The Small-Angle Neutron Scattering Data Analysis of the Phospholipid Transport Nanosystem Structure

Abstract

Abstract. The small-angle neutron scattering technique (SANS) is employed for investigation of structure of the phospholipid transport nanosystem (PTNS) elaborated in the V.N.Orekhovich Institute of Biomedical Chemistry (Moscow, Russia). The SANS spectra have been measured at the YuMO small-angle spectrometer of IBR-2 reactor (Joint Institute of Nuclear Research, Dubna, Russia). Basic characteristics of polydispersed population of PTNS unilamellar vesicles (average radius of vesicles, polydispersity, thickness of membrane, etc.) have been determined in three cases of the PTNS concentrations in D2O: 5%, 10%, and 25%. Numerical analysis is based on the separated form factors method (SFF). The results are discussed in comparison with the results of analysis of the small-angle X-ray scattering spectra collected at the Kurchatov Synchrotron Radiation

Restoring structural parameters of lipid mixtures from small-angle X-ray scattering data

“…Various approaches exist to tackle the problem of modelling and fitting small-angle scattering data from model lipid membranes (Pabst et al, 2000;Pencer & Hallett, 2000;Pé rez & Koutsioubas, 2015;Skar-Gislinge et al, 2015;Heftberger et al, 2014;Zemlyanaya et al, 2016Zemlyanaya et al, , 2018Konarev et al, 2020). In most cases, scattering length density (SLD) profiles of symmetric lipid bilayers are represented in the form of step functions (Pencer & Hallett, 2000) or Gaussians (Pabst et al, 2000).…”

Drug Transport System Based on Phospholipid Nanoparticles: Production Technology and Characteristics

“…In the photo, one can see discrete particles that are predominantly spherical and/or rod-shaped. Previously, a combination of two methods-small-angle neutron scattering in the range of PhTNS concentration in heavy water of 5-25% by weight and small-angle scattering of X-ray synchrotron radiation in several concentrations in water-was used to determine the single-layer vesicular structure of phospholipid transport nanosystem (PhTNS) [23,24]. It was found that the size of vesicles decreased with an increase in the maltose concentration and was about 27.2 + 0.2 nm at a concentration of 20%.…”