The Sponge Implant Model of Angiogenesis

Andrade, Sílvia Passos; Ferreira, Mônica Alves Neves Diniz

doi:10.1007/978-1-4939-3628-1_23

Cited by 7 publications

(6 citation statements)

References 21 publications

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“…A common attempt to repair or replace biological tissues is the implantation of biomedical devices or synthetic matrices. Often, an adverse foreign body reaction occurs in the vicinity of implanted devices [152], associated with inflammatory reactions which can induce vessel growth around the implants [153]. This is a result from persistent inflammatory stimuli, which mediate series of cellular alterations by various surrounding cells.…”

Section: Endothelial Cells and Angiogenesismentioning

confidence: 99%

Vascular Endothelial Cell Biology: An Update

Krüger‐Genge

Blocki

Franke

et al. 2019

IJMS

703

495

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The vascular endothelium, a monolayer of endothelial cells (EC), constitutes the inner cellular lining of arteries, veins and capillaries and therefore is in direct contact with the components and cells of blood. The endothelium is not only a mere barrier between blood and tissues but also an endocrine organ. It actively controls the degree of vascular relaxation and constriction, and the extravasation of solutes, fluid, macromolecules and hormones, as well as that of platelets and blood cells. Through control of vascular tone, EC regulate the regional blood flow. They also direct inflammatory cells to foreign materials, areas in need of repair or defense against infections. In addition, EC are important in controlling blood fluidity, platelet adhesion and aggregation, leukocyte activation, adhesion, and transmigration. They also tightly keep the balance between coagulation and fibrinolysis and play a major role in the regulation of immune responses, inflammation and angiogenesis. To fulfill these different tasks, EC are heterogeneous and perform distinctly in the various organs and along the vascular tree. Important morphological, physiological and phenotypic differences between EC in the different parts of the arterial tree as well as between arteries and veins optimally support their specified functions in these vascular areas. This review updates the current knowledge about the morphology and function of endothelial cells, particularly their differences in different localizations around the body paying attention specifically to their different responses to physical, biochemical and environmental stimuli considering the different origins of the EC.

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Section: Endothelial Cells and Angiogenesismentioning

confidence: 99%

Vascular Endothelial Cell Biology: An Update

Krüger‐Genge

Blocki

Franke

et al. 2019

IJMS

703

495

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“…We hypothesize that this sesquiterpene might modulate the components of the fibrovascular tissue induced by a synthetic matrix of polyether-polyurethane in mice. In this model, inflammatory, angiogenic, and fibrogenic markers can be identified and quantified including cell influx, blood vessel formation, cytokine production, and extracellular matrix deposition. , We found that inflammation and fibrogenesis, but not angiogenesis, in subcutaneous implants were down-regulated by polygodial.…”

mentioning

confidence: 76%

“…In this model, inflammatory, angiogenic, and fibrogenic markers can be identified and quantified including cell influx, blood vessel formation, cytokine production, and extracellular matrix deposition. 18,19 We found that inflammation and fibrogenesis, but not angiogenesis, in subcutaneous implants were down-regulated by polygodial.…”

mentioning

confidence: 85%

“…Polygodial. 1 H NMR (CDCl 3 , 500 MHz) δ 9.50 (d, J = 4.4 Hz, H-11), 9.43 (s, H-12), 7.12 (ddd, J = 7.5, 2.2, and 2.1 Hz, H-7), 2.78 (m, H-9), 2.49 (m, H-6α), 2.30 (m, H-6β), 1.83 (m, H-1β), 1.52−1.47 (m, H-2), 1.45 (m, H-3β), 1.36 (m, H-1α), 1.23 (m, H-5), 1.20 (m, H-3α), 0.93 (s, H-14), 0.92 (s, H-15), 0.90 (s, H-13); 13 (12), 163 (18), 145 (16), 135 (22), 121 (100), 110 (81), 91 (64), 79 (47), 67 (48) Sponge Disc Implantation and Polygodial Treatment. Polyether-polyurethane sponge discs (Vitafoam Ltd., Manchester, UK), 5 mm thick × 8 mm diameter, were used as the implanted material to create the inflammatory environment.…”

