The spatio-temporal pattern of Wallerian degeneration in mammalian peripheral nerves

Donat, Jane F.; ́niewski, Henry M. Wis

doi:10.1016/0006-8993(73)90765-8

Cited by 101 publications

(27 citation statements)

References 14 publications

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“…When a neuron is destroyed, the associated axon undergoes Wallerian degeneration. Although the ultrastructural changes observed in the present study were mild, they are qualitatively similar to those observed during early Wallerian degeneration (Vial 1958, Donat & Wisniewski 1973. These non-specific changes are also common during inflammation, when a modification of the permeability of the nerve membrane can result in edema, due to an augmented influx of ions.…”

Section: Resultssupporting

confidence: 76%

Pathology of intracardiac nerves in experimental Chagas disease

Ribeiro

Barbosa

Andrade

2002

Mem. Inst. Oswaldo Cruz

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Section: Resultssupporting

confidence: 76%

Pathology of intracardiac nerves in experimental Chagas disease

Ribeiro

Barbosa

Andrade

2002

Mem. Inst. Oswaldo Cruz

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“…39 This finding is consistent with reports indicating that small fibers are less affected by injury, 40,41 but is contrary to other reports suggesting the opposite, [42][43][44][45] or suggesting no size preference. 46 …”

Section: Neurite Outgrowth In Cultured Neuronscontrasting

confidence: 55%

Consequences of Neurite Transection In Vitro

Cengiz

Öztürk

Erdoğan

et al. 2012

Journal of Neurotrauma

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In order to quantify degenerative and regenerative changes and analyze the contribution of multiple factors to the outcome after neurite transection, we cultured adult mouse dorsal root ganglion neurons, and with a precise laser beam, we transected the nerve fibers they extended. Cell preparations were continuously visualized for 24 h with time-lapse microscopy. More distal cuts caused a more elongated field of degeneration, while thicker neurites degenerated faster than thinner ones. Transected neurites degenerated more if the uncut neurites of the same neuron simultaneously degenerated. If any of these uncut processes regenerated, the transected neurites underwent less degeneration. Regeneration of neurites was limited to distal cuts. Unipolar neurons had shorter regeneration than multipolar ones. Branching slowed the regenerative process, while simultaneous degeneration of uncut neurites increased it. Proximal lesions, small neuronal size, and extensive and rapid neurite degeneration were predictive of death of an injured neuron, which typically displayed necrotic rather than apoptotic form. In conclusion, this in vitro model proved useful in unmasking many new aspects and correlates of mechanically-induced neurite injury.

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“…Also, axotomized neurons must switch from a transmitting mode to a growth mode, expressing growth-associated proteins, such as GAP-43, as well as different neuropeptides and cytokines (26). Axonal sprouts must reach the distal nerve stump at a time when its growth support is optimal (27,28). If such coordination does not occur, nerve regeneration may be diminished and neuronal death may occur.…”

Section: Introductionmentioning

confidence: 99%

Non-neuronal cells are not the limiting factor for the low axonal regeneration in C57BL/6J mice

Oliveira

Langone

2000

Braz J Med Biol Res

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Peripheral axonal regeneration was investigated in adult male mice of the C57BL/6J (C), BALB/cJ (B) and A/J (A) strains and in their F1 descendants using a predegenerated nerve transplantation model. Four types of transplants were performed: 1) isotransplants between animals of the C, B and A strains; 2) donors of the C strain and recipients of the C x B and C x A breeding; 3) donors of the B strain and recipients of the C x B breeding, and 4) donors of the A strain and recipients of the C x A breeding. Donors had the left sciatic nerve transected and two weeks later a segment of the distal stump was transplanted into the recipient. Four weeks after transplantation the regenerated nerves were used to determine the total number of regenerated myelinated fibers (TMF), diameter of myelinated fibers (FD) and myelin thickness (MT). The highest TMF values were obtained in the groups where C57BL/6J mice were the donors (C to F1 (C x B) = 4658 ± 304; C to F1 (C x A) = 3899 ± 198). Also, A/J grafts led to a significantly higher TMF (A to F1 (C x A) = 3933 ± 565). Additionally, isotransplant experiments showed that when the nerve is previously degenerated, C57BL/6J mice display the largest number of myelinated fibers (C to C = 3136 ± 287; B to B = 2759 ± 170, and A to A = 2835 ± 239). We also observed that when C57BL/6J was the graft donor, FD was the highest and MT did not differ significantly when compared with the other groups. These morphometric results reinforce the idea that Schwann cells and the nerve environment of C57BL/6J provide enough support to the regenerative process. In this respect, the present results support the hypothesis that the non-neuronal cells, mainly Schwann cells, present in the sciatic nerve of C57BL/6J mice are not the main limiting factor responsible for low axonal regeneration.

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The spatio-temporal pattern of Wallerian degeneration in mammalian peripheral nerves

Cited by 101 publications

References 14 publications

Pathology of intracardiac nerves in experimental Chagas disease

Pathology of intracardiac nerves in experimental Chagas disease

Consequences of Neurite Transection In Vitro

Non-neuronal cells are not the limiting factor for the low axonal regeneration in C57BL/6J mice

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