The small RNA‐mediated gene silencing machinery is required in Arabidopsis for stimulation of growth, systemic disease resistance, and suppression of the nitrile‐specifier gene <i>NSP4</i> by <i>Trichoderma atroviride</i>

Rebolledo-Prudencio, Oscar Guillermo; Rivera, Magnolia Estrada; Castro, Mitzuko Dautt; Arteaga-Vázquez, Mario; Arenas-Huertero, Catalina; Rosendo-Vargas, Maria Montserrat; Jin, Hailing; Casas-Flores, Sergio

doi:10.1111/tpj.15599

Cited by 15 publications

(8 citation statements)

References 120 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Establishment of mutualistic associations between fungi and their host requires genetic and epigenetic reprogramming as well as metabolome modulation of both by the exchange of effector molecules [ 66 ]. Indeed, RdDM is essential in Arabidopsis to establish a beneficial relationship with the root-colonizing Trichoderma atroviride while DNA methylation and histone modifications are required for plant priming by the beneficial fungus against B. cinerea [ 67 ]. Importantly, it was just recently shown that during the mutualistic interaction of the ectomycorrhizal fungus Pisolithus microcarpus with Eucalyptus grandis , a fungal miRNA, Pmic_miR-8, targets the host NB-ARC domain containing transcripts in a cross-kingdom RNAi manner [ 68 ].…”

Section: Resultsmentioning

confidence: 99%

A beneficial fungal root endophyte triggers systemic RNA silencing and DNA methylation of a host reporter gene

et al. 2022

View full text Add to dashboard Cite

A growing body of evidence suggests that RNA interference (RNAi) plays a pivotal role in the communication between plants and pathogenic fungi, where a bi-directional trans-kingdom RNAi is established to the advantage of either the host or the pathogen. Similar mechanisms acting during plant association with non-pathogenic symbiotic microorganisms have been elusive to this date. To determine whether root endophytes can induce systemic RNAi responses to their host plants, we designed an experimental reporter-based system consisting of the root-restricted, beneficial fungal endophyte, Fusarium solani strain K (FsK) and its host Nicotiana benthamiana . Since not all fungi encode the RNAi machinery, we first needed to validate that FsK does so, by identifying its core RNAi enzymes (2 Dicer-like genes, 2 Argonautes and 4 RNA-dependent RNA polymerases) and by showing its susceptibility to in vitro RNAi upon exogenous application of double stranded RNAs (dsRNAs). Upon establishing this, we transformed FsK with a hairpin RNA (hpRNA) construct designed to target a reporter gene in its host N. benthamiana . The hpRNA was processed by FsK RNAi machinery predominantly into 21–24-nt small RNAs that triggered RNA silencing but not DNA methylation in the fungal hyphae. Importantly, when the hpRNA-expressing FsK was used to inoculate N. benthamiana , systemic RNA silencing and DNA methylation of the host reporter gene was recorded. Our data suggest that RNAi signals can be translocated by root endophytes to their hosts and can modulate gene expression during mutualism, which may be translated to beneficial phenotypes.

show abstract

Section: Resultsmentioning

confidence: 99%

A beneficial fungal root endophyte triggers systemic RNA silencing and DNA methylation of a host reporter gene

et al. 2022

View full text Add to dashboard Cite

show abstract

“…Establishment of mutualistic associations between fungi and their host requires genetic and epigenetic reprogramming as well as metabolome modulation of both by the exchange of effector molecules (Kloppholz et al ., 2011). Indeed, RdDM is essential in Arabidopsis to establish a beneficial relationship with the root-colonizing Trichoderma atroviride while DNA methylation and histone modifications are required for plant priming by the beneficial fungus against B. cinerea (Rebolledo-Prudencio et al ., 2021). Importantly, it was just recently shown that during the mutualistic interaction of the ectomycorrhizal fungus Pisolithus microcarpus with Eucalyptus grandis , a fungal miRNA, Pmic_miR-8, targets the host NB-ARC domain containing transcripts in a cross-kingdom RNAi manner (Wong-Bajracharya et al ., 2022).…”

Section: Resultsmentioning

confidence: 99%

Systemic silencing and DNA methylation of a host reporter gene induced by a beneficial fungal root endophyte

