The Soybean Aphid Suction Trap Network: Sampling the Aerobiological “Soup”

Lagos‐Kutz, Doris; Voegtlin, David J.; Onstad, David W.; Hogg, David B.; Ragsdale, David W.; Tilmon, Kelley J.; Hodgson, Erin W.; DiFonzo, Christina; Groves, Russell L.; Krupke, Christian H.; LaForest, Joseph; Seiter, Nicholas J.; Duerr, Emily J.; Bradford, Benjamin Z; Hartman, G. L.

doi:10.1093/ae/tmaa009

Cited by 18 publications

(21 citation statements)

References 27 publications

(28 reference statements)

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“…Soybean thrips were captured in 2018 by the Suction Trap Network [22], which is located in Illinois (Freeport, Monmouth, Morris and Urbana-Champaign), Iowa (Ames, Kanawha, Nashua and Sutherland), Kansas (Manhattan), Louisiana (Chase), Michigan (Kellogg and Monroe), Minnesota (Lamberton, Morris and Rosemount), Missouri (Columbia), and Wisconsin (Arlington, Eau Claire and Hancock). Thrips were manually selected from the suction traps, transferred to 95% ethanol, and stored at −20 • C. Soybean thrips were identified based on the morphological characteristics included in Hoddle et al [23].…”

Section: Collection and Processing Of Soybean Thrips Samplesmentioning

confidence: 99%

Soybean Thrips (Thysanoptera: Thripidae) Harbor Highly Diverse Populations of Arthropod, Fungal and Plant Viruses

Thekke-Veetil

Lagos‐Kutz

McCoppin

et al. 2020

Viruses

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Soybean thrips (Neohydatothrips variabilis) are one of the most efficient vectors of soybean vein necrosis virus, which can cause severe necrotic symptoms in sensitive soybean plants. To determine which other viruses are associated with soybean thrips, the metatranscriptome of soybean thrips, collected by the Midwest Suction Trap Network during 2018, was analyzed. Contigs assembled from the data revealed a remarkable diversity of virus-like sequences. Of the 181 virus-like sequences identified, 155 were novel and associated primarily with taxa of arthropod-infecting viruses, but sequences similar to plant and fungus-infecting viruses were also identified. The novel viruses were predicted to have positive-sense RNA, negative-stranded RNA, double-stranded RNA, and single-stranded DNA genomes. The assembled sequences included 100 contigs that represented at least 95% coverage of a virus genome or genome segment. Sequences represented 12 previously described arthropod viruses including eight viruses reported from Hubei Province in China, and 12 plant virus sequences of which six have been previously described. The presence of diverse populations of plant viruses within soybean thrips suggests they feed on and acquire viruses from multiple host plant species that could be transmitted to soybean. Assessment of the virome of soybean thrips provides, for the first time, information on the diversity of viruses present in thrips.

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Section: Collection and Processing Of Soybean Thrips Samplesmentioning

confidence: 99%

Soybean Thrips (Thysanoptera: Thripidae) Harbor Highly Diverse Populations of Arthropod, Fungal and Plant Viruses

Thekke-Veetil

Lagos‐Kutz

McCoppin

et al. 2020

Viruses

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“…(Russian wheat aphid) and Aphis glycines Matsumura (soybean aphid), each of which had initially large populations that declined apparently due to the effects of natural enemies and other factors (Davis et al, 2014;Lagos-Kutz et al, 2020). However, we emphasize TA B L E 1 Covariate effects on species-level aphid abundance trends based on model averaging of the AIC c -best generalized linear mixed-effect models.…”

Section: Discussionmentioning

confidence: 99%

“…Data from the central USA (hereafter referred to as “Central”), were obtained from 53 suction trap sites with 85 aphid species identified between 2005 and 2019 by D. Voegtlin and D. Lagos‐Kutz. Central suction traps stand 5.8 m above the ground and sample 60 m 3 of air per minute (Lagos‐Kutz et al, 2020). The Central dataset is currently supported by the University of Georgia Center for Invasive Species and Ecosystem Health (“Bugwood Center”) as part of the Southern IPM Center's IPM Information Supplement funded by the US Department of Agriculture National Institute of Food and Agriculture.…”

Section: Methodsmentioning

confidence: 99%

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Complex life histories predispose aphids to recent abundance declines

