The specificity of the methyl green-pyronin stain for nucleic acids

Taft, Edgar B.

doi:10.1016/0014-4827(51)90021-3

Cited by 60 publications

(11 citation statements)

References 14 publications

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“…Some might be considered still Gram-positive but others were so faintly and irregularly tinged as to appear like eroded remnants of the original solid droplets. Similar incomplete removal of stainable material by ribonuclease in other experiments (7) has been interpreted as an indication of the presence of a protein residue; this conclusion would seem applicable in the case of the droplets. The fact remains that something was removed by the enzyme, as indicated by the altered staining reaction of the droplets.…”

Section: The Significance O/the Gram Positivity Of Th Dropletssupporting

confidence: 71%

Cellular Mechanisms of Protein Metabolism in the Nephron

Oliver

Moses

MacDowell

et al. 1954

Journal of Experimental Medicine

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We have shown (1) that the droplets forming in the epithelial cells of the proximal convolution of the rat kidney after the administration of various proteins give a strongly positive supravital reaction with dilute Janus green. This observation indicates that they are not simple accumulations of the absorbed material and suggests that they are complex objects composed of a mixture of the absorbed protein and mitochondrial elements, the latter derived from the rodlets of the renal cells which were observed to disappear concomitantly with the formation of the droplets. From such a hypothesis it would follow that the enzymatic content of the mitochondria is thus brought into immediate contact with the substrate, absorbed protein, which is ultimately to be digested inside the cell. The prompt reduction of the dye within the droplet supported this assumption.The basis of these inferences is a staining reaction with a dye and its subsequent reduction in the droplet, and since the chemical or physical mechanisms involved in the reaction are unknown, more information is needed. It should, for example, be possible to demonstrate in the droplet those chemical substances which occur in the mitochondrial rodlets; the present study is an attempt to do so by means of the methods of histochemistry. The rigorous validity of many of these methods is open to question; some of them might better be termed more or less specific "staining reactions;" but these and other shortcomings have been thoroughly considered by others and are generally recognized. Our

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Section: The Significance O/the Gram Positivity Of Th Dropletssupporting

confidence: 71%

Cellular Mechanisms of Protein Metabolism in the Nephron

Oliver

Moses

MacDowell

et al. 1954

Journal of Experimental Medicine

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“…The nuclear pellet was resuspended in NRB and stored at À80°C. An aliquot of the nuclear suspension was diluted with buffer C and stained with methyl green-pyronin Y (Taft 1951). The number of nuclei was counted using a hemocytometer under a microscope.…”

Section: Isolation Of Nucleimentioning

confidence: 99%

Chilling stress suppresses chloroplast development and nuclear gene expression in leaves of mung bean seedlings

Yang

Chen

Lin

et al. 2004

Planta

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Etiolated leaves of 28 degrees C-dark-grown mung bean (Vigna radiata L. cv. 2937) seedlings fail to turn green after being shifted to a light and cold environment. At the visible phenotypic level, incapability of leaf greening is the only failure event for the de-etiolation of mung bean seedlings at low temperature. Ultrastructural studies revealed that chloroplast development was completely suppressed by chilling treatment. A cDNA library originating from 28 degrees C-light-grown seedling leaves was constructed for screening cold-suppressed (cos) genes. Thirteen full-length cDNA clones were obtained, with 12 clones encoding chloroplast proteins, which, according to their known physiological functions, were important for chloroplast development and photosynthesis. Another cos cDNA encodes CYP90A2, which is a cytochrome P450 protein involved in the biosynthesis of brassinosteroid hormones. All cos genes are light-regulated at normal temperature. The influence of chilling stress on cos expression was examined in 10 degrees C-light- and 10 degrees C-dark-grown etiolated seedlings, and in 10 degrees C-light-grown green plants. The data show that cos expression in these three treatments is severely suppressed. This suppression is controlled at the transcriptional level, as demonstrated by nuclear runoff experiments, and is reversible because cos mRNAs accumulate again after the cold-treated plants have been transferred to 28 degrees C.

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“…The basic dye methyl green (septamethylpararosaniline), under certain conditions, can combine selectively with DNA (23,(34)(35)(36)(37). Experiments with various nucleic acid preparations in vitro led to the conclusion that methyl green combines specifically with polymerized DNA (34), and in definite stoichiometric proportion with the DNA orthophosphate groups, 1 mole of dye being bound per 10 atoms of P (35) presumably in the formation of a dye-nucleate salt.…”

Section: The A~nity Of Methyl Green For Dna--mentioning

confidence: 99%