The Somatic Cell Genetics of Human Interferon: Assignment of Human Interferon Loci to Chromosomes 2 and 5

Tan, Yaosheng; Creagan, R.P.; Ruddle, Frank H.

doi:10.1073/pnas.71.6.2251

Cited by 56 publications

(33 citation statements)

References 19 publications

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“…The involvement of chromosomes 2 and 5 in the production of human interferon has already been shown (15). Karyotypic analysis of the MG-63 clones may provide additional information as to the chromosomal control of interferon production.…”

Section: Discussionmentioning

confidence: 99%

Human Interferon: Mass Production in a Newly Established Cell Line, MG-63

Billiau

Edy

Heremans

et al. 1977

Antimicrob Agents Chemother

260

126

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MG-63 cells, a line derived from an osteosarcoma, produced high yields of interferon after superinduction with polyinosinic acid·polycytidylic acid, cycloheximide, and actinomycin D. Advantages of MG-63 cells over diploid fibroblasts as a substrate are: no requirement for aging between confluency and induction, no requirement for priming, and 3.7-fold higher yields per square centimeter of culture surface. Physicochemically and biologically, MG-63 cell interferon resembles fibroblast rather than leukocyte interferon.

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Section: Discussionmentioning

confidence: 99%

Human Interferon: Mass Production in a Newly Established Cell Line, MG-63

Billiau

Edy

Heremans

et al. 1977

Antimicrob Agents Chemother

260

126

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“…The results of somatic cell hybrid studies, which required the expression of human interferon genes in cells, produced apparently confficting gene assignments (7,8,15,19 (5). The chromosomal assignment and refined localization of a and interferon genes should facilitate the study of interferon gene polymorphism and permit the determination of whether disorders involving interferon genes may have serious consequences to the host.…”

Section: Resultsmentioning

confidence: 99%

“…Somatic cell genetic studies with human/rodent hybrid cells segregating human chromosomes have resulted in assignment of ( interferon loci to chromosomes 2, 5, and 9 (7,8,15,19). These studies, based on the antiviral assay of human inteferons produced in induced (with virus or double-stranded RNA) human/mouse hybrid cells, were able to localize , but not a interferon loci, since interferons synthesized in human/mouse hybrid cells were identified as 1 type (by a neutralization test with appropriate polyclonal antibodies) regardless of the type of human cell used for fusion (7,8,15,19).…”

mentioning

confidence: 99%

Human beta interferon gene localization and expression in somatic cell hybrids.

Pitha¹,

Slate²,

Raj³

et al. 1982

Mol. Cell. Biol.

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The human fibroblast interferon gene beta 1 was mapped to human chromosome 9. Sequence homology with a beta 1 cDNA clone was detected in both genomic DNA and induced mRNA of human/mouse or human/hamster somatic cell hybrids containing human chromosome 9, but not in lines lacking this chromosome or those retaining a complex translocation involving chromosomes 9 and 11. Interferon mRNA that did not share sequence homology with the beta 1 cDNA clone was detected in lines containing human chromosomes 2 and 5 but lacking chromosome 9, suggesting the presence of other unlinked interferon sequences in the human genome.

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“…CTP34, both species are also induced and superinduced. In contrast, Tan et al (1974a) reported that their human-mouse hybrids with human lymphocyte parents were inducible for mouse and human interferons with NDV, but produced only human interferon in response to poly(rl). poly(rC).…”

Section: Production Of Interferons In Cell Hybrids and Parental Cellsmentioning

confidence: 98%