Dalakouras

Katsaouni

Avramidou

et al. 2022

Preprint

View full text Add to dashboard Cite

A growing body of evidence suggests that RNA interference (RNAi) plays a pivotal role in the communication between plants and pathogenic fungi, where a bi-directional cross-kingdom RNAi is established to the advantage of either the host or the pathogen. Similar mechanisms acting during plant association with non-pathogenic symbiotic microorganisms have been elusive to this date. Here, we report on an RNAi-based mechanism of communication between a beneficial fungal endophyte, Fusarium solani strain K (FsK) and its host plants. This soil-borne endophyte that confers resistance and/or tolerance to biotic and abiotic stress in tomato and, as shown in this study, promotes plant growth in Nicotiana benthamiana, is restricted to the root system in both host plants. We first showed that the fungus has a functional core RNAi machinery; double stranded RNAs (dsRNAs) are processed into short interfering RNAs (siRNAs) of predominantly 21-nt in size, which lead to the degradation of homologous mRNAs. Importantly, by using an RNAi sensor system, we demonstrated that root colonization of N. benthamiana by FsK led to the induction of systemic silencing and DNA methylation of a host reporter gene.. These data reflect a more general but so far unrecognized mechanism wherein root endophytes systemically translocate RNAi signals to the aboveground tissues of their hosts to modulate gene expression during symbiosis, which may be translated to the beneficial phenotypes.

show abstract

“…They provide a better defense mechanism against the aphid Macrosiphum euphorbiae and the moth Spodoptera littotalis in tomato plants [ 102 ], proving that T. atroviride may be able to control pathogens indirectly, modulating plant physiology. Besides modification of the transcription of genes involved in plant defense responses, colonization by T. atroviride also induces Arabidopsis ’s sRNA-mediated gene silencing, leading to an increase in gene expression of JA and SA-mediated pathways, which in turn induces priming and increases resistance in the plant against B. cinerea [ 103 ].…”

Section: Biocontrol Potential Of Registered Trichoderma ...mentioning

confidence: 99%

Trichoderma Species: Our Best Fungal Allies in the Biocontrol of Plant Diseases—A Review

Guzmán-Guzmán

Kumar

Santos-Villalobos

et al. 2023

Plants

View full text Add to dashboard Cite

Biocontrol agents (BCA) have been an important tool in agriculture to prevent crop losses due to plant pathogens infections and to increase plant food production globally, diminishing the necessity for chemical pesticides and fertilizers and offering a more sustainable and environmentally friendly option. Fungi from the genus Trichoderma are among the most used and studied microorganisms as BCA due to the variety of biocontrol traits, such as parasitism, antibiosis, secondary metabolites (SM) production, and plant defense system induction. Several Trichoderma species are well-known mycoparasites. However, some of those species can antagonize other organisms such as nematodes and plant pests, making this fungus a very versatile BCA. Trichoderma has been used in agriculture as part of innovative bioformulations, either just Trichoderma species or in combination with other plant-beneficial microbes, such as plant growth-promoting bacteria (PGPB). Here, we review the most recent literature regarding the biocontrol studies about six of the most used Trichoderma species, T. atroviride, T. harzianum, T. asperellum, T. virens, T. longibrachiatum, and T. viride, highlighting their biocontrol traits and the use of these fungal genera in Trichoderma-based formulations to control or prevent plant diseases, and their importance as a substitute for chemical pesticides and fertilizers.

show abstract

The small RNA‐mediated gene silencing machinery is required in Arabidopsis for stimulation of growth, systemic disease resistance, and suppression of the nitrile‐specifier gene NSP4 by Trichoderma atroviride

Cited by 15 publications

References 120 publications

A beneficial fungal root endophyte triggers systemic RNA silencing and DNA methylation of a host reporter gene

A beneficial fungal root endophyte triggers systemic RNA silencing and DNA methylation of a host reporter gene

Systemic silencing and DNA methylation of a host reporter gene induced by a beneficial fungal root endophyte

Trichoderma Species: Our Best Fungal Allies in the Biocontrol of Plant Diseases—A Review

Contact Info

Product

Resources

About