Crossley

Smith

Davis

et al. 2021

Global Change Biology

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Many animals change feeding habits as they progress through life stages, exploiting resources that vary in space and time. However, complex life histories may bring new risks if rapid environmental change disrupts the timing of these switches. Here, we use abundance times series for a diverse group of herbivorous insects, aphids, to search for trait and environmental characteristics associated with declines. Our meta dataset spanned three world regions and >300 aphid species, tracked at 75 individual sites for 10–50 years. Abundances were generally falling, with median changes of −8.3%, −5.6%, and −0.1% per year in the central USA, northwestern USA, and United Kingdom, respectively. Aphids that obligately alternated between host plants annually and those that were agricultural pests exhibited the steepest declines, relative to species able to persist on the same host plant year‐round or those in natural areas. This suggests that host alternation might expose aphids to climate‐induced phenology mismatches with one or more of their host plant species, with additional risks from exposure to insecticides and other management efforts. Warming temperatures through time were associated with milder aphid declines or even abundance increases, particularly at higher latitudes. Altogether, while a warming world appeared to benefit some aphid species in some places, most aphid species that had time‐sensitive movements among multiple host plants seemed to face greater risk of decline. More generally, this suggests that recent human‐induced rapid environmental change is rebalancing the risks and rewards associated with complex life histories.

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“…PGbased pitfall traps were also proposed as a standard and minimally disturbing method to investigate the subterranean fauna of the mesovoid shallow substratum (López and Oromí 2010; emptied every six months). Since 2001, PG fixation is applied by the Soybean Aphid Suction Trap Network (STN;Lagos-Kutz et al 2020) for monitoring the 'aerobiological soup'. In the context of environmental genomic studies, Matos-Maraví et al (2019) proposed PG as the fixative of choice for mass samples originating from pitfall traps and flight interceptions, but neither tests nor metadata had been published along with this statement.…”

Section: Introductionmentioning

confidence: 99%

Application of propylene glycol in DNA-based studies of invertebrates

Weigand¹,

Desquiotz²,

Weigand³

et al. 2021

MBMG

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High-throughput sequencing (HTS) studies on invertebrates commonly use ethanol as the main sample fixative (upon collection) and preservative (for storage and curation). However, alternative agents exists, which should not be automatically neglected when studies are newly designed. This review provides an overview of the application of propylene glycol (PG) in DNA-based studies of invertebrates, thus to stimulate an evidence-based discussion. The use of PG in DNA-based studies of invertebrates is still limited (n = 79), but a steady increase has been visible since 2011. Most studies used PG as a fixative for passive trapping (73%) and performed Sanger sequencing (66%; e.g. DNA barcoding). More recently, HTS setups joined the field (11%). Terrestrial Coleoptera (30%) and Diptera (20%) were the most studied groups. Very often, information on the grade of PG used (75%) or storage conditions (duration, temperature) were lacking. This rendered direct comparisons of study results difficult, and highlight the need for further systematic studies on these subjects. When compared to absolute ethanol, PG can be more widely and cheaply acquired (e.g. as an antifreeze, 13% of studies). It also enables longer trapping intervals, being especially relevant at remote or hard-to-reach places. Shipping of PG-conserved samples is regarded as risk-free and is authorised, pinpointing its potential for larger trapping programs or citizen science projects. Its property to retain flexibility of morphological characters as well as to lead to a reduced shrinkage effect was especially appraised by integrative study designs. Finally, the so far limited application of PG in the context of HTS showed promising results for short read amplicon sequencing and reduced representation methods. Knowledge of the influence of PG fixation and storage for long(er) read HTS setups is currently unavailable. Given our review results and taking difficulties of direct methodological comparisons into account, future DNA-based studies of invertebrates should on a case-by-case basis critically scrutinise if the application of PG in their anticipated study design can be of benefit.

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The Soybean Aphid Suction Trap Network: Sampling the Aerobiological “Soup”

Cited by 18 publications

References 27 publications

Soybean Thrips (Thysanoptera: Thripidae) Harbor Highly Diverse Populations of Arthropod, Fungal and Plant Viruses

Soybean Thrips (Thysanoptera: Thripidae) Harbor Highly Diverse Populations of Arthropod, Fungal and Plant Viruses

Complex life histories predispose aphids to recent abundance declines

Application of propylene glycol in DNA-based studies of invertebrates

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