The spirotetramat inhibits growth and reproduction of silkworm by interfering with the fatty acid metabolism

He, Ping; Wei, Erjun; Wang, Runpeng; Wang, Qiang; Zhang, Shujun; Tang, Xudong; Zhu, Feng; Shen, Zhongyuan

doi:10.1016/j.pestbp.2022.105282

Cited by 8 publications

(1 citation statement)

References 39 publications

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“…Interestingly, under chlorantranilamide treatment, these pathway genes were also altered which affected the development time of C. suppressalis larvae [ 22 , 23 ]. Lipids play an important role in insect reproduction [ 24 , 25 , 26 ], and studies have demonstrated that changes in lipid content affect silkworm reproduction [ 27 ]. In our study, 31 DEGs were associated with glycerolipid metabolism, sphingolipid metabolism, steroid biosynthesis, fatty acid elongation, glycerophosholipid metabolism, fatty acid degradation, fatty acid biosynthesis, fatty acid metabolism and unsaturated fatty acid biosynthesis.…”

Section: Discussionmentioning

confidence: 99%

Transcriptomic Analysis Reveals the Detoxification Mechanism of Chilo suppressalis in Response to the Novel Pesticide Cyproflanilide

Zhou

Qiu

et al. 2023

IJMS

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Chilo suppressalis is one of the most damaging rice pests in China’s rice-growing regions. Chemical pesticides are the primary method for pest control; the excessive use of insecticides has resulted in pesticide resistance. C. suppressalis is highly susceptible to cyproflanilide, a novel pesticide with high efficacy. However, the acute toxicity and detoxification mechanisms remain unclear. We carried out a bioassay experiment with C. suppressalis larvae and found that the LD10, LD30 and LD50 of cyproflanilide for 3rd instar larvae was 1.7 ng/per larvae, 6.62 ng/per larvae and 16.92 ng/per larvae, respectively. Moreover, our field trial results showed that cyproflanilide had a 91.24% control efficiency against C. suppressalis. We investigated the effect of cyproflanilide (LD30) treatment on the transcriptome profiles of C. suppressalis larvae and found that 483 genes were up-regulated and 305 genes were down-regulated in response to cyproflanilide exposure, with significantly higher CYP4G90 and CYP4AU10 expression in the treatment group. The RNA interference knockdown of CYP4G90 and CYP4AU10 increased mortality by 20% and 18%, respectively, compared to the control. Our results indicate that cyproflanilide has effective insecticidal toxicological activity, and that the CYP4G90 and CYP4AU10 genes are involved in detoxification metabolism. These findings provide an insight into the toxicological basis of cyproflanilide and the means to develop efficient resistance management tools for C. suppressalis.

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