The soybean NRAMP homologue, GmDMT1, is a symbiotic divalent metal transporter capable of ferrous iron transport

Kaiser, Brent N.; Moreau, Sophie; Castelli, Joanne; Thomson, Rowena; Lambert, Annie; Bogliolo, Stéphanie; Puppo, Alain; Day, David A.

doi:10.1046/j.1365-313x.2003.01802.x

Cited by 156 publications

(130 citation statements)

References 48 publications

(60 reference statements)

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“…This is a process necessarily mediated by transporters. Kaiser et al (2003) proposed the involvement of Nramp transporter GmDMT1. 12b However, biochemical evidence on Nramp transport suggests that its direction of transport is the opposite, towards, instead of from, the cytosol.…”

Section: Resultsmentioning

confidence: 99%

Iron distribution through the developmental stages of Medicago truncatula nodules

et al. 2013

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Paramount to symbiotic nitrogen fixation (SNF) is the synthesis of a number of metalloenzymes that use iron as a critical component of their catalytical core. Since this process is carried out by endosymbiotic rhizobia living in legume root nodules, the mechanisms involved in iron delivery to the rhizobia-containing cells are critical for SNF. In order to gain insight into iron transport to the nodule, we have used synchrotron-based X-ray fluorescence to determine the spatio-temporal distribution of this metal in nodules of the legume Medicago truncatula with hitherto unattained sensitivity and resolution. The data support a model in which iron is released from the vasculature into the apoplast of the infection/differentiation zone of the nodule (zone II). The infected cell subsequently takes up this apoplastic iron and delivers it to the symbiosome and the secretory system to synthesize ferroproteins.Upon senescence, iron is relocated to the vasculature to be reused by the shoot. These observations highlight the important role of yet to be discovered metal transporters in iron compartmentalization in the nodule and in the recovery of an essential and scarce nutrient for flowering and seed production.

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Section: Resultsmentioning

confidence: 99%

Iron distribution through the developmental stages of Medicago truncatula nodules

et al. 2013

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“…Nramp4 isolated from Thlaspi japonicum, a Ni hyperaccumulator, encodes a protein showing transport activity for Ni but not for Zn, Cd, or Mn in yeast (Mizuno et al, 2005). A soybean Nramp, DMT1 (for Divalent metal transporter1), can transport Fe, Mn, and possibly Zn and Cu (Kaiser et al, 2003). Nramp1 and Nramp3 from tomato are able to complement a yeast mutant defective in Mn uptake, smf1 (Bereczky et al, 2003), suggesting their involvement in Mn transport.…”

Section: Introductionmentioning

confidence: 99%

Nramp5 Is a Major Transporter Responsible for Manganese and Cadmium Uptake in Rice

et al. 2012

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Paddy rice (Oryza sativa) is able to accumulate high concentrations of Mn without showing toxicity; however, the molecular mechanisms underlying Mn uptake are unknown. Here, we report that a member of the Nramp (for the Natural ResistanceAssociated Macrophage Protein) family, Nramp5, is involved in Mn uptake and subsequently the accumulation of high concentrations of Mn in rice. Nramp5 was constitutively expressed in the roots and encodes a plasma membrane-localized protein. Nramp5 was polarly localized at the distal side of both exodermis and endodermis cells. Knockout of Nramp5 resulted in a significant reduction in growth and grain yield, especially when grown at low Mn concentrations. This growth reduction could be partially rescued by supplying high concentrations of Mn but not by the addition of Fe. Mineral analysis showed that the concentration of Mn and Cd in both the roots and shoots was lower in the knockout line than in wild-type rice. A short-term uptake experiment revealed that the knockout line lost the ability to take up Mn and Cd. Taken together, Nramp5 is a major transporter of Mn and Cd and is responsible for the transport of Mn and Cd from the external solution to root cells.

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“…Several additional transport processes on the SM have been identified, including proteins for transport of iron, zinc, calcium, and sulfate (22)(23)(24)(25)(26)(27). In addition, the movement of hydrogen ions has been reported through the activity of an H ϩ -ATPase (28 -30).…”

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confidence: 99%

Proteomic Analysis of the Soybean Symbiosome Identifies New Symbiotic Proteins*

Clarke

Loughlin

Gavrin

et al. 2015

Molecular & Cellular Proteomics

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Legumes form a symbiosis with rhizobia in which the plant provides an energy source to the rhizobia bacteria that it uses to fix atmospheric nitrogen. This nitrogen is provided to the legume plant, allowing it to grow without the addition of nitrogen fertilizer. As part of the symbiosis, the bacteria in the infected cells of a new root organ, the nodule, are surrounded by a plant-derived membrane, the symbiosome membrane, which becomes the interface between the symbionts. Fractions containing the symbiosome membrane (SM) and material from the lumen of the symbiosome (peribacteroid space or PBS) were isolated from soybean root nodules and analyzed using nongel proteomic techniques. Bicarbonate stripping and chloroform-methanol extraction of isolated SM were used to reduce complexity of the samples and enrich for hydrophobic integral membrane proteins. One hundred and ninety-seven proteins were identified as components of the SM, with an additional fifteen proteins identified from peripheral membrane and PBS protein fractions. Proteins involved in a range of cellular processes such as metabolism, protein folding and degradation, membrane trafficking, and solute transport were identified. These included a number of proteins previously localized to the SM, such as aquaglyceroporin nodulin 26, sulfate transporters, remorin, and Rab7 homologs. Among the proteome were a number of putative transporters for compounds such as sulfate, calcium, hydrogen ions, peptide/ dicarboxylate, and nitrate, as well as transporters for which the substrate is not easy to predict. Analysis of the promoter activity for six genes encoding putative SM proteins showed nodule specific expression, with five showing expression only in infected cells. Localization of two proteins was confirmed using GFP-fusion experiments. The data have been deposited to the ProteomeXchange with identifier PXD001132. This proteome will provide a rich resource for the study of the legumerhizobium symbiosis. Molecular & Cellular Proteomics

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The soybean NRAMP homologue, GmDMT1, is a symbiotic divalent metal transporter capable of ferrous iron transport

Cited by 156 publications

References 48 publications

Iron distribution through the developmental stages of Medicago truncatula nodules

Iron distribution through the developmental stages of Medicago truncatula nodules

Nramp5 Is a Major Transporter Responsible for Manganese and Cadmium Uptake in Rice

Proteomic Analysis of the Soybean Symbiosome Identifies New Symbiotic Proteins*

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