The sperm chromatin structure assay (SCSA) as prognostic factor in IVF/ICSI program

Miciñski, Piotr; Pawlicki, Krzysztof; Wielgus, Ewa; Bochenek, M.; Tworkowska, Iwona

doi:10.1016/s1642-431x(12)60095-3

Cited by 44 publications

(30 citation statements)

References 10 publications

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“…As with IVF studies, these ICSI studies are quite heterogeneous in terms of design, inclusion/exclusion criteria, and the sperm DNA damage test employed. In keeping with a recent analysis [Collins et al 2008], the results of this updated meta-analysis on ICSI studies indicate that sperm DNA damage is not related to ICSI pregnancy rates (combined OR of 1.15, 95% 0.90, 1.55, p = 0.65) [Benchaib et al 2007;Boe-Hansen et al 2006;Borini et al 2006;Bungum et al 2007;Check et al 2005;Gandini et al 2004;Hammadeh et al 1996;Henkel et al 2003;Host et al 2000;Huang et al 2005;Lin et al 2008;Micinski et al 2009;Tarozzi et al 2009;Zini et al 2005]. The characteristics of the 14 ICSI studies that could be evaluated are shown in Tables 4 and 5.…”

Section: In Vitro Fertilization With Intracytoplasmic Sperm Injectionsupporting

confidence: 62%

“…Abbreviations: %hDD = proportion of samples with high sperm DNA damage; Sens = sensitivity; Spec = specificity; PPV = positive predictive value; NPV = negative predictive value; OR = odds ratio; ABlue = aniline blue; TUNEL = terminal deoxynucleotidyl transferase-mediated dUTP nick endlabeling; SCSA = sperm chromatin structure assay; CMA3 = chromomycin A3. Bungum et al 2007 Male factor CP Prospective <40 years, FSH<12 Evenson et al 1999Lin et al 2008 Male factor CP Prospective <40 years, FSH<15 Evenson et al 1999Micinski et al 2009 Unselected CP Prospective <38 years Filatov et al 1999Tarozzi et al 2009 Male factor CP Unspecified Unspecified ROC analysis…”

Section: In Vitro Fertilization With Intracytoplasmic Sperm Injectionmentioning

confidence: 99%

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Are sperm chromatin and DNA defects relevant in the clinic?

Zini

2011

Systems Biology in Reproductive Medicine

227

196

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There has been an increase in the use of sperm DNA and chromatin integrity tests in the evaluation of the infertile man with the hypothesis that these tests may better diagnose infertility and predict reproductive outcomes. This review discusses the etiology of sperm DNA damage, briefly describing the tests of sperm DNA damage, and evaluates the relationship between sperm DNA damage and reproductive outcomes. A systematic review of the literature allows us to conclude that sperm DNA damage is associated with lower natural, intra-uterine insemination (IUI), and in vitro fertilization (IVF) pregnancy rates. Studies to date have not shown a clear association between sperm DNA and chromatin defects and pregnancy outcomes after intra-cytoplasmic sperm injection (ICSI). However, we cannot exclude the possibility that very high levels of DNA damage will impact on ICSI outcomes. In couples undergoing IVF or ICSI, there is evidence to show that sperm DNA damage is associated with an increased risk of pregnancy loss. A limitation of this systematic review and meta-analysis is that it does not address the heterogeneity of the individual study characteristics. Although the clinical utility of tests of sperm DNA damage remains to be firmly established, the data suggest that there is clinical value in testing couples prior to assisted reproductive technologies (ARTs IUI, IVF, and ICSI) and in those couples with recurrent miscarriages. Additional, well-designed prospective studies are needed before testing becomes a routine part of patient care.

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Section: In Vitro Fertilization With Intracytoplasmic Sperm Injectionsupporting

confidence: 62%

Section: In Vitro Fertilization With Intracytoplasmic Sperm Injectionmentioning

confidence: 99%

Are sperm chromatin and DNA defects relevant in the clinic?

