The small untranslated RNA SR1 from the <i>Bacillus subtilis</i> genome is involved in the regulation of arginine catabolism

Heidrich, Nadja; Chinali, Alberto; Gerth, Ulf; Brantl, Sabine

doi:10.1111/j.1365-2958.2006.05384.x

Cited by 110 publications

(172 citation statements)

References 57 publications

(110 reference statements)

Supporting

Mentioning

167

Contrasting

Order By: Relevance

“…SR1 inhibits the translation of ahrC, which encodes the transcriptional activator of the rocABC and rocDEF operons involved in arginine catabolism (104). The interaction between SR1 and ahrC is atypical in the sense that this sRNA does not bind to the 5= end of the cognate mRNA.…”

Section: Independently Expressed Small Rnas and Rna Antitoxinsmentioning

confidence: 99%

Regulatory RNAs in Bacillus subtilis: a Gram-Positive Perspective on Bacterial RNA-Mediated Regulation of Gene Expression

Mars

Nicolas

Denham

et al. 2016

Microbiol Mol Biol Rev

View full text Add to dashboard Cite

SUMMARY Bacteria can employ widely diverse RNA molecules to regulate their gene expression. Such molecules include trans -acting small regulatory RNAs, antisense RNAs, and a variety of transcriptional attenuation mechanisms in the 5′ untranslated region. Thus far, most regulatory RNA research has focused on Gram-negative bacteria, such as Escherichia coli and Salmonella . Hence, there is uncertainty about whether the resulting insights can be extrapolated directly to other bacteria, such as the Gram-positive soil bacterium Bacillus subtilis . A recent study identified 1,583 putative regulatory RNAs in B. subtilis , whose expression was assessed across 104 conditions. Here, we review the current understanding of RNA-based regulation in B. subtilis , and we categorize the newly identified putative regulatory RNAs on the basis of their conservation in other bacilli and the stability of their predicted secondary structures. Our present evaluation of the publicly available data indicates that RNA-mediated gene regulation in B. subtilis mostly involves elements at the 5′ ends of mRNA molecules. These can include 5′ secondary structure elements and metabolite-, tRNA-, or protein-binding sites. Importantly, sense-independent segments are identified as the most conserved and structured potential regulatory RNAs in B. subtilis . Altogether, the present survey provides many leads for the identification of new regulatory RNA functions in B. subtilis .

show abstract

Section: Independently Expressed Small Rnas and Rna Antitoxinsmentioning

confidence: 99%

Regulatory RNAs in Bacillus subtilis: a Gram-Positive Perspective on Bacterial RNA-Mediated Regulation of Gene Expression

Mars

Nicolas

Denham

et al. 2016

Microbiol Mol Biol Rev

View full text Add to dashboard Cite

show abstract

“…131) This protein also represses sr1, 132) which encodes a small non-coding regulatory RNA that inhibits the translation of ahrC encoding a transcriptional regulator that activates the rocABC and rocDEF operons for arginine catabolism and represses the gene cluster for arginine biosynthesis. 108,[133][134][135] CcpN is active when cells are growing on a glycolytic substrate, even if the medium also contains a gluconeogenic substrate. 127) CcpN is inhibited on interaction with YqfL, which is active during gluconeogenesis, and pckA and gapB as well as sr1 are derepressed.…”

Section: Catabolite Control Mediated By Ccpb Ccpc Ccpn and Cggrmentioning

confidence: 99%

Carbon Catabolite Control of the Metabolic Network inBacillus subtilis

Fujita

2009

Bioscience, Biotechnology, and Biochemistry

257

284

View full text Add to dashboard Cite

“…Although the B. subtilis ymaH gene encodes a protein homologous to Hfq, this protein is not required for FsrAmediated inhibition of Sdh expression because a fur ymaH double mutant does not regain the ability to grow on succinate. To date, the function of Hfq remains unclear in B. subtilis and it is apparently not needed for the function of other known sRNAs (37,38). Perhaps because of the extensive sequence complementarity between FsrA and the sdhC mRNA (Fig.…”

Section: Iron-sparing Response Reduces Cellular Iron Demand and Is Admentioning

confidence: 99%

“…Both target RNA (5Ј 166 sdhC mRNA) and fsrA were synthesized in vitro from PCR-generated template fragments, using a T7 RNA polymerase kit (Ambion) and purified from 6% denaturing polyacrylamide gels according to the manufacturer's instructions. RNA-RNA complex formation was measured according to (38), using 6% native polyacrylamide gels containing 1ϫ TBE. Dried gels were analyzed by PhosphoImaging.…”

Section: Quantification Of Target Genesmentioning

confidence: 99%

The Bacillus subtilis iron-sparing response is mediated by a Fur-regulated small RNA and three small, basic proteins

Gaballa

Antelmann

Aguilar

et al. 2008

Proc. Natl. Acad. Sci. U.S.A.

198

243

View full text Add to dashboard Cite

Regulation of bacterial iron homeostasis is often controlled by the iron-sensing ferric uptake repressor (Fur). The Bacillus subtilis Fur protein acts as an iron-dependent repressor for siderophore biosynthesis and iron transport proteins. Here, we demonstrate that Fur also coordinates an iron-sparing response that acts to repress the expression of iron-rich proteins when iron is limiting. When Fur is inactive, numerous iron-containing proteins are downregulated, including succinate dehydrogenase, aconitase, cytochromes, and biosynthetic enzymes for heme, cysteine, and branched chain amino acids. As a result, a fur mutant grows slowly in a variety of nutrient conditions. Depending on the growth medium, rapid growth can be restored by mutations in one or more of the molecular effectors of the iron-sparing response. These effectors include the products of three Fur-regulated operons that encode a small RNA (FsrA) and three small, basic proteins (FbpA, FbpB, and FbpC). Extensive complementarity between FsrA and the leader region of the succinate dehydrogenase operon is consistent with an RNA-mediated translational repression mechanism for this target. Thus, iron deprivation in B. subtilis activates pathways to remodel the proteome to preserve iron for the most critical cellular functions.

show abstract

The small untranslated RNA SR1 from the Bacillus subtilis genome is involved in the regulation of arginine catabolism

Cited by 110 publications

References 57 publications

Regulatory RNAs in Bacillus subtilis: a Gram-Positive Perspective on Bacterial RNA-Mediated Regulation of Gene Expression

Regulatory RNAs in Bacillus subtilis: a Gram-Positive Perspective on Bacterial RNA-Mediated Regulation of Gene Expression

Carbon Catabolite Control of the Metabolic Network inBacillus subtilis

The Bacillus subtilis iron-sparing response is mediated by a Fur-regulated small RNA and three small, basic proteins

Contact Info

Product

Resources

About