The Spectral Determination of Chlorophylls a and b, as well as Total Carotenoids, Using Various Solvents with Spectrophotometers of Different Resolution

Wellburn, A. R.

doi:10.1016/s0176-1617(11)81192-2

Cited by 4,499 publications

(2,490 citation statements)

References 15 publications

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“…After correcting sample absorbance at 440 with that of the blank at 440 nm and the sample at 750 nm, Pt unit was calculated with the formula of Cuthbert and del Giorgio (1992). Chlorophyll a concentration was determined spectrophotometrically after hot methanol extraction using the absorption coefficients determined by Wellburn (1994).…”

Section: Laboratory Measurementsmentioning

confidence: 99%

Unique picoeukaryotic algal community under multiple environmental stress conditions in a shallow, alkaline pan

et al. 2013

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Winter phytoplankton communities in the shallow alkaline pans of Hungary are frequently dominated by picoeukaryotes, sometimes in particularly high abundance. In winter 2012 the ice-covered alkaline Zab-szék pan was found to be extraordinarily rich in picoeukaryotic green algae (42-82 × 10 6 cells ml -1 ) despite the simultaneous presence of multiple stressors (low temperature and light intensity with high pH and salinity). The maximum photosynthetic rate of the picoeukaryote community was 1.4 µg C µg chlorophyll a -1 h -1 at 125 µmol m -2 s -1 .The assimilation rates compared with the available light intensity measured on the field show that the community was considerably light-limited. Estimated areal primary production was 2 180 mg C m -2 d -1 . On the basis of the 18S rRNA gene analysis (cloning and DGGE), the community was phylogenetically heterogeneous with several previously undescribed chlorophyte lineages, which indicates the ability of picoeukaryotic communities to maintain high genetic diversity under extreme conditions.

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Section: Laboratory Measurementsmentioning

confidence: 99%

Unique picoeukaryotic algal community under multiple environmental stress conditions in a shallow, alkaline pan

et al. 2013

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“…The fresh leaves were ground in 80% acetone as quickly as possible at room temperature, and the chlorophyll (Chl) contents (Chla, Chlb and Chla/b) were determined using a spectrophotometer (SCHOTT UVLINE 9400, German) at 470, 646 and 663 nm and by the calculations described by Wellburn (1994).…”

Section: Chlorophyll Contentmentioning

confidence: 99%

Growth and physiological responses to water and nutrient stress in oil palm

Sun¹,

Cao²,

Shao³

et al. 2011

Afr. J. Biotechnol.

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The research was conducted to detect changes in growth, physiology and nutrient concentration in response to two watering regimes (well-watered and water-stress conditions) and to two nutrient regimes (with or without fertilization) of oil palm. Under stress conditions, changes in plant growth, dry matter allocation, relative water content, leaf relative conductivity, leaf N, P and K concentration are usually observed. These characteristics and related parameters were determined and the experiment results are listed as follows: (1) fertilization promoted the growth of oil palm under well-watered conditions, while under water stress conditions its effects on growth was negative. The ratio of root/shoot was increased under water stress condition; (2) relative water content and chlorophyll a/b content were gradually decreased while leaf relative conductivity was increased quickly under water and nutrient stress conditions during the experiment. It is obvious that water stress had a greater influence than nutrient stress on these parameters; (3) water and nutrient stress decreased leaf nitrogen and phosphorus concentration but increased potassium concentration; the combination of water and nutrient stress made significant effects on nitrogen and phosphorus concentration, but no significant effects on potassium concentration. Moreover, deficiency of both water and nutrients in combination had the greatest impact on changes in these traits of oil palm.

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“…Freshly collected water samples were transferred to the laboratory within two hours in dark conditions (thermo boxes) for further analyses. Chlorophyll a concentration was determined spectrophotometrically (Shimadzu 160A UV-VIS spectrophotometer) after hot methanol extraction using the absorption coefficients determined by Wellburn (1994).…”

mentioning

confidence: 99%

Picophytoplankton predominance in hypersaline lakes (Transylvanian Basin, Romania)

et al. 2014

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The occurrence and importance of photoautotrophic picoplankton (PPP, cells with a diameter <2 μm) was studied along a trophic and salinity gradient in hypersaline lakes of the Transylvanian Basin (Romania). The studied lakes were found to be rich in PPP, with abundances (maximum: 7.6 x 10 6 cells mL -1 ) higher than in freshwater and marine environments of similar trophic conditions. The contribution of PPP to the total phytoplankton biovolume did not decrease with increasing trophic state as it was generally found in other aquatic environments. Regardless of the trophic conditions, the contribution of PPP could reach 90-100% in these hypersaline lakes.We hypothesized that the PPP predominance might be the result of the low grazing pressure, since heterotrophic nanoflagellates (the main grazers of PPP) were absent in the studied samples.There were significant differences in community composition among the lakes along the salinity gradient. CyPPP predominated in less saline waters (mainly below 5%), while EuPPP were present along the entire salinity range (up to 18.7%), dominating the phytoplankton between 3 and 13% salinity. Above 13% salinity, the phytoplankton was composed mainly of Dunaliella species.

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The Spectral Determination of Chlorophylls a and b, as well as Total Carotenoids, Using Various Solvents with Spectrophotometers of Different Resolution

Cited by 4,499 publications

References 15 publications

Unique picoeukaryotic algal community under multiple environmental stress conditions in a shallow, alkaline pan

Unique picoeukaryotic algal community under multiple environmental stress conditions in a shallow, alkaline pan

Growth and physiological responses to water and nutrient stress in oil palm

Picophytoplankton predominance in hypersaline lakes (Transylvanian Basin, Romania)

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