The small RNA PrrH of Pseudomonas aeruginosa regulates hemolysis and oxidative resistance in bloodstream infection

Zeng, Shenghe; Shi, Qixuan; Liu, YinZhen; Li, Mo; Lin, Dongling; Zhang, Shebin; Li, Qiwei; Pu, Jieying; Cong, Shan; Huang, Bin; Cha, Chen; Zeng, Jianming

doi:10.1016/j.micpath.2023.106124

Cited by 3 publications

(3 citation statements)

References 62 publications

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“…coli O157 : H7 [ 52 ]. Study have found that sRNA PrrH negatively regulates katA to reduce the oxidative tolerance of Pseudomonas aeruginosa [ 53 ]. To further understand the role of sRNA51 in KPC-2-producing CRKP, we used IntaRNA 2.0.3 to predict its target genes, including yaiY , gabT , msrA , acrA , insA1 , and holE .…”

Section: Discussionmentioning

confidence: 99%

“…Jia et al found a novel sRNA EsrF that directly binds to the flagellar biosynthetic gene flhB to regulate its expression, thereby altering the motility and virulence of enterohemorrhagic E. coli O157: H7 [52]. Study have found that sRNA PrrH negatively regulates katA to reduce the oxidative tolerance of Pseudomonas aeruginosa [53]. To further understand the role of sRNA51 in KPC-2-producing CRKP, we used IntaRNA 2.0.3 to predict its target genes, including yaiY, gabT, msrA, acrA, insA1, and holE.…”

Section: Plos Pathogensmentioning

confidence: 99%

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sRNA expression profile of KPC-2-producing carbapenem-resistant Klebsiella pneumoniae: Functional role of sRNA51

Bai,

Xie,

Zhang

et al. 2024

PLoS Pathog

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The emergence of carbapenem-resistant Klebsiella pneumoniae (CRKP) has significant challenges to human health and clinical treatment, with KPC-2-producing CRKP being the predominant epidemic strain. Therefore, there is an urgent need to identify new therapeutic targets and strategies. Non-coding small RNA (sRNA) is a post-transcriptional regulator of genes involved in important biological processes in bacteria and represents an emerging therapeutic strategy for antibiotic-resistant bacteria. In this study, we analyzed the transcription profile of KPC-2-producing CRKP using RNA-seq. Of the 4693 known genes detected, the expression of 307 genes was significantly different from that of carbapenem-sensitive Klebsiella pneumoniae (CSKP), including 133 up-regulated and 174 down-regulated genes. Both the Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment and Gene Ontology (GO) analysis showed that these differentially expressed genes (DEGs) were mainly related to metabolism. In addition, we identified the sRNA expression profile of KPC-2-producing CRKP for the first time and detected 115 sRNAs, including 112 newly discovered sRNAs. Compared to CSKP, 43 sRNAs were differentially expressed in KPC-2-producing CRKP, including 39 up-regulated and 4 down-regulated sRNAs. We chose sRNA51, the most significantly differentially expressed sRNA in KPC-2-producing CRKP, as our research subject. By constructing sRNA51-overexpressing KPC-2-producing CRKP strains, we found that sRNA51 overexpression down-regulated the expression of acrA and alleviated resistance to meropenem and ertapenem in KPC-2-producing CRKP, while overexpression of acrA in sRNA51-overexpressing strains restored the reduction of resistance. Therefore, we speculated that sRNA51 could affect the resistance of KPC-2-producing CRKP by inhibiting acrA expression and affecting the formation of efflux pumps. This provides a new approach for developing antibiotic adjuvants to restore the sensitivity of CRKP.

