The small RNA RybA regulates key-genes in the biosynthesis of aromatic amino acids under peroxide stress in<i>E. coli</i>

Gerstle, Kirstin; Klätschke, Kristin; Hahn, Ulrich; Piganeau, Nicolas

doi:10.4161/rna.19065

Cited by 23 publications

(19 citation statements)

References 46 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…For example, Mn 2+ uptake and temporal TCA shutdown, driven by Fur under iron starvation condition, consequently led to an increase in the intracellular Fe 2+ pool 36 , and Fur-mediated repression of biofilm formation contributes to protecting cells from acidic environments caused by temporal TCA shutdown under this condition. Thus, disrupting the Fur regulatory network in either iron-replete or iron starvation conditions can lead to dramatic changes in transcript levels of genes.…”

Section: Discussionmentioning

confidence: 99%

Deciphering Fur transcriptional regulatory network highlights its complex role beyond iron metabolism in Escherichia coli

et al. 2014

View full text Add to dashboard Cite

The ferric uptake regulator (Fur) plays a critical role in the transcriptional regulation of iron metabolism. However, the full regulatory potential of Fur remains undefined. Here we comprehensively reconstruct the Fur transcriptional regulatory network in Escherichia coli K-12 MG1655 in response to iron availability using genome-wide measurements (ChIP-exo and RNA-seq). Integrative data analysis reveals that a total of 81 genes in 42 transcription units are directly regulated by three different modes of Fur regulation, including apo- and holo-Fur activation and holo-Fur repression. We show that Fur connects iron transport and utilization enzymes with negative-feedback loop pairs for iron homeostasis. In addition, direct involvement of Fur in the regulation of DNA synthesis, energy metabolism, and biofilm development is found. These results show how Fur exhibits a comprehensive regulatory role affecting many fundamental cellular processes linked to iron metabolism in order to coordinate the overall response of E. coli to iron availability.

show abstract

Section: Discussionmentioning

confidence: 99%

Deciphering Fur transcriptional regulatory network highlights its complex role beyond iron metabolism in Escherichia coli

et al. 2014

View full text Add to dashboard Cite

show abstract

“…RutR was demonstrated to be involved in glutamate-dependent acid resistance of E. coli for survival under acidic conditions34. In addition, many regulatory networks of the pathogen contain ncRNAs, which could also regulate the expression of key protein-coding genes during the adaptive response to environmental stimuli3637. We identified a non-synonymous mutation in Kpn2146_2018 (P184S), which belongs to the transcriptional regulator LysR family.…”

Section: Discussionmentioning

confidence: 99%

Genomic and transcriptomic analysis of NDM-1 Klebsiella pneumoniae in spaceflight reveal mechanisms underlying environmental adaptability

Liu

Wang

et al. 2014

Sci Rep

View full text Add to dashboard Cite

The emergence and rapid spread of New Delhi Metallo-beta-lactamase-1 (NDM-1)-producing Klebsiella pneumoniae strains has caused a great concern worldwide. To better understand the mechanisms underlying environmental adaptation of those highly drug-resistant K. pneumoniae strains, we took advantage of the China's Shenzhou 10 spacecraft mission to conduct comparative genomic and transcriptomic analysis of a NDM-1 K. pneumoniae strain (ATCC BAA-2146) being cultivated under different conditions. The samples were recovered from semisolid medium placed on the ground (D strain), in simulated space condition (M strain), or in Shenzhou 10 spacecraft (T strain) for analysis. Our data revealed multiple variations underlying pathogen adaptation into different environments in terms of changes in morphology, H2O2 tolerance and biofilm formation ability, genomic stability and regulation of metabolic pathways. Additionally, we found a few non-coding RNAs to be differentially regulated. The results are helpful for better understanding the adaptive mechanisms of drug-resistant bacterial pathogens.

show abstract

“…Waters et al [32] identified a small protein, MntS, that may act as a manganese chaperone, and they showed that overproduction of MntS resulted in an increase of entC expression when E. coli was grown with a high manganese concentration. Gerstle et al [33] showed that the RNA product of this gene, which they named RybA, can function as a small regulatory RNA. RybA, down-regulates aromatic amino acid biosynthesis under peroxide stress, which could increase the availability of chorismate to other biosynthetic pathways, including enterobactin production.…”

Section: Ferric Iron Transportmentioning

confidence: 99%

Regulation of iron transport systems in Enterobacteriaceae in response to oxygen and iron availability

Carpenter

Payne

2014

Journal of Inorganic Biochemistry

View full text Add to dashboard Cite

Iron is an essential nutrient for most bacteria. Depending on the oxygen available in the surrounding environment, iron is found in two distinct forms: ferrous (FeII) or ferric (FeIII). Bacteria utilize different transport systems for the uptake of the two different forms of iron. In oxic growth conditions, iron is found in its insoluble, ferric form, and in anoxic growth conditions iron is found in its soluble, ferrous form. Enterobacteriacea, have adapted to transporting the two forms of iron by utilizing the global, oxygen-sensing regulators, ArcA and Fnr to regulate iron transport genes in response to oxygen.

show abstract

The small RNA RybA regulates key-genes in the biosynthesis of aromatic amino acids under peroxide stress inE. coli

Cited by 23 publications

References 46 publications

Deciphering Fur transcriptional regulatory network highlights its complex role beyond iron metabolism in Escherichia coli

Deciphering Fur transcriptional regulatory network highlights its complex role beyond iron metabolism in Escherichia coli

Genomic and transcriptomic analysis of NDM-1 Klebsiella pneumoniae in spaceflight reveal mechanisms underlying environmental adaptability

Regulation of iron transport systems in Enterobacteriaceae in response to oxygen and iron availability

Contact Info

Product

Resources

About