Ultraviolet (UV) radiation is a major cause of skin photoaging through generating excessive oxidative stress and inflammation. One of the strategies is to use photo‐chemoprotectors such as natural products with antioxidant and anti‐inflammatory properties to protect the skin from photo damage. The present study investigated the photoprotective potentials of topical administration of unhydrolyzed collagen, Epigallocatechin gallate (EGCG) and their combination against ultraviolet B (UVB)‐induced photoaging in nude mice. It was found that both the solo and combined pretreatments could recover UVB‐induced depletion of antioxidative enzymes, including superoxide dismutase (SOD) and glutathione peroxidase (GSH‐Px), as well as increase of lipid peroxide malondialdehyde (MDA) and inflammatory tumor necrosis factor‐α (TNF‐α). Meanwhile, the UVB‐stimulated skin collagen degradation was attenuated significantly with drug treatments, which was evidenced by expression analysis of matrix metalloproteinase‐1 (MMP‐1) and hydroxyproline (HYP). Additionally, the mouse skin histology showed that the drug‐pretreated groups possessed decreased epidermis thickness and normal collagen fiber structure of the dermis layer. These results demonstrated that EGCG and collagen both can protect the skin against UVB‐induced skin photoaging. Synergistically, the combination of them showed the maximum prevention to skin damage, showing its potential in the application of anti‐photoaging formulation products.This article is protected by copyright. All rights reserved