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Section: Discussionmentioning

confidence: 99%

Section: Plos Pathogensmentioning

confidence: 99%

sRNA expression profile of KPC-2-producing carbapenem-resistant Klebsiella pneumoniae: Functional role of sRNA51

Bai,

Xie,

Zhang

et al. 2024

PLoS Pathog

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“…Bile salts are known to be substrates of RND efflux pumps, and the growth impairment could be due to the lack of efflux of these detergents and their effect on the integrity of membranes [20,21]. Although 2,2 ′ -dipyridyl is broadly used to study the effect of iron limitation on bacterial physiology [22][23][24], it could be a substrate of RND and other efflux pumps, and the inhibition of the growth of the deletion strains could be due to the intracellular accumulation of this compound and not due to iron deficiency. Therefore, we also tested whether ∆3 and other deletion mutants could grow in the M9 citrate minimal medium with and without a ferric iron supplement (Figure S7).…”

Section: Rnd Pumps Dominate the Growth Phenotypes Of A Baumannii Unde...mentioning

confidence: 99%

Acinetobacter baumannii Survival under Infection-Associated Stresses Depends on the Expression of Resistance–Nodulation–Division and Major Facilitator Superfamily Efflux Pumps

Leus,

Olvera,

Adamiak

et al. 2023

Antibiotics

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Multidrug efflux transporters are major contributors to the antibiotic resistance of Acinetobacter baumannii in clinical settings. Previous studies showed that these transporters are tightly integrated into the physiology of A. baumannii and have diverse functions. However, for many of the efflux pumps, such functions remain poorly defined. In this study, we characterized two putative drug efflux pumps, AmfAB and AmfCD (Acinetobacter Major Facilitator), that are homologous to EmrAB-like transporters from Escherichia coli and other Gram-negative bacteria. These pumps comprise the Major Facilitator Superfamily (MFS) transporters AmfB and AmfD and the periplasmic membrane fusion proteins AmfA and AmfC, respectively. We inactivated and overproduced these pumps in the wild-type ATCC 17978 strain and its derivative strains lacking the major efflux pumps from the Resistance–Nodulation–Division (RND) superfamily and characterized antibiotic susceptibilities and growth of the strains under stresses typical during human infections. We found that neither AmfAB nor AmfCD contribute to the antibiotic non-susceptibility phenotypes of A. baumannii. The two pumps, however, are critical for the adaptation and growth of the bacterium under acidic stress, whereas AmfCD also contributes to growth under conditions of low iron, high temperature, and in the presence of bile salts. These functions are dependent on the presence of the RND pumps, the inactivation of which further diminishes A. baumannii survival and growth. Our results suggest that MFS transporters contribute to stress survival by affecting the permeability properties of the A. baumannii cell envelope.

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Relationship between Pyochelin and Pseudomonas Quinolone Signal in Pseudomonas aeruginosa: A Direction for Future Research

Ma,

Zeng,

Xiao

et al. 2024

IJMS

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Pseudomonas aeruginosa is an opportunistic pathogen that requires iron to survive in the host; however, the host immune system limits the availability of iron. Pyochelin (PCH) is a major siderophore produced by P. aeruginosa during infection, which can help P. aeruginosa survive in an iron-restricted environment and cause infection. The infection activity of P. aeruginosa is regulated by the Pseudomonas quinolone signal (PQS) quorum-sensing system. The system uses 2-heptyl-3-hydroxy-4-quinolone (PQS) or its precursor, 2-heptyl-4-quinolone (HHQ), as the signal molecule. PQS can control specific life processes such as mediating quorum sensing, cytotoxicity, and iron acquisition. This review summarizes the biosynthesis of PCH and PQS, the shared transport system of PCH and PQS, and the regulatory relationship between PCH and PQS. The correlation between the PQS and PCH is emphasized to provide a new direction for future research.

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The small RNA PrrH of Pseudomonas aeruginosa regulates hemolysis and oxidative resistance in bloodstream infection

Cited by 3 publications

References 62 publications

sRNA expression profile of KPC-2-producing carbapenem-resistant Klebsiella pneumoniae: Functional role of sRNA51

sRNA expression profile of KPC-2-producing carbapenem-resistant Klebsiella pneumoniae: Functional role of sRNA51

Acinetobacter baumannii Survival under Infection-Associated Stresses Depends on the Expression of Resistance–Nodulation–Division and Major Facilitator Superfamily Efflux Pumps

Relationship between Pyochelin and Pseudomonas Quinolone Signal in Pseudomonas aeruginosa: A Direction for Future Research

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