The small RNA-viruses of insects

Moore, Norman; Tinsley, T. W.

doi:10.1007/bf01315220

Cited by 43 publications

(22 citation statements)

References 92 publications

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“…Because only a single polyadenylated viral mRNA species could be detected in CrPV-infected cells (Fig. 1B) (39), the structural proteins are likely to be translated from the genomic RNA, excluding only the first possibility.…”

Section: Resultsmentioning

confidence: 99%

Naturally Occurring Dicistronic Cricket Paralysis Virus RNA Is Regulated by Two Internal Ribosome Entry Sites

Wilson

Powell

Hoover

et al. 2000

Molecular and Cellular Biology

292

335

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Cricket paralysis virus is a member of a group of insect picorna-like viruses. Cloning and sequencing of the single plus-strand RNA genome revealed the presence of two nonoverlapping open reading frames, ORF1 and ORF2, that encode the nonstructural and structural proteins, respectively. We show that each ORF is preceded by one internal ribosome entry site (IRES). The intergenic IRES is located 6,024 nucleotides from the 5 end of the viral RNA and is more active than the IRES located at the 5 end of the RNA, providing a mechanistic explanation for the increased abundance of structural proteins relative to nonstructural proteins in infected cells. Mutational analysis of this intergenic-region IRES revealed that ORF2 begins with a noncognate CCU triplet. Complementarity of this CCU triplet with sequences in the IRES is important for IRES function, pointing to an involvement of RNA-RNA interactions in translation initiation. Thus, the cricket paralysis virus genome is an example of a naturally occurring, functionally dicistronic eukaryotic mRNA whose translation is controlled by two IRES elements located at the 5 end and in the middle of the mRNA. This finding argues that eukaryotic mRNAs can express multiple proteins not only by polyprotein processing, reinitiation and frameshifting but also by using multiple IRES elements.

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Section: Resultsmentioning

confidence: 99%

Naturally Occurring Dicistronic Cricket Paralysis Virus RNA Is Regulated by Two Internal Ribosome Entry Sites

Wilson

Powell

Hoover

et al. 2000

Molecular and Cellular Biology

292

335

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show abstract

“…wt. of about 65000 (Matthews, 1982;Moore & Tinsley, 1982). To date, seven members, and potentially a further nine (Hendry et al, 1984), have been assigned to this group, on the basis of their serological relatedness to NflV and the size of their capsid proteins (Reinganum et al, 1978;Morris et al, 1979;Moore et al, 1981 ;.…”

mentioning

confidence: 99%

Small RNA Viruses Co-infecting the Pine Emperor Moth (Nudaurelia cytherea capensis)

Hendry

Hodgson

Clark

et al. 1985

Journal of General Virology

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“…This short review is restricted to these three families. Other small RNA viruses from insects have been described by Longworth (1978), Harrap & Payne (1979) and Moore & Tinsley (1982).…”

Section: Introductionmentioning

confidence: 99%

“…While small RNA viruses of insects appear to have a wide diversity of morphology and genome size (for reviews, see Longworth, 1978;Moore & Tinsley, 1982; only three groups have been studied in detail. These are the insect picornaviruses, and members of the Nodaviridae and the Nudaurelia [3 virus family.…”

Section: Introductionmentioning

confidence: 99%

General Characteristics, Gene Organization and Expression of Small RNA Viruses of Insects

Moore

Reavy

King

1985

Journal of General Virology

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The small RNA-viruses of insects

Cited by 43 publications

References 92 publications

Naturally Occurring Dicistronic Cricket Paralysis Virus RNA Is Regulated by Two Internal Ribosome Entry Sites

Naturally Occurring Dicistronic Cricket Paralysis Virus RNA Is Regulated by Two Internal Ribosome Entry Sites

Small RNA Viruses Co-infecting the Pine Emperor Moth (Nudaurelia cytherea capensis)

General Characteristics, Gene Organization and Expression of Small RNA Viruses of Insects

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