The solubilisation pattern of lutein, zeaxanthin, canthaxanthin and β-carotene differ characteristically in liposomes, liver microsomes and retinal epithelial cells

Shafaa, Medhat W.; Diehl, H.; Socaciu, Carmen

doi:10.1016/j.bpc.2007.05.007

Cited by 26 publications

(14 citation statements)

References 23 publications

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“…Lipid bilayers have attracted much interest as idealized model systems for cellular membranes mainly because many of their physical properties are close to those of natural membranes [58]. Although it was shown that the incorporation efficiencies of lutein, zeaxanthin and canthaxanthin into different types of membranes are different and the conclusion drawn that neither liposomes nor microsomes may be considered model for studies of the incorporation of carotenoids into retinal pigment epithelial (RPE) cells [59], only model studies on defined membranes can give insight into the molecular interactions between these pigments and the membrane lipids.…”

Section: Mechanisms Of Canthaxanthin Delivery To the Macula Lutea Memmentioning

confidence: 99%

Interactions between canthaxanthin and lipid membranes — possible mechanisms of canthaxanthin toxicity

Sujak

2009

Cellular and Molecular Biology Letters

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Canthaxanthin (β, β-carotene 4, 4' dione) is used widely as a drug or as a food and cosmetic colorant, but it may have some undesirable effects on human health, mainly caused by the formation of crystals in the macula lutea membranes of the retina. This condition is called canthaxanthin retinopathy. It has been shown that this type of dysfunction of the eye is strongly connected with damage to the blood vessels around the place of crystal deposition. This paper is a review of the experimental data supporting the hypothesis that the interactions of canthaxanthin with the lipid membranes and the aggregation of this pigment may be the factors enhancing canthaxanthin toxicity towards the macula vascular system. All the results of the experiments that have been done on model systems such as monolayers of pure canthaxanthin and mixtures of canthaxanthin and lipids, oriented bilayers or liposomes indicate a very strong effect of canthaxanthin on the physical properties of lipid membranes, which may explain its toxic action, which leads to the further development of canthaxanthin retinopathy.

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Section: Mechanisms Of Canthaxanthin Delivery To the Macula Lutea Memmentioning

confidence: 99%

Interactions between canthaxanthin and lipid membranes — possible mechanisms of canthaxanthin toxicity

Sujak

2009

Cellular and Molecular Biology Letters

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“…galloxanthin [19]; 1-carotene [9]; zeaxanthin [20,21]; and astaxanthin [9,22]. We also calculated the absorptance, A(l), connected to various peak absorbance values, D max , for an object containing galloxanthin, zeaxanthin or astaxanthin.…”

Section: Materials and Methods (A) Optical Parameters Of The Oil Dropletsmentioning

confidence: 99%

Oil droplets of bird eyes: microlenses acting as spectral filters

Stavenga

Wilts

2014

Phil. Trans. R. Soc. B

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An important component of the cone photoreceptors of bird eyes is the oil droplets located in front of the visual-pigment-containing outer segments. The droplets vary in colour and are transparent, clear, pale or rather intensely yellow or red owing to various concentrations of carotenoid pigments. Quantitative modelling of the filter characteristics using known carotenoid pigment spectra indicates that the pigments' absorption spectra are modified by the high concentrations that are present in the yellow and red droplets. The high carotenoid concentrations not only cause strong spectral filtering but also a distinctly increased refractive index at longer wavelengths. The oil droplets therefore act as powerful spherical microlenses, effectively channelling the spectrally filtered light into the photoreceptor's outer segment, possibly thereby compensating for the light loss caused by the spectral filtering. The spectral filtering causes narrow-band photoreceptor spectral sensitivities, which are well suited for spectral discrimination, especially in birds that have feathers coloured by carotenoid pigments.

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“…The resulting carotenoid solution in chloroform (5 ml) was evaporated on a rotary evaporator to a volume of 1 ml. Liposomes with carotenoids were obtained by the method given in the article by Shafaa et al (43) with slight changes. 100 μl of chloroform solution (20 mg/ml) of egg phosphatidylcholine (Avanti) was added to carotenoid solution in chloroform (1 ml), stirred and evaporated chloroform on a rotary evaporator.…”

Section: Production Of Liposomes With Carotenoidsmentioning

confidence: 99%

Soluble cyanobacterial carotenoprotein as a robust antioxidant nanocarrier and delivery module

Maksimov

Zamaraev

Parshina

et al. 2019

Preprint

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To counteract oxidative stress, antioxidants including carotenoids are highly promising, yet their exploitation is drastically limited by the poor bioavailability and fast photodestruction, whereas current delivery systems are far from being efficient. Here we demonstrate that the recently discovered nanometer-sized water-soluble carotenoprotein from Anabaena (termed CTDH) transiently interacts with liposomes to efficiently extract carotenoids via carotenoidmediated homodimerization, yielding violet-purple protein samples amenable to lyophilization and long-term storage. We characterize spectroscopic properties of the pigment-protein complexes and thermodynamics of liposome-protein carotenoid transfer and demonstrate the highly efficient delivery of echinenone form CTDH into liposomes. Most importantly, we show carotenoid delivery to membranes of mammalian cells, which provides protection from reactive oxygen species. The described carotenoprotein may be considered as part of modular systems for the targeted antioxidant delivery.Significance statement: Carotenoids are excellent natural antioxidants but their delivery to vulnerable cells is challenging due to their hydrophobic nature and susceptibility to degradation. Thus, systems securing antioxidant stability and facilitating targeted delivery are of great interest

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The solubilisation pattern of lutein, zeaxanthin, canthaxanthin and β-carotene differ characteristically in liposomes, liver microsomes and retinal epithelial cells

Cited by 26 publications

References 23 publications

Interactions between canthaxanthin and lipid membranes — possible mechanisms of canthaxanthin toxicity

Interactions between canthaxanthin and lipid membranes — possible mechanisms of canthaxanthin toxicity

Oil droplets of bird eyes: microlenses acting as spectral filters

Soluble cyanobacterial carotenoprotein as a robust antioxidant nanocarrier and delivery module

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