmid types (IncN2 and IncA/C) were identified to carry bla NDM-1 among the six different bacterial species isolated from the four patients. Two of these strains were novel Citrobacter freundii ST 20 and ST 21. The same IncN2-type bla NDM-1 genetic context was found in all four patients and within four different species. The IncA/C-type bla NDM-1 genetic context was found in two different species and in two of the four patients. Combining genetic context characterization with other molecular epidemiology methods, we were able to establish the molecular epidemiological links between genetically unrelated bacterial species by linking their acquisition of an IncN2 or IncA/C plasmid carrying bla NDM-1 for carbapenem resistance. By combining plasmid characterization and in-depth genetic context assessment, this analysis highlights the importance of plasmids in antimicrobial resistance. It also provides a novel approach for investigating the underlying mechanisms of bla NDM-1 -related spread between bacterial species and genera via plasmids.

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confidence: 82%

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confidence: 99%

Genetic Contexts of bla _NDM-1 in Patients Carrying Multiple NDM-Producing Strains

Wailan

Sartor

Zowawi

et al. 2015

Antimicrob Agents Chemother

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“…Genetic variations in the accessory regions of plasmids have contributed to the complexity that underlies the spread of antimicrobial resistance determinants between bacterial hosts. Since its first description (5), bla NDM has been reported on various plasmid Inc types (6), including IncA/C (7,8), IncF types (9), IncL/M (10), IncH (11), IncN types (12)(13)(14), IncX types (15), and IncHI1 types (16), of the family Enterobacteriaceae. However, it may be misleading to assume that all plasmids of the same replicon type are identical, especially among the IncA/C (7,(17)(18)(19) and IncFII (9,20) plasmids.…”

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confidence: 99%

Genomic Characteristics of NDM-Producing Enterobacteriaceae Isolates in Australia and Their bla _NDM Genetic Contexts

Wailan

Paterson

Kennedy

et al. 2016

Antimicrob Agents Chemother

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e bla NDM has been reported in different Enterobacteriaceae species and on numerous plasmid replicon types (Inc). Plasmid replicon typing, in combination with genomic characteristics of the bacterial host (e.g., sequence typing), is used to infer the spread of antimicrobial resistance determinants between genetically unrelated bacterial hosts. The genetic context of bla NDM is heterogeneous. In this study, we genomically characterized 12 NDM-producing Enterobacteriaceae isolated in Australia between 2012 and 2014: Escherichia coli (n ‫؍‬ 6), Klebsiella pneumoniae (n ‫؍‬ 3), Enterobacter cloacae (n ‫؍‬ 2) and Providencia rettgeri (n ‫؍‬ 1). We describe their bla NDM genetic contexts within Tn125, providing insights into the acquisition of bla NDM into Enterobacteriaceae. IncFII-type (n ‫؍‬ 7) and IncX3 (n ‫؍‬ 4) plasmids were the most common plasmid types found. The IncHI1B (n ‫؍‬ 1) plasmid was also identified. Five different bla NDM genetic contexts were identified, indicating four particular plasmids with specific bla NDM genetic contexts (NGCs), three of which were IncFII plasmids (FII-A to -C). Of note, the bla NDM genetic context of P. rettgeri was not conjugative. Epidemiological links between our NDM-producing Enterobacteriaceae were established by their acquisition of these five particular plasmid types. The combination of different molecular and genetic characterization methods allowed us to provide insight into the spread of plasmids transmitting bla NDM .

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“…These isolates have now spread to numerous countries worldwide and have been isolated from both the community and inpatient populations as well as from the environment (1)(2)(3)(4)(5)(6). Twelve NDM enzyme variants have been identified in numerous species of Enterobacteriaceae and common nonfermentative Gram-negative bacilli (7,8).…”

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confidence: 99%

Dissemination of NDM Metallo-β-Lactamase Genes among Clinical Isolates of Enterobacteriaceae Collected during the SMART Global Surveillance Study from 2008 to 2012

Biedenbach¹,

Bouchillon²,

Hackel³

et al. 2015

Antimicrob Agents Chemother

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The spread and acquisition of NDM-1: a multifactorial problem

Cited by 81 publications

References 130 publications

Genetic Contexts of bla _NDM-1 in Patients Carrying Multiple NDM-Producing Strains

Genetic Contexts of bla _NDM-1 in Patients Carrying Multiple NDM-Producing Strains

Genomic Characteristics of NDM-Producing Enterobacteriaceae Isolates in Australia and Their bla _NDM Genetic Contexts

Dissemination of NDM Metallo-β-Lactamase Genes among Clinical Isolates of Enterobacteriaceae Collected during the SMART Global Surveillance Study from 2008 to 2012

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The spread and acquisition of NDM-1: a multifactorial problem

Cited by 81 publications

References 130 publications

Genetic Contexts of bla NDM-1 in Patients Carrying Multiple NDM-Producing Strains

Genetic Contexts of bla NDM-1 in Patients Carrying Multiple NDM-Producing Strains

Genomic Characteristics of NDM-Producing Enterobacteriaceae Isolates in Australia and Their bla NDM Genetic Contexts

Dissemination of NDM Metallo-β-Lactamase Genes among Clinical Isolates of Enterobacteriaceae Collected during the SMART Global Surveillance Study from 2008 to 2012

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Genetic Contexts of bla _NDM-1 in Patients Carrying Multiple NDM-Producing Strains

Genetic Contexts of bla _NDM-1 in Patients Carrying Multiple NDM-Producing Strains

Genomic Characteristics of NDM-Producing Enterobacteriaceae Isolates in Australia and Their bla _NDM Genetic Contexts