The SNAP-25 Protein Family

Kádková, Anna; Radecke, Julika; Sørensen, Jakob Balslev

doi:10.1016/j.neuroscience.2018.09.020

Cited by 68 publications

(57 citation statements)

References 265 publications

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“…After demonstrating that SCGN promotes the proper subcellular distribution of SNAP-25, we next studied how SNAP-25 regulates the functions of SCGN. SNAP-25 is alternatively spliced, creating two isoforms, SNAP-25a and SNAP-25b (15). The two isoforms share most exons except for exon 5, encoding two proteins of same length but differing in nine amino acids.…”

Section: The Scgn-snap-25 Interaction Promotes Hormone Secretion Frommentioning

confidence: 99%

“…S11 and S13). Since both SNAP-25 and SNAP-23 are implicated in brain development (15,18), and most SCGN-contacting residues in SNAP-25 are conserved in SNAP-23 (SI Appendix, Fig. S6C), SCGN functions likely by interacting with SNAP-25 and SNAP-23, and by modulating synapse activity.…”

Section: Scgn Controls Zebrafish Neuronal Development and Brain Growthmentioning

confidence: 99%

“…In vertebrates, the SNAP-25 family also includes SNAP-23, SNAP-29, and SNAP-47 (15). Whereas both SNAP-25 and SNAP-23 function in driving regulated exocytosis, SNAP-29 and SNAP-47 regulate other cellular events.…”

mentioning

confidence: 99%

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Structural and mechanistic insights into secretagogin-mediated exocytosis

Qin

Liu

et al. 2020

Proc. Natl. Acad. Sci. U.S.A.

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Secretagogin (SCGN) is a hexa–EF-hand protein that is highly expressed in the pancreas, brain, and gastrointestinal tract. SCGN is known to modulate regulated exocytosis in multiple cell lines and tissues; however, its exact functions and underlying mechanisms remain unclear. Here, we report that SCGN interacts with the plasma membrane SNARE SNAP-25, but not the assembled SNARE complex, in a Ca2+-dependent manner. The crystal structure of SCGN in complex with a SNAP-25 fragment reveals that SNAP-25 adopts a helical structure and binds to EF-hands 5 and 6 of SCGN. SCGN strongly inhibits SNARE-mediated vesicle fusion in vitro by binding to SNAP-25. SCGN promotes the plasma membrane localization of SNAP-25, but not Syntaxin-1a, in SCGN-expressing cells. Finally, SCGN controls neuronal growth and brain development in zebrafish, likely via interacting with SNAP-25 or its close homolog, SNAP-23. Our results thus provide insights into the regulation of SNAREs and suggest that aberrant synapse functions underlie multiple neurological disorders caused by SCGN deficiency.

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Section: The Scgn-snap-25 Interaction Promotes Hormone Secretion Frommentioning

confidence: 99%

Section: Scgn Controls Zebrafish Neuronal Development and Brain Growthmentioning

confidence: 99%

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Structural and mechanistic insights into secretagogin-mediated exocytosis

Qin

Liu

et al. 2020

Proc. Natl. Acad. Sci. U.S.A.

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“…Another role for ATP is in phosphorylation of protein substrates important for exocytosis. This topic is reviewed elsewhere in detail (Klenchin and Martin, 2000; Laidlaw et al, 2017; Kadkova et al, 2018).…”

Section: The Role Of Atp In Exocytosismentioning

confidence: 99%

Unraveling the mechanisms of calcium-dependent secretion

Anantharam

Kreutzberger

2019

Journal of General Physiology

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Ca2+-dependent secretion is a process by which important signaling molecules that are produced within a cell—including proteins and neurotransmitters—are expelled to the extracellular environment. The cellular mechanism that underlies secretion is referred to as exocytosis. Many years of work have revealed that exocytosis in neurons and neuroendocrine cells is tightly coupled to Ca2+ and orchestrated by a series of protein–protein/protein–lipid interactions. Here, we highlight landmark discoveries that have informed our current understanding of the process. We focus principally on reductionist studies performed using powerful model secretory systems and cell-free reconstitution assays. In recent years, molecular cloning and genetics have implicated the involvement of a sizeable number of proteins in exocytosis. We expect reductionist approaches will be central to attempts to resolve their roles. The Journal of General Physiology will continue to be an outlet for much of this work, befitting its tradition of publishing strongly mechanistic, basic research.

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“…GPR3 is 274 thought to be constitutively active and no ligand has yet been identified [51,52], but there is still a 275 possibility that the oocyte produces and secretes one. Because SNAP23 participates in constitutive and regulated exocytosis in other cell types [53,54], we 281 investigated if it is required for cortical granule exocytosis in mouse eggs using Trim-away. For these 282 experiments, we co-injected immature oocytes with RNA encoding TRIM21 and an antibody against 283 SNAP23 and matured the oocytes for ~18 hrs.…”

mentioning

confidence: 99%

SNAP23 is required for the maintenance of meiotic arrest and cortical granule exocytosis in mouse oocytes

Lm¹,

Tf²,

Km³

2019

Preprint

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Mammalian oocytes are stored in the ovary for prolonged periods, arrested in meiotic prophase. During this period, their plasma membranes are constantly being recycled by endocytosis and exocytosis.However, the function of this membrane turnover is unknown. Here, we investigated the requirement for exocytosis in the maintenance of meiotic arrest. Using Trim-away, a newly developed method for rapidly and specifically depleting proteins in oocytes, we have identified the SNARE protein, SNAP23, to be required for meiotic arrest. Degradation of SNAP23 causes premature meiotic resumption in follicle-enclosed oocytes. The reduction in SNAP23 is associated with loss of gap junction communication between the oocyte and surrounding follicle cells. Reduction of SNAP23 protein also inhibits regulated exocytosis in response to a Ca 2+ stimulus (cortical granule exocytosis), as measured by lectin staining and cleavage of ZP2. Our results show an essential role for SNAP23 in two key processes that occur in mouse oocytes and eggs.

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The SNAP-25 Protein Family

Cited by 68 publications

References 265 publications

Structural and mechanistic insights into secretagogin-mediated exocytosis

Structural and mechanistic insights into secretagogin-mediated exocytosis

Unraveling the mechanisms of calcium-dependent secretion

SNAP23 is required for the maintenance of meiotic arrest and cortical granule exocytosis in mouse oocytes

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