The spatiotemporal expression and mineralization regulation of p75 neurotrophin receptor in the early tooth development

Abstract

Objective: The aim of this study was to investigate the spatiotemporal expression and potential role of p75NTR in tooth morphogenesis and tissue mineralization. Materials and methods:The dynamic morphology of the four stages (from the beginning of E12.5 d, then E13.5 d and E15.5 d, to the end of E18.5 d) was observed, and the expressions of p75NTR and Runx2 were traced. The ectomesenchymal stem cells (EMSCs) were harvested in vitro, and the biological characteristics were observed.Moreover, the mineralization capability of EMSCs was evaluated. The relations between p75NTR and ALP, Col-1 and Runx2 were investigated. Results:The morphologic results showed that the dental lamina appeared at E12.5 d, the bud stage at E13.5 d, the cap stage at E15.5 d and the bell stage at E18.5 d.p75NTR and Runx2 showed the similar expression pattern. EMSCs from the four stages showed no significant difference in proliferation. But the positive rate of p75NTR in the E12.5 d cells was significantly lower than that in the other three stages (P < 0.05). Moreover, the higher positive rate of p75NTR the cells were, the stronger mineralization capability they showed. p75NTR was well positively correlated with the mineralization-related markers ALP, Col-1 and Runx2, which increased gradually with the mature of dental germs.Conclusion: p75NTR might play an important role in the regulation of tooth morphogenesis, especially dental hard tissue formation.This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

The role and potential mechanism of p75NTR in mineralization via in vivo p75NTR knockout mice and in vitro ectomesenchymal stem cells

“…12 Moreover, p75NTR has been reported to participate in tooth morphogenesis and dental hard tissue mineralization. [13][14][15][16][17] However, most molecular signatures on the effect of p75NTR in tooth morphogenesis remain to be uncovered, and the elusive mechanism underlying tooth development has severely restricted dental tissue engineering thus far. In this study, p75N-TR ExIII -knockout mice and EMSCs isolated from p75NTR ExIII−/− and p75NTR +/+ male mice were investigated to reveal the exact mechanism of p75NTR in mineralization regulation, contributing to dental tissue engineering.…”

“…Based on a previous report, 16 this study continued to investigate the potential mechanism of p75NTR in regulating mineralization.…”

p75NTR−/− mice exhibit an alveolar bone loss phenotype and inhibited PI3K/Akt/β‐catenin pathway

“…It has been reported that p75NTR might participate in the regulation of rat tooth morphogenesis, especially dental hard tissue formation 21. Xing et al22 found that p75NTR‐positive EMSCs showed greater odontogenic differentiation ability than p75NTR‐negative EMSCs both in vivo and in vitro.…”

P75 neurotrophin receptor positively regulates the odontogenic/osteogenic differentiation of ectomesenchymal stem cells via nuclear factor kappa-B signaling pathway

“…Importantly, studies showed that p75NTR to be a specific surface marker of neural crest-derived stem cells [ 26 ]. Thus, it can be used to purify neural crest-derived EMSCs and to regulate tooth morphogenesis, osteogenic differentiation and tissue mineralization [ 11 , 27 , 28 ]. In this study, p75NTR +/+ EMSCs show a higher proliferation and odonto/osteogenic differentiation than p75NTR −/− EMSCs.…”