Section: ■ Experimental Sectionmentioning

confidence: 99%

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Sesquiterpene Polygodial from Drimys brasiliensis (Winteraceae) Down-Regulates Implant-Induced Inflammation and Fibrogenesis in Mice

et al. 2020

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Drimys brasiliensis (Winteraceae) has been investigated in traditional medicine for its anti-inflammatory properties to treat gastric ulcers and allergic and respiratory system diseases as well as for cancer treatment. In this work, we investigate the ability of the sesquiterpene polygodial, isolated from D. brasiliensis stem barks, to modulate the chronic inflammatory response induced by polyester-polyurethane sponge implants in C57BL/6J mice. Daily treatment with polygodial inhibited the macrophage content in the implants as determined by the activity of the N-acetyl-β-d-glucosaminidase enzyme as well as decreased the levels of CXCL1/KC and CCL2/JE/MCP-1 pro-inflammatory chemokines and the presence of mast cells along the formed fibrovascular tissue. Similarly, the deposition of a new extracellular matrix (total collagen and type I and III collagen fibers) as well as the production of the TGF-β1 cytokine were attenuated in implants treated with polygodial, showing for the first time its antifibrogenic capacity. The hemoglobin content, the number of newly formed vessels, and the levels of VEGF cytokine, which were used as parameters for the assessment of the neovascularization of the implants, did not change after treatment with polygodial. The anti-inflammatory and antifibrogenic effects of polygodial over the components of the granulation tissue induced by the sponge implant indicate a therapeutic potential for the treatment of inflammatory diseases associated with the development of fibrovascular tissue.

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“…Using a well-characterized mouse model of peritonitis, they compared the immuno-pathological responses (e.g. inflammatory cell infiltration, angiogenesis and fibrosis) in three inbred strains (DBA-1, BALB/c, C57BL/6) and one outbred strain (Swiss) [ 47 , 48 ]. These investigators observed highly variable responses among the four different groups of mice at 7 days following implantation.…”

Section: Introductionmentioning

confidence: 99%

Genomic, microbial and environmental standardization in animal experimentation limiting immunological discovery

et al. 2020

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Background: The use of inbred mice housed under standardized environmental conditions has been critical in identifying immuno-pathological mechanisms in different infectious and inflammatory diseases as well as revealing new therapeutic targets for clinical trials. Unfortunately, only a small percentage of preclinical intervention studies using well-defined mouse models of disease have progressed to clinically-effective treatments in patients. The reasons for this lack of bench-to-bedside transition are not completely understood; however, emerging data suggest that genetic diversity and housing environment may greatly influence muring immunity and inflammation. Results: Accumulating evidence suggests that certain immune responses and/or disease phenotypes observed in inbred mice may be quite different than those observed in their outbred counterparts. These differences have been thought to contribute to differing immune responses to foreign and/or auto-antigens in mice vs. humans. There is also a growing literature demonstrating that mice housed under specific pathogen free conditions possess an immature immune system that remarkably affects their ability to respond to pathogens and/or inflammation when compared with mice exposed to a more diverse spectrum of microorganisms. Furthermore, recent studies demonstrate that mice develop chronic cold stress when housed at standard animal care facility temperatures (i.e. 22-24°C). These temperatures have been shown alter immune responses to foreign and auto-antigens when compared with mice housed at their thermo-neutral body temperature of 30-32°C. Conclusions: Exposure of genetically diverse mice to a spectrum of environmentally-relevant microorganisms at housing temperatures that approximate their thermo-neutral zone may improve the chances of identifying new and more potent therapeutics to treat infectious and inflammatory diseases.

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The Sponge Implant Model of Angiogenesis

Cited by 7 publications

References 21 publications

Vascular Endothelial Cell Biology: An Update

Vascular Endothelial Cell Biology: An Update

Sesquiterpene Polygodial from Drimys brasiliensis (Winteraceae) Down-Regulates Implant-Induced Inflammation and Fibrogenesis in Mice

Genomic, microbial and environmental standardization in animal experimentation limiting immunological discovery

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