The SNPs in the human genetic blueprint era

Giampaoli, Saverio; Chillemi, Giovanni; Valeriani, Federica; Lazzaro, Domenico; Borro, Marina; Gentile, Giovanna; Simmaco, Maurizio; Zanni, Ginevra; Berti, Andrea; Spica, Vincenzo Romano

doi:10.1016/j.nbt.2012.11.015

Cited by 8 publications

(2 citation statements)

References 139 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Molecular biology have revolutionized all medical fields, including forensics . In particular, the recent NGS technology seems to offer new opportunities connected with a strong high‐throughput capacity, reduced costs, and the possibility of simultaneous analysis of several genetic aspects of biological materials .…”

Section: Discussionmentioning

confidence: 99%

“…The recent availability of next‐generation sequencing (NGS) protocols for forensic applications has raised the question whether a real‐time PCR approach is still a viable method for the identification of vaginal fluids . In fact the NGS approach, through metagenomics analysis, is able to move the microbiological characterization of a sample to a deeper level, with the identification of thousands of taxa in a few days.…”

mentioning

confidence: 99%

See 1 more Smart Citation

Informativeness of NGS Analysis for Vaginal Fluid Identification

Giampaoli

DeVittori

Valeriani

et al. 2016

Journal of Forensic Sciences

View full text Add to dashboard Cite

The identification of vaginal fluids in forensic examinations plays an important role in crime scene reconstruction. Molecular detection of vaginal bacterial communities can lead to the correct discrimination of body fluids. These kinds of studies can be performed through multiplex real-time PCR using primers for a specific selection of bacteria. The availability of next-generation sequencing (NGS) protocols provided for the extension of the analysis to evaluate the prokaryotes present in specimens. In this study, DNA was extracted from 18 samples (vaginal, oral, fecal, yoghurt) and analyzed by real-time PCR and NGS. The comparison between the two approaches has demonstrated that the information developed through NGS can augment the more conventional real-time PCR detection of a few key bacterial species to provide a more probative result and the correct identification of vaginal fluid from samples that are more forensically challenged.

show abstract

Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%