The Spermicide Effect from Methanol Extract of Kalangkala Seed (Litsea angulata) to Spermatozoa Mice (Mus musculus)

Akmal, Rommy; Kuswanto, Kuswanto; Fahrunnisa, Syarifah; Rahmi, Ridha Aulia; Bayanil, Nur Ema Putri; Nurliani, Anni

doi:10.19184/ams.v2i2.2776

Cited by 2 publications

(2 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Saponins and tannins are cytotoxic or spermicidal on spermatozoa which affect cell membrane permeability and disrupted nutrient as a source of sperm energy so that spermatozoa will lack energy and decrease sperm motility. 21,22 Toxicity of saponins also inhibits certain enzymes such as hyaluronidase and acrosine. 22 However, because of the high antioxidant content such as terpenoids, flavonoids, and alkaloids, it is possible to suppress some of spermicide effect by incubating chloroform and methanol fractions.…”

Section: Discussionmentioning

confidence: 99%

Photochemical and bioactivity examination of fractionated saluang belum root extract (Lavanga sarmentosa) on in-vitro human sperm motility

Permatasari,

Syarpin,

Pujianto

2023

JKKI

View full text Add to dashboard Cite

Background: Saluang belum (Lavanga sarmentosa) is one of Kalimantan's typical plants, which is as efficacious as a traditional medicine to increase sexual activity and male fertility. Based on previous studies, the content of flavonoid and steroid in 70% ethanol extract of L. sarmentosa were able to affect sperm quality of mice. Studies related to L. sarmentosa are still limited for phytochemical test and their bioactivity on human spermatozoa motility in vitro.Objective: This study is to perform phytochemical tests of compound content in fractionation with eluents of high and low polarity, namely methanol and chloroform, and then to test their bioactivity on the motility of human spermatozoa in vitro.Methods: L. sarmentosa was extracted with 96% ethanol and fractionated by using a vacuum chromatography column with chloroform and methanol as the eluent. Then obtained samples were analysed by a quantitative phytochemical test. The samples used in-vitro human spermatozoa were divided into eleven groups: control group, group administered with L. sarmentosa extract eluent chloroform of 10, 50, 100, 500, and 1000 ng/mL, and same concentration with extract eluent methanol. Furthermore, the sperm motility was analysed by using a Computer Assisted Sperm Analyser (CASA). Results: The methanol and chloroform fraction of L. sarmentosa root extract contained metabolites, namely terpenoids, flavonoids, steroids, alkaloids, saponins, and tannins. The sperm motility increased significantly at the treatment group of the methanol and chloroform fractions compared to the control group. There was a significant difference between the sperm motility incubated with methanol and that with chloroform fraction at concentration 500 and 1000 ng/mL.Conclusion: The results of sperm motility were higher in the methanol fraction than those in the chloroform fraction.

show abstract

Section: Discussionmentioning

confidence: 99%

Photochemical and bioactivity examination of fractionated saluang belum root extract (Lavanga sarmentosa) on in-vitro human sperm motility

Permatasari,

Syarpin,

Pujianto

2023

JKKI

View full text Add to dashboard Cite

show abstract

“…Susi (2018) menyatakan bahwa komponen asam amino yang dominan pada buah malek adalah asam glutamate (109,7μg/g), dan komponen asam lemak yang paling dominan adalah asam dokosadienoat (221,4μg/mg) sedangkan bji mengandung asam arachidonat (190,7μg/mg). Uji Kualitatif senyawa fitokimia menunjukkan bahwa pada buah malek mengandung alkaloid sedangkan pada biji mengandung alkaloid, flavonoid dan kuinonyang mempunyai aktivitas anti fertilitas, sedangkan kulit kayu, cabang dan daun mempunyai aktivitas sebagai anti bakteri (Akmal et al, 2016;Kuspradini et al, 2019).…”

Section: Pendahuluanunclassified

Aktivitas Anti Inflamasi Ekstrak Biji Litsea Garciae Vidal Pada Odema Telapak Kaki Dan Gambaran Histologis Kaki Mencit

Sari

Sulistiany

2021

BERITABIOLOGI

View full text Add to dashboard Cite

Litsea garciae or malek is a native species from Borneo and belongs to the Lauraceae family. Scientifically, the use of these plants is not widely known. The purpose of this study was to determine the anti-inflammatory activity of Litsea garciae seeds against edema in mice feet and to determine the histology of the carrageenan-induced integument thickness of mice. The study used a completely randomized design with 5 treatment groups. The treatment group consisted of a negative treatment group (K1), a positive treatment group (K2) with 3 different dosages of Malek seed extract groups. The treatment doses used were Malek seed extract at a dose of 0.625 mg/g BW (P1), 1.25 mg/g BW (2), and 2.50 mg/g BW (P3). Edema percentage and integument thickness were analyzed by the One-Way ANOVA test with (α = 0.05). The results showed that the Malek seed extract dose of 0.625 mg/g BW had anti-inflammatory activity against edema of the mice's feet. The percentage of inflammation with doses of 0.625 mg/g BW, 1.25 mg/g BW and 2.5 mg/g BW was 31.10%, 22.58%, and 25.83%. The percentage of reduction in inflammation in the positive control treatment of Na-diclofenac (42.70%) was significantly differ-ent from the treatment of Malek seed extract at a dose of 0.0625–2.5 mg/g BW. The percentage of reduction in inflammation in the group treated with 1.25 mg/g BW (P2) Malek seed extract was not significantly different from the 2.5 mg/g BW (P3) treatment of Malek seed extract. The results of histological observations of mice's feet showed that the extract of Malek seeds did not have a significant effect on the thickness of the carrageenan-induced mice leg integument.

show abstract

The Spermicide Effect from Methanol Extract of Kalangkala Seed (Litsea angulata) to Spermatozoa Mice (Mus musculus)

Cited by 2 publications

References 0 publications

Photochemical and bioactivity examination of fractionated saluang belum root extract (Lavanga sarmentosa) on in-vitro human sperm motility

Photochemical and bioactivity examination of fractionated saluang belum root extract (Lavanga sarmentosa) on in-vitro human sperm motility

Aktivitas Anti Inflamasi Ekstrak Biji Litsea Garciae Vidal Pada Odema Telapak Kaki Dan Gambaran Histologis Kaki Mencit

Contact Info

Product

Resources

About