The Species Pair Concept in the Lichen Dendrographa leucophaea (Arthoniales): Analyses Based on ITS Sequences

Lohtander, Katileena; Myllys, Leena; Sundin, Rikard; Källersjö, Mari; Tehler, Anders

doi:10.1639/0007-2745(1998)101[404:tspcit]2.0.co;2

Cited by 96 publications

(61 citation statements)

References 13 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Dilutions of total DNA were used for PCR amplification of the genes coding for the nuclear ITS rDNA and part of the protein-coding β-tubulin gene. Fungal nuITS rDNA was amplified using the primers ITS1F (Gardes and Bruns 1993), ITS4 (White et al 1990), ITS1-LM (Myllys et al 1999) and ITS2-KL (Lohtander et al 1998). The partial β-tubulin sequence was amplified using Bt3-LM and Bt10-LM primers (Myllys et al 2001).…”

Section: Dna Extraction and Pcr Amplificationmentioning

confidence: 99%

Another example of cryptic diversity in lichen-forming fungi: the new species Parmelia mayi (Ascomycota: Parmeliaceae)

et al. 2011

View full text Add to dashboard Cite

In the last decade, a number of cryptic species have been discovered in lichenized fungi, especially in species with a cosmopolitan or disjunctive distribution. Parmelia saxatilis is one of the most common and widely distributed species. Recent molecular studies have detected two species, P. ernstiae and P. serrana, within P. saxatilis s. lat., suggesting the existence of considerable genetic diversity that may not yet be expressed at the phenotypic level. Due to the complexity in the P. saxatilis s. lat. group, we used this as a model to study the species boundary and identify cryptic lineages. We used Phylogenetic (Bayes, ML and MP) and genetic distance approaches to analyze ITS and β-tubulin sequences. Our results confirm the existence of another cryptic lineage within P. saxatilis s. lat. This lineage is described herein as a new species, P. mayi. It forms an independent, strongly supported, monophyletic lineage, distantly related to the morphologically similar species P. ernstiae, P. saxatilis and P. serrana. Morphologically, it is indistinguishable from P. saxatilis but the new species is separated by molecular, bioclimatic, biogeographic and chemical characters. At present, P. mayi appears to have a restricted distribution in the northern Appalachian mountain territories of North America. It is found in climatic conditions ranging from hemiboreal and orotemperate to cryorotemperate ultrahyperhumid bioclimates.

show abstract

Section: Dna Extraction and Pcr Amplificationmentioning

confidence: 99%

Another example of cryptic diversity in lichen-forming fungi: the new species Parmelia mayi (Ascomycota: Parmeliaceae)

et al. 2011

View full text Add to dashboard Cite

show abstract

“…Dilutions of 1:10 of the total DNA were used for PCR amplifications of the nu ITS rDNA and mt SSU rDNA regions. Fungal nu ITS rDNA was amplified using the primers ITS1F (Gardes & Bruns 1993), ITS4 (White et al 1990) and, ITS1-LM (Myllys et al 1999) and ITS2-KL (Lohtander et al 1998) only in one sample (number 6, K301), while mt SSU rDNA was amplified using the primers mrSSU1 and mrSSU3R (Zoller et al 1999), MSU1 and MSU7 (Zhou & Stanosz 2001). Amplifications were performed in 50 l volumes containing a reaction mixture of 5 l 10 DNA polymerase buffer (Biotools) (containing 2 mM MgCl 2 , 10 mM Tris-HCl, pH 8·0, 50 mM KCl, 1 mM EDTA, 0·1% Triton X-100), 1 l of deoxynucleotide triphosphate (dNTPs), containing 10 mM of each base, 2·5 l of each primer (10 M), 1.25 l of DNA polymerase (1 U/ l) and 27·5 l dH 2 O.…”

Section: Dna Extraction and Pcr Amplificationmentioning

confidence: 99%

Molecular phylogenetic studies on the Parmotrema reticulatum (syn. Rimelia reticulata) complex, including the confirmation of P. pseudoreticulatum as a distinct species

et al. 2005

View full text Add to dashboard Cite

Molecular phylogenetic analyses based on nuclear ITS rDNA and mitochondrial SSU rDNA sequences from 32 specimens representing Parmotrema pseudoreticulatum, P. reticulatum and P. clavuliferum from Africa, Asia, Australia, and Europe are reported. Samples from western Europe and South Africa formed an independent monophyletic group, which belongs to P. pseudoreticulatum, a species not accepted by some recent authors, that has only been reported from Portugal, Spain and Morocco. In contrast, P. reticulatum and P. clavuliferum, both widely distributed taxa, formed a monophyletic clade, supporting the synonymy already proposed on the basis of morphological features.

show abstract

“…In both studies, monophyletic species consisted of multiple chemical races, a finding consistent with chemical polymorphism among interbreeding populations. Lohtander et al (80,82) and analyzed variable sequences from sexually and vegetatively reproducing forms of Dendrographa leucophaea and Roccellina capensis as well as species pairs of R. canariensis and R. tuberculata, Physcia distorta and Physcia detersa, and Physcia aipolia and Physcia caesia. They supported the repeated, even frequent, origin of the vegetatively reproducing forms with species pairs interpreted as members of a single phylogenetic species.…”

Section: Phylogenetic Analysis Of Chemospecies and Species Pairsmentioning

confidence: 99%

Early Molecular Investigations of Lichen-Forming Symbionts: 1986–2001

DePriest

2004

Annu. Rev. Microbiol.

View full text Add to dashboard Cite

From the mid-1980s the symbionts in lichen associations, heterotrophic fungi and photosynthetic algae or cyanobacteria, were the subject of increasing numbers of molecular investigations. Many of the studies examined the phylogenetic placement of the individual symbiotic partners with their free-living relatives, refining their nomenclature and classification. Resulting phylogenies permitted the mapping of transitions to and from lichenization and stimulated discussion of the relative ease of gaining and losing symbiotic lifestyles. Comparing symbiont phylogenies both rejected strict cospeciation and mirrored phylogenies and hinted at more complex forces of coevolution, including symbiont switching and selection. Studies at the species and population levels examined patterns of species delimitation and geographic dispersion and processes such as gene flow, self-fertilization, and founder effect. Significant genetic variation often was associated with mobile elements, group I and spliceosomal introns. This review examines the influence of molecular investigation on lichenology during this first 15 years.

show abstract

The Species Pair Concept in the Lichen Dendrographa leucophaea (Arthoniales): Analyses Based on ITS Sequences

Cited by 96 publications

References 13 publications

Another example of cryptic diversity in lichen-forming fungi: the new species Parmelia mayi (Ascomycota: Parmeliaceae)

Another example of cryptic diversity in lichen-forming fungi: the new species Parmelia mayi (Ascomycota: Parmeliaceae)

Molecular phylogenetic studies on the Parmotrema reticulatum (syn. Rimelia reticulata) complex, including the confirmation of P. pseudoreticulatum as a distinct species

Early Molecular Investigations of Lichen-Forming Symbionts: 1986–2001

Contact Info

Product

Resources

About