Zini

2011

Systems Biology in Reproductive Medicine

227

196

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“…Twenty studies [7][8][9][10][11][12][13][14][15][16][17][18][19][20][21][22][23][24][25][26] were indentified for our meta-analysis. The flow chart of the process for the identification of the studies were shown in Fig.…”

Section: Resultsmentioning

confidence: 99%

“…A total of 15 studies with CP as the outcome measurement was included in the meta-analysis [7,8,10,12,[15][16][17][19][20][21][22][23][24][25][26]. These studies were divided into 3 groups according to the DFI cutoff value (>27 %, 15-27 %, ≤15 %) as described previously.…”

Section: Dfi and Cpmentioning

confidence: 99%

“…As for the articles included if the DFI threshold value was less than 15 % [7,12,15,16,[20][21][22][23], the pooled OR of all studies (OR (95%CI) = 0.886 (0.719-1.090), p=0.252) and IVF subgroup (OR (95%CI) = 0.759 (0.571-1.008), p=0.057) [7,12,20,15,21] and ICSI (OR (95%CI) = 1.062 (0.780-1.446), p=0.703) subgroup [7,12,15,16,22,23] showed that DFI was not associated with CP. We found no heterogeneity existed with I squared = 0 % in the overall and IVF and ICSI subgroup (see Fig.…”

Section: Dfi and Cpmentioning

confidence: 99%

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Sperm DNA fragmentation index and pregnancy outcome after IVF or ICSI: a meta-analysis

Zheng

Zhu

Jiang

et al. 2014

J Assist Reprod Genet

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Purpose The purpose of this study was to carry out a metaanalysis for a comprehensive understanding and estimation of the association between sperm DNA Fragmentation Index (DFI) and pregnancy outcome after in vitro fertilization (IVF) or intracytoplasmic sperm injection (ICSI) treatment. Methods Studies concerning the link of DFI with pregnancy outcome were included after literature search of database PUBMED, EMBASE, MEDLINE. Related information was extracted from the eligible studies by two independent authors and a meta-analysis was conducted by using STATA 12.0 software. Pregnancy outcomes consisted of biochemical pregnancy (BP), clinical pregnancy (CP) and pregnancy loss (PL). The studies included for meta-analysis were divided into three groups according to the DFI threshold value (DFI >27 %, 15-27 %, ≤15 % group). The odds ratio (OR ) and their 95 % confidence intervals (95 % CIs) were used to evaluate the association between DFI and pregnancy outcome. Results Twenty articles were included in our meta-analysis. The results indicated that infertile couples were more likely to get pregnant if DFI was less than threshold value (For threshold value > 27 % and 15-27 % group, combined overall OR (95 % CI) = 1.437 (1.186-1.742), 1.639 (1.093-2.459) respectively). However, when stratified by DFI detection methods, using sperm chromatin structure assay (SCSA) as the DFI test method, the results indicated a similar CP rate between groups with a high DFI or a lower DFI value (SCSA, For threshold value >27 % and 15-27 % group, combined overall OR (95 % CI) = 1.242(0.978-1.577), 1.480(0.921-2.377) respectively). The meta-analysis based on BP (overall OR (95 % CI) = 0.952 (0.697-1.302)) and PL((For DFI >27 %, 15-27 %, ≤15 % group, OR (95 % CI) = 0.786 (0.491-1.258), 1.509 (0.655-3.476), (0.538 (0.264-1.097) respectively) outcome yielded nonsignificant results. Conclusions The predication value of DFI for IVF or ICSI outcome is not confirmed in our meta-analysis. Further better designed studies with larger subjects involved are needed to better address this issue.

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Clinical Utility of Sperm DNA Integrity Tests

Zini

2011

Sperm Chromatin

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The sperm chromatin structure assay (SCSA) as prognostic factor in IVF/ICSI program

Cited by 44 publications

References 10 publications

Are sperm chromatin and DNA defects relevant in the clinic?

Are sperm chromatin and DNA defects relevant in the clinic?

Sperm DNA fragmentation index and pregnancy outcome after IVF or ICSI: a meta-analysis

Clinical Utility of Sperm DNA Integrity Tests

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