The South African Clawed Toad Xenopus laevis

Protection of Xenopus laevis Embryos Against Alcohol-induced Delayed Gut Maturation and Growth Retardation by Peroxiredoxin 5 and Catalase

“…The following criteria were recorded: (1) the number of normal, deformed and dead embryos; (2) the length of the body measured between the tip of the jaw and the tip of the tail; and (3) the developmental stage of the gut based on the looping of the intestine as described. 34 Animal cap explant assay for the detection of molecular markers by reverse transcription-PCR Animal caps were dissected from embryos at stages 8.5-9 and cultured in 67% Leibovitz's L-15 medium at pH 7.5 in the presence or in the absence of alcohol until stage 22. Total RNA was extracted from animal caps or whole embryos using TRIZOL (Invitrogen), in accordance with the manufacturer's instructions.…”

Comparison of RNA localization during oogenesis within Acipenser ruthenus and Xenopus laevis

“…Xenopus has continued to be an attractive model because it is easy to obtain, maintain, and produces large oocytes that can be easily manipulated and orientated due to the presence of pigmented granules at the animal pole. Currently there are nearly a thousand published studies about RNA localization using X. laevis oocytes (reviewed by Dettlaff and Rudneva, 1991; reviewed by Rasar and Hammes, 2006;National Library of Medicine, 2002). Raikova (1973), Raikova (1974) pointed out that during oogenesis, the frog oocytes share strong cytological similarities with sturgeons including sterlets (Acipenser ruthenus), such as the nucleolar and other nuclear structures, cytoplasmic organelles, the same structure of yolk platelets, presence of cortical granules, absence of ribosomes in previtellogenesis, extrusions of nucleolar material into the cytoplasm, and the same dense material cementing the mitochondria.…”

“…Xenopus has continued to be an attractive model because it is easy to obtain, maintain, and produces large oocytes that can be easily manipulated and orientated due to the presence of pigmented granules at the animal pole. Currently there are nearly a thousand published studies about RNA localization using X. laevis oocytes (reviewed by Dettlaff and Rudneva, 1991 ; reviewed by Rasar and Hammes, 2006 ; National Library of Medicine, 2002 ).…”