The Solanum tuberosum KST1 partial promoter as a tool for guard cell expression in multiple plant species

Kelly, Gilor; Lugassi, Nitsan; Belausov, Eduard; Wolf, Dalia; Khamaisi, Belal; Brandsma, Danja; Kottapalli, Jayaram; Fidel, Lena; Ben-Zvi, Batsheva; Egbaria, Aiman; Acheampong, Atiako Kwame; Zheng, Chuanlin; Or, Etti; Distelfeld, Assaf; David‐Schwartz, Rakefet; Carmi, Nir; Granot, David

doi:10.1093/jxb/erx159

Cited by 24 publications

(21 citation statements)

References 47 publications

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“…Despite the important advances obtained in this field in the last decades, several outstanding questions regarding the source of sucrose for guard cells and the role of sucrose during stomatal opening and closure remain to be explored, deserving special attention in the near future. Reverse genetic approaches using guard cell specific promoters 72,73 combined with physiological and metabolic flux analysis have provided important information to the field and thus may be further extrapolated to the manipulation of other genes of guard metabolism. In parallel, the establishment of new mathematical models and the improvement of those currently in use will certainly help to unravel key mechanisms that regulate stomatal movement.…”

Section: Discussionmentioning

confidence: 99%

Toward multifaceted roles of sucrose in the regulation of stomatal movement

Lima

Medeiros

Anjos

et al. 2018

Plant Signaling & Behavior

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Plant atmospheric CO fixation depends on the aperture of stomatal pores at the leaf epidermis. Stomatal aperture or closure is regulated by changes in the metabolism of the two surrounding guard cells, which respond directly to environmental and internal cues such as mesophyll-derived metabolites. Sucrose has been shown to play a dual role during stomatal movements. The sucrose produced in the mesophyll cells can be transported to the vicinity of the guard cells via the transpiration stream, inducing closure in periods of high photosynthetic rate. By contrast, sucrose breakdown within guard cells sustains glycolysis and glutamine biosynthesis during light-induced stomatal opening. Here, we provide an update regarding the role of sucrose in the regulation of stomatal movement highlighting recent findings from metabolic and systems biology studies. We further explore how sucrose-mediated mechanisms of stomatal movement regulation could be useful to understand evolution of stomatal physiology among different plant groups.

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Section: Discussionmentioning

confidence: 99%

Toward multifaceted roles of sucrose in the regulation of stomatal movement

Lima

Medeiros

Anjos

et al. 2018

Plant Signaling & Behavior

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“…We next used reverse transcription-quantitative PCR (RT-qPCR) expression analysis of the ABA reporter genes NCED3 (Kelly et al, 2017), RAB18 (Lång and Palva, 1992), and ABI5 (Lopez-Molina et al, 2001) to evaluate the homogeneity of the selected lines. These genes were expressed similarly in the three fa lines.…”

Section: Fbpase::abi1-1 Transgenesmentioning

confidence: 99%

“…The specificity of the primers was determined by a melting curve analysis; a single, sharp peak in the melting curve indicates that a single, specific DNA sequence was amplified. Arabidopsis TUB2 (b-tubulin; Kelly et al, 2017) was used as a reference for the standardization of quantities of cDNA.…”

Section: Physiological and Morphological Characterization Of Transgenmentioning

confidence: 99%

Mesophyll Abscisic Acid Restrains Early Growth and Flowering But Does Not Directly Suppress Photosynthesis

