The spatial distribution of phospholipids and glycolipids in the membrane of the bacterium <i>Micrococcus luteus</i> varies during the cell cycle

Welby, Michèle; Poquet, Yannick; Tocanne, Jean‐François

doi:10.1016/0014-5793(96)00278-5

Cited by 36 publications

(19 citation statements)

References 22 publications

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“…In Cam‐treated cells the proteo‐lipid domain dissipates, as was recently shown by us utilizing other methods (2,10). Taken together with previous studies using spin and fluorescent membrane probes, enzyme activities (8,9), cross‐linking studies (7), our most recent (2,10) and present work give credence to the role of membrane domains in the bacterial cell cycle (alluded to in Introduction) and may contribute to “… the search of a new biomembrane model” (39).…”

Section: Discussionsupporting

confidence: 81%

Coexistence of Domains with Distinct Order and Polarity in Fluid Bacterial Membranes¶

Vanounou¹,

Pines²,

Pines³

et al. 2007

Photochemistry and Photobiology

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In this study we sought the detection and characterization of bacterial membrane domains. Fluorescence generalized polarization (GP) spectra of laurdan‐labeled Escherichia coli and temperature dependencies of both laurdan's GP and fluorescence anisotropy of 1,3‐diphenyl‐1,3,5‐hexatriene (DPH) (rDPH) affirmed that at physiological temperatures, the E. coli membrane is in a liquid‐crystalline phase. However, the strong excitation wavelength dependence of rlaurdan at 37°C reflects membrane heterogeneity. Time‐resolved fluorescence emission spectra, which display distinct biphasic redshift kinetics, verified the coexistence of two subpopulations of laurdan. In the initial phase, <50 ps, the redshift in the spectral mass center is much faster for laurdan excited at the blue edge (350 nm), whereas at longer time intervals, similar kinetics is observed upon excitation at either blue or red edge (400 nm). Excitation in the blue region selects laurdan molecules presumably located in a lipid domain in which fast intramolecular relaxation and low anisotropy characterize laurdan's emission. In the proteo‐lipid domain, laurdan motion and conformation are restricted as exhibited by a slower relaxation rate, higher anisotropy and a lower GP value. Triple‐Gaussian decomposition of laurdan emission spectra showed a sharp phase transition in the temperature dependence of individual components when excited in the blue but not in the red region. At least two kinds of domains of distinct polarity and order are suggested to coexist in the liquid‐crystalline bacterial membrane: a lipid‐enriched and a proteo‐lipid domain. In bacteria with chloramphenicol (Cam)–inhibited protein synthesis, laurdan showed reduced polarity and restoration of an isoemissive point in the temperature‐dependent spectra. These results suggest a decrease in membrane heterogeneity caused by Cam‐induced domain dissipation.

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Section: Discussionsupporting

confidence: 81%

Coexistence of Domains with Distinct Order and Polarity in Fluid Bacterial Membranes¶

Vanounou¹,

Pines²,

Pines³

et al. 2007

Photochemistry and Photobiology

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“…This septal domain might then generate tubular or other structures that would recruit and activate division proteins. This hypothesis is supported by evidence for cell cycle variations in the lipid composition of E. coli (Mozharov et al, 1985) and in the spatial distribution of the lipids of Micrococcus luteus (Welby et al, 1996), for the CL-rich domains at the division sites of E. coli (Mileykovskaya and Dowhan, 2000; Koppelman et al, 2001) and B. subtilis (Kawai et al, 2004), and the septal location of the polyunsaturated fatty acid eicosapentaenoic acid of Shewanella livingstonensis Ac10 (Sato et al, 2012). Significantly, distinct domains appear around and between the segregating chromosomes at a very early stage of the cell cycle in E. coli and these domains disappear when translation – and hence transertion – is abolished (Fishov and Woldringh, 1999).…”

Section: Hyperstructuresmentioning

confidence: 86%

The membrane: transertion as an organizing principle in membrane heterogeneity

et al. 2015

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The bacterial membrane exhibits a significantly heterogeneous distribution of lipids and proteins. This heterogeneity results mainly from lipid–lipid, protein–protein, and lipid–protein associations which are orchestrated by the coupled transcription, translation and insertion of nascent proteins into and through membrane (transertion). Transertion is central not only to the individual assembly and disassembly of large physically linked groups of macromolecules (alias hyperstructures) but also to the interactions between these hyperstructures. We review here these interactions in the context of the processes in Bacillus subtilis and Escherichia coli of nutrient sensing, membrane synthesis, cytoskeletal dynamics, DNA replication, chromosome segregation, and cell division.

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“…However, variations in the M. luteus lipid distribution have suggested the existence of phosphatidyl glycerol-rich and dimannosyl diacylglycerolrich domains within the membrane. 17 Among the methods developed for microbial characterization, the Fourier transform IR (FTIR) technique has several attractive features, because it is a rapid method requiring no extensive preparation of samples. 18 Moreover, it is becoming a powerful tool for analyzing cell components, as well as complex biological materials.…”

Section: Introductionmentioning

confidence: 99%

Fourier transform IR spectroscopic appraisal of radiation damage in Micrococcus luteus

et al. 2003

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Fourier transform IR spectroscopy (FTIR) is used to analyze cells of Micrococcus luteus, the type species of the highly heterogeneous genus Micrococcus that belongs to the Micrococcaceae family. The cells of M. luteus, which is a Gram-positive and yellow-pigmented bacterium, are submitted to increasing doses of gamma radiation. Irradiation leads to the generation of reactive oxygen species that induce biochemical changes as shown in spectral profiles. Beyond a dose of 0.70 kGy, significant differences between samples are observed, particularly in the 1485-900 cm(-1) region, which contains information about membrane lipids, cell wall polysaccharides, and nucleic acids. After a dose of 16.50 kGy, M. luteus is reincubated for times ranging from 1 to 24 h. Postirradiation reincubated bacteria are found far from the control and irradiated cells (mainly in the 985-900 cm(-1) range), suggesting that a biomolecular rearrangement occurs as soon as reincubation begins in the growth medium. Thus, FTIR spectroscopy appears to be a very useful technique for the rapid visualization of the alterations induced by both the radiation and mutagenic response during reincubation. The use of mathematical methods gives good insight into the biomolecular compounds involved in these two mechanisms. In view of these preliminary results, we hypothesize that it can be successfully applied to any type of tissue and that it may be a future interesting tool for evaluating the effects of radiation in humans.

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The spatial distribution of phospholipids and glycolipids in the membrane of the bacterium Micrococcus luteus varies during the cell cycle

Cited by 36 publications

References 22 publications

Coexistence of Domains with Distinct Order and Polarity in Fluid Bacterial Membranes¶

Coexistence of Domains with Distinct Order and Polarity in Fluid Bacterial Membranes¶

The membrane: transertion as an organizing principle in membrane heterogeneity

Fourier transform IR spectroscopic appraisal of radiation damage in Micrococcus luteus

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