The smart and gentle microscope

Scherf, Nico; Huisken, Jan

doi:10.1038/nbt.3310

Cited by 93 publications

(76 citation statements)

References 26 publications

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“…Overall, the points mentioned above confer the LSFM technique with unprecedented capabilities of imaging in a quantitative way, and a wide range of biological processes in a huge variety of samples [1,104].…”

Section: 1g Lsfm Allows Multimodal Imaging Of Biological Samplesmentioning

confidence: 99%

Light-sheet microscopy: a tutorial

et al. 2018

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This paper is intended to give a comprehensive review of light-sheet (LS) microscopy from an optics perspective. As such, emphasis is placed on the advantages that LS microscope configurations present, given the degree of freedom gained by uncoupling the excitation and detection arms. The new imaging properties are first highlighted in terms of optical parameters and how these have enabled several biomedical applications. Then, the basics are presented for understanding how a LS microscope works. This is followed by a presentation of a tutorial for LS microscope designs, each working at different resolutions and for different applications. Then, based on a numerical Fourier analysis and given the multiple possibilities for generating the LS in the microscope (using Gaussian, Bessel, and Airy beams in the linear and nonlinear regimes), a systematic comparison of their optical performance is presented. Finally, based on advances in optics and photonics, the novel optical implementations possible in a LS microscope are highlighted.

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Section: 1g Lsfm Allows Multimodal Imaging Of Biological Samplesmentioning

confidence: 99%

Light-sheet microscopy: a tutorial

et al. 2018

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“…Typically, the microscope surveys the plate for an event of interest and then switches to an acquisition mode that is generally more data intensive, such as high-resolution 3D stacks, fast livecell imaging, super-resolution microscopy, total internal reflectance (TIRF), or fluorescence recovery after photobleaching (FRAP). 10,11 Currently, smart microscopy on commercial systems is restricted to low-resolution scans of a plate followed by high-resolution imaging of objects of interest (prescan/rescan), and the other modalities are currently not commercially available.…”

Section: Novel Screening Modalities and Readoutsmentioning

confidence: 99%

SLAS Europe High-Content Screening Conference in Dresden: A Glimpse of the Future?

Montoya

Bickle

2016

SLAS Discovery

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“…Despite the advent of optical imaging techniques, 3D and 4D (3D spatial + 1D time) methodologies for visualization and analysis of developmental cardiac mechanics and physiology lack user-directed platforms (1)(2)(3). While emerging efforts are being devoted to integrate advanced imaging techniques with interactive visualization methods (4-8), we further coupled interactive interrogation with quantification of microenvironments to uncover ultrastructure and function at high spatiotemporal resolution.…”

Section: Introductionmentioning

confidence: 99%