et al. 2019

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Abscisic acid (ABA) levels increase significantly in plants under stress conditions, and ABA is thought to serve as a key stressresponse regulator. However, the direct effect of ABA on photosynthesis and the effect of mesophyll ABA on yield under both well-watered and drought conditions are still the subject of debate. Here, we examined this issue using transgenic Arabidopsis (Arabidopsis thaliana) plants carrying a dominant ABA-signaling inhibitor under the control of a mesophyll-specific promoter (FBPase::abi1-1, abbreviated to fa). Under normal conditions, fa plants displayed slightly higher stomatal conductance and carbon assimilation than wild-type plants; however, these parameters were comparable following ABA treatment. These observations suggest that ABA does not directly inhibit photosynthesis in the short term. The fa plants also exhibited a variety of altered phenotypes under optimal conditions, including more vigorous initial growth, earlier flowering, smaller flowers, and delayed chlorophyll degradation. Furthermore, under optimal conditions, fa plant seed production was less than a third of that observed for the wild type. However, under drought conditions, wild-type and fa seed yields were similar due to a significant reduction in wild-type seed and no reduction in fa seed. These findings suggest that endogenous basal ABA inhibits a stress-escape response under nonstressed conditions, allowing plants to accumulate biomass and maximize yield. The lack of a correlation between flowering time and plant biomass combined with delayed chlorophyll degradation suggests that this stress-escape behavior is regulated independently and upstream of other ABA-induced effects such as rapid growth and flowering.

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“…To monitor the appearance of stomata on citrus flowers and fruits, we used transgenic T. citrange (C. sinensis "Washington" sweet orange × P. trifoliata) containing the KSTpro:GFP that drives guard-cell expression of GFP (Lugassi et al, 2015;Kelly et al, 2017). The immediate and constitutive expression of KSTpro:GFP in newly formed guard cells (Kelly et al, 2017) allowed us to monitor the appearance of stomata easily and accurately. We started at early stages of flower development, when the flowers were still closed.…”

Section: Temporal Formation and Spatial Distribution Of Stomata On Vamentioning

confidence: 99%

“…During the process of gas exchange, the plant transpires water vapor into the surrounding atmosphere and CO 2 for photosynthesis enters the plant. The spatial and temporal distribution of stomata on leaves has been studied extensively over the past decades (Geisler and Sack, 2002;Nadeau and Sack, 2002;Kelly et al, 2017). In Arabidopsis, stomata appear on the cotyledons immediately after germination and, soon after, on the edges of true leaves, and the densities of the stomata on those plant parts increase in the following days (Kelly et al, 2017).…”

Section: Introductionmentioning

confidence: 99%

Expression of Hexokinase in Stomata of Citrus Fruit Reduces Fruit Transpiration and Affects Seed Development

et al. 2020

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The temporal formation and spatial distribution of stomata on the surface of citrus floral organs and, specifically, on the ovule from which the fruit develops, were analyzed using citrus plants that express green fluorescent protein (GFP) under the guard cell-specific KST1 promoter. Stomata are found on the style, sepal, and anther of the closed flower and on ovules from the stage of anthesis. It has previously been shown that hexokinase (HXK) mediates sugar-sensing in leaf guard cells and stimulates stomatal closure. The activity and response of citrus fruit stomata to sugar-sensing by HXK was examined using plants that express HXK under the KST1 promoter. Those plants are referred to as GCHXK plants. The transpiration of young green GCHXK citrus fruits was significantly reduced, indicating that their stomata respond to sugar similar to leaf stomata. Toward fruit maturation, fruit stomata are plugged and stop functioning, which explains why WT and GCHXK mature yellow fruits exhibited similar water loss. Seeds of the GCHXK plants were smaller and germinated more slowly than the WT seeds. We suggest that the stomata of young green citrus fruits, but not mature yellow fruits, respond to sugar levels via HXK and that fruit stomata are important for proper seed development.

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The Solanum tuberosum KST1 partial promoter as a tool for guard cell expression in multiple plant species

Abstract: The KST1 partial promoter is expressed in guard cells immediately after guard mother cell division and drives constitutive guard cell expression in dicots and monocots, and under drought and ABA treatment.

Cited by 24 publications

References 47 publications

Toward multifaceted roles of sucrose in the regulation of stomatal movement

Toward multifaceted roles of sucrose in the regulation of stomatal movement

Mesophyll Abscisic Acid Restrains Early Growth and Flowering But Does Not Directly Suppress Photosynthesis

Expression of Hexokinase in Stomata of Citrus Fruit Reduces Fruit Transpiration and Affects Seed Development

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