The Sporulation Protein SirA Inhibits the Binding of DnaA to the Origin of Replication by Contacting a Patch of Clustered Amino Acids

Rahn-Lee, Lilah; Merrikh, Houra; Grossman, Alan D.; Losick, Richard

doi:10.1128/jb.01390-10

Cited by 42 publications

(79 citation statements)

References 31 publications

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“…Spo0A activates sirA transcription, and SirA blocks further initiation of DNA replication by binding to DnaA (41,51). Inactivation of sirA causes overinitiation of replication (40,51).…”

Section: Discussionmentioning

confidence: 99%

Regulation of Growth of the Mother Cell and Chromosome Replication during Sporulation of Bacillus subtilis

Xenopoulos

Piggot

2011

J Bacteriol

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During spore formation, Bacillus subtilis divides asymmetrically, resulting in two cells with different fates. Immediately after division, the transcription factor F becomes active in the smaller prespore, followed by activation of E in the larger mother cell. We recently showed that a delay in E activation resulted in the novel phenotype of two spores (twins) forming within the same mother cell. Mother cells bearing twins are substantially longer than mother cells with single spores. Here we explore the regulation of the growth and DNA replication of the mother cell. We find that length correlates with chromosome number in the mother cell. We show that replication and growth could occur after asymmetric division in mother cells with no active E . In contrast, when E was active, replication and growth ceased. In growing mother cells, with no active E , Spo0A-directed transcription levels remained low. In the presence of active E , Spo0A-directed gene expression was enhanced in the mother cells. Artificial Spo0A activation blocked mother cell growth in the absence of E . Spo0A activation blocked growth even in the absence of SirA, the Spo0A-directed inhibitor of the initiation of replication. Together, the results indicate that the burst of Spo0A-directed expression along with the activation of E provides mechanisms to block the DNA replication and growth of the mother cell.

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“…Spo0A activates sirA transcription, and SirA blocks further initiation of DNA replication by binding to DnaA (41,51). Inactivation of sirA causes overinitiation of replication (40,51).…”

Section: Discussionmentioning

confidence: 99%

Regulation of Growth of the Mother Cell and Chromosome Replication during Sporulation of Bacillus subtilis

Xenopoulos

Piggot

2011

J Bacteriol

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“…Using this checkpoint, the cell ensures that the 2C DNA content is maintained (322,323,433). The sirA gene was identified in the Spo0A regulon and found to inhibit DNA replication initiation (322,323,433). In addition, it was shown that artificial expression of sirA during vegetative growth caused a block in DNA replication initiation (322,433).…”

Section: Siramentioning

confidence: 99%

Section: Siramentioning

confidence: 99%

“…It was further shown that SirA binds to a group of amino acids (N44, F46, and V47) residing in domain 1 of DnaA, presumably preventing DnaA from establishing an active initiation complex at oriC (323). Mutation of these DnaA residues causes the cell to be unaffected by the inhibitory action of SirA (323).…”

Section: Siramentioning

confidence: 99%

“…As an additional measure to maintain the proper chromosome number during sporulation, B. subtilis can inhibit DNA replication initiation through the action of the checkpoint protein SirA (sporulation inhibitor of replication A) (yneE) (322,323,433). Using this checkpoint, the cell ensures that the 2C DNA content is maintained (322,323,433).…”

Section: Siramentioning

confidence: 99%

See 2 more Smart Citations

DNA Repair and Genome Maintenance in Bacillus subtilis

Lenhart

Schroeder

Walsh

et al. 2012

Microbiol Mol Biol Rev

148

155

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SUMMARY From microbes to multicellular eukaryotic organisms, all cells contain pathways responsible for genome maintenance. DNA replication allows for the faithful duplication of the genome, whereas DNA repair pathways preserve DNA integrity in response to damage originating from endogenous and exogenous sources. The basic pathways important for DNA replication and repair are often conserved throughout biology. In bacteria, high-fidelity repair is balanced with low-fidelity repair and mutagenesis. Such a balance is important for maintaining viability while providing an opportunity for the advantageous selection of mutations when faced with a changing environment. Over the last decade, studies of DNA repair pathways in bacteria have demonstrated considerable differences between Gram-positive and Gram-negative organisms. Here we review and discuss the DNA repair, genome maintenance, and DNA damage checkpoint pathways of the Gram-positive bacterium Bacillus subtilis. We present their molecular mechanisms and compare the functions and regulation of several pathways with known information on other organisms. We also discuss DNA repair during different growth phases and the developmental program of sporulation. In summary, we present a review of the function, regulation, and molecular mechanisms of DNA repair and mutagenesis in Gram-positive bacteria, with a strong emphasis on B. subtilis.

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A new role for monomeric ParA/Soj in chromosome dynamics in Bacillus subtilis

Roberts

2023

MicrobiologyOpen

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ParABS (Soj‐Spo0J) systems were initially implicated in plasmid and chromosome segregation in bacteria. However, it is now increasingly understood that they play multiple roles in cell cycle events in Bacillus subtilis , and possibly other bacteria. In a recent study, monomeric forms of ParA/Soj have been implicated in regulating aspects of chromosome dynamics during B. subtilis sporulation. In this commentary, I will discuss the known roles of ParABS systems, explore why sporulation is a valuable model for studying these proteins, and the new insights into the role of monomeric ParA/Soj. Finally, I will touch upon some of the future work that remains.

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The Sporulation Protein SirA Inhibits the Binding of DnaA to the Origin of Replication by Contacting a Patch of Clustered Amino Acids

Cited by 42 publications

References 31 publications

Regulation of Growth of the Mother Cell and Chromosome Replication during Sporulation of Bacillus subtilis

Regulation of Growth of the Mother Cell and Chromosome Replication during Sporulation of Bacillus subtilis

DNA Repair and Genome Maintenance in Bacillus subtilis

A new role for monomeric ParA/Soj in chromosome dynamics in Bacillus subtilis

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